Manore SG¹, Bagwan RA², Khan JV², Shaikh FY² and Salve AN³*

¹Department of Botany, Modern college of Arts, Science and Commerce, Shivajinagar Pune, India

*Corresponding Author: Salve AN, Department of Botany Government Institute of Science, Aurangabad, (M.S.) India.

Received: March 02, 2023; Published: May 19, 2023

Abstract

The experiment was performed to evaluate culture and growth performance of genus Dicrateria inornataParke. M. Three different growth media i.e., Natural Sea water, f/2 and Walne’s were selected for the comparative analysis. The culture flask containing 250ml media were inoculated in 50ml of axenic culture of D. inornataParke. M. The culture flask were incubated at 24 ± 2°C for 12 hours light and12 hours in dark photoperiod. The media at 5 different pH ranging from 5 to 9 were observed. The growth rate of D. inornataParke. M was analyzed by cell count method with the help of haemocytometer at an interval of 48 Hours. The maximum growth was observed at the end of 15^th day in Walne’s media (23.2 cells/100μl), in F/2 media (18.1 cells/100μl) and in sea water media (12.4cells/100μl) on 11^th day at pH 8. The Effect of different photoperiod on the growth of D. inornata Parke. M.in natural sea waterat 16hrs light and 8 hrs dark (11.8cells/100μl) maximum growth achieved on 11^th day. The maximum growth achieved on 15^th dayin F/2 media (18.1cell/100μl) and Walne’s media (24.9cells/100μl) at 16hrs light and 8hrs dark condition. Thus it can be concluded that at different pH the influence of chemical compounds on the growth of D. inornata Parke. M. varies.

 Keywords: D. inornata Parke. M., f/2 Media; Walne’s; Sea Water Media; Microalgae

References

1. Nzayisenga JC., et al. “Effects of light intensity on growth and lipid production in microalgae grown in wastewater”. Biotechnology for Biofuels 13:4 (2020): 2-8.
2. Bagwan RA., et al. “Growth studied of Synechocystis salina under different physical and chemical parameters”. Think India Journal 31 (2019): 723-728.
3. Singh SP and Priyanka Singh. “Effect of temperature and light on the growth of algae species: A review”. Renewable and Sustainable Energy (2015): 431-444.
4. Qiu R., et al. “Effects of pH on cell growth, lipid production and CO₂ addition of microalgae Chlorella sorokiniana”. Algal Research 28 (2017) 192-199.
5. Andersen RA. “Algal Culturing Techniques”. Phycological Society of America (2005).
6. Biswajit Rath and Indira Priyadarshani. “Commercial and industrial applications of microalgae- A review”. Journal of Algal Biomass Utilization 4 (2012): 89-100.
7. Hongju QI., et al. “A Comparative Study of the Sensitivity of Fv/Fm to Phosphorus Limitation on Four Marine Algae”. Oceanic and Coastal Sea Research 12 .1 (2013): 77-84.
8. Qiang Hu., et al. “Microalgal triacylglycerols as feed stocks for biofuel production: perspectives and advances”. The Plant Journal 54 (2008) 621-639.
9. Sharma R., et al. “Effects of Culture Conditions on Growth and Biochemical Profile of Chlorella Vulgaris”. Journal of Plant Pathology and Microbiology 131 (2012): 1-6.

Citation

Citation: Salve AN., et al. “Growth Studies of Dicrateria inornata Parke. M. under Different Physical and Chemical Parametersticulata Raw Material and its Extract: A Simple Method to Reduce the Microbial Load in Herbal Products". Acta Scientific Microbiology 6.6 (2023): 46-50.

Copyright

Copyright: © 2023 Salve AN., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.