Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 6 Issue 6

Microbiological Pattern of Surgical Site Infection Following Surgery at A Tertiary Level Hospital

Syeda Tamanna Tanjil1*, Mohammad Jayedul Islam2, Sharmin Akter Suma3, KM Shaiful Islam4 and Md Rassel5

1Consultant Clinical Pathologist, Department of Laboratory Medicine, SIBL Foundation Hospital, Dhaka, Bangladesh

2Junior Consultant, Department of Surgery, Shaheed Suhrawardy Medical College Hospital, Dhaka, Bangladesh

3Lecturer, Department of Anatomy, Shaheed Suhrawardy Medical College, Dhaka, Bangladesh

4Assistant Professor, Department of Pediatric Surgery, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh

5Associate Professor, Department of Surgical Oncology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh

*Corresponding Author: Syeda Tamanna Tanjil, Consultant Clinical Pathologist, Department of Laboratory Medicine, SIBL Foundation Hospital, Dhaka, Bangladesh Email:

Received: April 19, 2023; Published: May 03, 2023


Introduction: Surgical site infections (SSI) constitute a major public health problem worldwide and are the second most frequently reported nosocomial infections. They are responsible for increasing the treatment cost, length of hospital stays and significant morbidity and mortality.

Aim of the Study: The aim of the study was to determine the incidence of SSIs and the prevalence of aerobic bacterial pathogens involved with their antibiogram.

Materials and Methods: Samples were collected using sterile cotton swabs from 137 patients clinically diagnosed of having SSIs and were processed as per standard microbiological techniques. Antimicrobial susceptibility testing was done using modified Kirby-Bauer disc diffusion method. This cross sectional study was conducted for a period of six months (January 2020 to June 2020) in the Department of Microbiology at Shaheed Suhrawardy Medical College, Sher-E-Bangla Nagar, Dhaka.

Results: Out of 768 patients, 137 (17.8%) were found to have SSIs and samples were collected from them. Out of total 137 samples, 132 (96.4%) yielded bacterial growth and 139 bacterial isolates were obtained. Staphylococcus aureus (50.4%) was the commonest organism followed by Escherichia coli (23.02%), Pseudomonas aeruginosa (7.9%) and Citrobacter species (7.9%). Antimicrobial profile of gram positive isolates revealed maximum sensitivity to vancomycin, teicoplanin and linezolid, whereas among gram negative isolates meropenem, piperacillin-tazobactam, and amikacin were found to be most sensitive.

Conclusion: The rate of SSI observed in this study was comparable to other similar studies, however we observed a higher degree of antimicrobial resistance. Adherence to strict infection control measures, maintenance of proper hand hygiene and optimal preoperative, intraoperative and postoperative patient care will surely reduce the incidence of SSIs.

 Keywords: Surgical Site Infection; Wound Infections; Antibiotic Sensitivity; Meropenem; Staphylococcus


  1. Hohmann C., et al. “Adherence to guidelines for antibiotic prophylaxis in surgery patients in German hospitals: a multicentre evaluation involving pharmacy interns”. Infection2 (2012): 131-137.
  2. Owens CD and Stoessel K. “Surgical site infections: epidemiology, microbiology and prevention”. Journal of Hospital Infection 70 (2008): 3-10.
  3. Pradhan GB and Agrawal J. “Comparative study of post-operative wound infection following emergency lower segment caesarean section with and without the topical use offusidic acid”. Nepal Medical College Journal 3 (2009): 189-191.
  4. Ahmed MI. “Prevalence of nosocomial wound infection among postoperative patients and antibiotics patterns at teaching hospital in Sudan”. North American Journal of Medicine and Science 1 (2012): 29-34.
  5. Mulu W., et al. “Postoperative nosocomial infections and antimicrobial resistance patterns of bacterial isolates among patients admitted at FelegeHiwot Referral Hospital, Bahirdar, Ethiopia”. Ethiopian Journal of Health Science 1 (2012): 7-18.
  6. Masaadeh HA and Jaran AS. “Incident of Pseudomonas aeruginosa in post-operative wound infection”. American Journal of Infectious Diseases 5 (2009): 1-6.
  7. MacFaddin J. “Biochemical Tests for Identification of Medical Bacteria”. 3rd Philadelphia: Lippincott Williams and Wilkins; (1976).
  8. Forbes BA., et al. “Bailey and Scott’s Diagnostic Microbiology”. 10th St. Louis, Misssouri, USA: Mosby Inc.; (1998).
  9. Clinical and Laboratory Standard Institute. Performance Standards for Antimicrobial Susceptibility Testing. M2 A9. Pennsylvania, USA: Clinical and Laboratory Standard Institute 1.1 (2007).
  10. Malik S., et al. “Antibiogram of aerobic bacterial isolates from post- operative wound infections at a tertiary care hospital in India”. Journal of Infectious Diseases Antimicrobial Agents 28 (2011): 45-51.
  11. Satyanarayana V., et al. “Study of surgical site infections in abdominal surgeries”. Journal of Clinical and Diagnostic Research 5 (2011): 935-39.
  12. Lilani SP., et al. “Surgical site infection in clean and clean-contaminated cases”. Indian Journal of Medical Microbiology 23 (2005): 249-252.
  13. Khan AKA., et al. “A Study on the Usage Pattern of antimicrobial agents for the prevention of surgical site infections (ssis) in a tertiary care teaching hospital”. Journal of Clinical and Diagnostic Research 4 (2013): 671-674.
  14. Chakarborty SP., et al. “Isolation and identification of vancomycin resistant Staphylococcus aureus from postoperative pus sample”. Al Ameen Journal of Medical Sciences AJMS 2 (2011): 152-168.
  15. Kownhar H., et al. “High isolation rate of Staphylococcus aureus from surgical site infections in an Indian hospital”. Journal of Antimicrobial Chemotherapy 61 (2008): 758-760.
  16. Isibor OJ., et al. “Incidence of aerobic bacteria and Candida albicans in postoperative wound infections”. African Journal of Microbiology Research 2 (2008): 288-291.
  17. Anguzu JR and Olila D. “Drug sensitivity patterns of bacterial isolates from septic post-operative wounds in a regional referral hospital in Uganda”. African Health Science 3 (2007): 148-154.
  18. Aggarwal A., et al. “Correlation of beta-lactamase production/methicillin resistance and phage pattern of Staphylococcus aureus”. Indian Journal of Medical Sciences5 (2001): 253-256.
  19. Eagye KJ., et al. “Surgical site infections: does inadequate antibiotic therapy affect patient outcomes?” Surgery Infectious (Larchmt). 10.4 (2009): 323-331.
  20. Kaye KS., et al. “The effect of surgical site infection on older operative patients”. Journal of the American Geriatrics Society 1 (2009): 46-54.
  21. Sohn AH., et al. “Prevalence of surgical-site infections and patterns of antimicrobial use in a large tertiary-care hospital in Ho Chi Minh City, Vietnam”. Infection Control and Hospital Epidemiology 7 (2002): 382-387.
  22. Fletcher N., et al. “Prevention of perioperative infection”. Journal of Bone and Joint Surgery 7 (2007): 1605-1618.


Citation: Syeda Tamanna Tanjil., et al. “Microbiological Pattern of Surgical Site Infection Following Surgery at A Tertiary Level Hospital". Acta Scientific Microbiology 6.6 (2023): 05-10.


Copyright: © 2023 Syeda Tamanna Tanjil., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Acceptance rate30%
Acceptance to publication20-30 days

Indexed In

News and Events

  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is April 30th, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue".
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US