Divya Sharma¹, Manpreet Kaur¹, Sunita Borkar² and Reena Gupta¹*

¹Department of Biotechnology, Himachal Pradesh University, Summerhill, Shimla, India
²Department of Microbiology, PES College of Arts and Science, Farmagudi, Ponda, Goa, India

*Corresponding Author: Reena Gupta, Professor, Department of Biotechnology, Himachal Pradesh University, Summerhill, Shimla, India. E-mail: reenagupta_2001@yahoo.com.

Received: May 08, 2023; Published: May 19, 2023

Abstract

In the present study, twenty-nine positive fungal isolates for production of laccase were obtained from decaying wood and soil samples from Shimla district of Himachal Pradesh, India. The positive isolates were confirmed by brown halo formation owing to oxidation of guaiacol by laccase enzyme. Out of the 29 isolates, the isolate PHF4, which produced the maximum laccase activity, was recognized as Beauveria pseudobassiana. Laccase displayed good activity in production medium comprising peptone (1.0%, w/v), malt extract (0.5%, w/v), glucose (2.0%, w/v) and CuSO₄ (0.005%, w/v). Optimization of inoculum size, incubation time, carbon source, carbon source concentration, nitrogen source, nitrogen source concentration, copper sulphate concentration, temperature and pH for laccase production was performed. The optimal inoculum size and incubation time for the laccase production were found to be 6 discs (1 cm in diameter) and 8 days respectively. Glucose (2.0%, w/v) and yeast extract (2.0%, w/v) were the most appropriate carbon and nitrogen source respectively for the production of enzyme. The presence of 0.003% (w/v) copper sulphate enhanced the laccase production. A temperature of 25°C and pH 6.0 were found to be optimum for laccase production. Under the optimum conditions, the maximum activity of enzyme was 21.47 U/ml which was 2.9 times higher than the unoptimized conditions. This is the first record of Beauveria pseudobassiana being isolated and optimized for the production of laccase.

 Keywords: Laccase; Optimization; Beauveria pseudobassiana; Guaiacol; Yeast Extract

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Citation

Citation: Divya Sharma., et al. “Screening and Identification of Novel Laccase Producing Entomopathogenic Fungus Beauveria pseudobassiana PHF4 and Optimization of Production Conditions of Enzyme". Acta Scientific Microbiology 6.6 (2023): 51-60.

Copyright

Copyright: © 2023 Reena Gupta., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.