Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 6 Issue 7

Utility of MALDI TOF AUTOF MS 1000 System for Identification and Authentication of Bacterial Strains using Standard ATCC Cultures

Kushal Gohil1 and John Kaundinya2*

1GeNei Laboratories, Pvt. Ltd., SRS College Road, Peenya Bangalore, India

2Rivaara, Inc., Carlsbad, CA, USA

*Corresponding Author: John Kaundinya, Rivaara, Inc., Carlsbad, CA, USA.

Received: May 15, 2023; Published: June 07, 2023


Precise bacterial identification depends on the reliability and efficiency of the technique being utilized. Scientists have incessantly adapted every advancement for better, quicker, and meticulous bacterial identification. MALDI-TOF MS is a microbial identification technique based on the soft ionization principle coupled with mass spectrometer. It became popular in this decade for its speed, uniformity, cost-effectiveness, ease of sample preparation, and accuracy. MALDI-TOF MS involves using a matrix to mix and bind the sample, which absorbs laser energy, causing fast heating, vaporization, and the ionization of the analytes; then the ions are distinguished based on the time they take to get to the detector, based on the principle that all ions with the same charge are given equal kinetic energy. Due to many diverse and extensive features of this exclusive detection method, this study principally aims to authenticate 38 bacterial strains using MALDI-TOF Autof MS1000 system. These strains included gram positive bacteria namely Enterococcus faecalis, Staphylococcus aureus, Listeria monocytogenes, Clostridioides difficile and gram-negative bacteria namely, Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Pseudomonas aeruginosa, Acinetobacter baumannii, and Hemophilus influenzae. The bacterial strains were accurately identified with a score value of more than 9.2 for all the strains, indicating high confidence in identification up to species level.

 Keywords: Matrix Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry (MS); Autobio MALDI TOF MS 1000; Rapid Bacterial Identification; Accurate Bacterial Detection; Peptide Mass Fingerprint (PMF)


  1. Doan NTL., et al. "Validation of MALDI‐TOF MS for rapid classification and identification of lactic acid bacteria, with a focus on isolates from traditional fermented foods in Northern Vietnam”. Letters in Applied Microbiology4 (2012): 265-273.
  2. Youse AE. "Detection of bacterial pathogens in different matrices: Current practices and challenges”. Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems(2008): 31-48.
  3. Bonnet M., et al. “Bacterial Culture through Selective and Non-Selective Conditions: The Evolution of Culture Media in Clinical Microbiology”. New Microbes and New Infections100622(2020): 100622.
  4. Franco-Duarte R., et al. "Advances in Chemical and Biological Methods to Identify Microorganisms—from Past to Present”. Microorganisms5 (2019): 130.
  5. Li D., et al. "Recent advances on aptamer-based biosensors for detection of pathogenic bacteria”. World Journal of Microbiology and Biotechnology37 (2021): 1-20.
  6. Li D., et al. "MALDI-TOF mass spectrometry in clinical analysis and research”. ACS Measurement Science Au5 (2022): 385-404.
  7. Han S-S., et al. "Current scenario and challenges in the direct identification of microorganisms using MALDI TOF MS”. Microorganisms9 (2021): 1917.
  8. Torres-Sangiao E., et al. "Application and perspectives of MALDI–TOF mass spectrometry in clinical microbiology laboratories”. Microorganisms7 (2021): 1539.
  9. Griffiths J. "A brief history of mass spectrometry”. Annals of Chemistry15 (2008): 5678-5683.
  10. Gandhi K., et al. ‘MALDITOF MS: Applications in Dairy and Related Sectors’. Res Rev2 (2018): 19-27.
  11. Singhal N., et al. "MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis”. Frontiers in Microbiology6 (2015): 791.
  12. Wang Y., et al. "Evaluation of VITEK MS, Clin-ToF-II MS, Autof MS 1000 and VITEK 2 ANC card for identification of Bacteroides fragilis group isolates and antimicrobial susceptibilities of these isolates in a Chinese university hospital”. Journal of Microbiology, Immunology and Infection3 (2019): 456-464.
  13. Ma Q., et al. "Evaluation of the Autof MS1000 mass spectrometer in the identification of clinical isolates”. BMC Microbiology20 (2020): 1-8.
  14. Wu H Y., et al. "Legionella qingyii nov., isolated from water samples in China”. International Journal of Systematic and Evolutionary Microbiology69.7 (2019): 2017-2022.
  15. Váradi L., et al. "Methods for the detection and identification of pathogenic bacteria: past, present, and future”. Chemical Society Reviews16 (2017): 4818-4832.
  16. Halebian S., et al. "Rapid method that aids in distinguishing Gram-positive from Gram-negative anaerobic bacteria”. Journal of Clinical Microbiology3 (1981): 444-448.


Citation: Kushal Gohil and John Kaundinya. “Utility of MALDI TOF AUTOF MS 1000 System for Identification and Authentication of Bacterial Strains using Standard ATCC Cultures". Acta Scientific Microbiology 6.7 (2023): 08-14.


Copyright: © 2023 Kushal Gohil and John Kaundinya. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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