Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 6 Issue 5

Qualitative Determination of Amylase, Lipase, and Protease Production from Microorganisms Causing Deterioration in Raw and Processed Beef

Eze MC 1, Ogbonna CIC1, Ogbonna AI1, Eze C2 and Odenigbo EC1

1Department of Plant Science and Biotechnology, University of Jos, Nigeria

2Murtala Muhammed Specialist Hospital, Kano, Nigeria

*Corresponding Author: Eze MC, Department of Plant Science and Biotechnology, University of Jos, Nigeria.

Received: March 20, 2023; Published: April 18, 2023


Meat deterioration is a global problem and one of the leading causes of food wastage and substantial economic loss. Although several factors can accelerate this process, microorganisms are perhaps its main promoters due to intracellular enzymes released from lysed microbial cells during metabolism. The present study investigates the microorganisms isolated from meat samples that are responsible for the deterioration of the sample. The assay was carried out in triplicate. Pure cultures of bacteria and fungi used for amylase, lipase, and protease production were previously isolated from raw and processed meat samples. Sterile Bushnell-Hass medium containing 1% starch, olive oil, and skim milk in separate Petri dishes was used and thereafter the basal medium after incubation were flooded with Iodine, Sudan III and biuret solution respectively according to the enzyme assayed. The zones of clearing which were measured and analyzed using one-way ANOVA indicates amylase, lipase or protease secretion. Among the 20 isolates used for the assay, Klebsiella pneumoniae was the only bacteria isolate that showed clear zones (mm) for the amylase (4.67 mm), lipase (2.33 mm), and protease (6.33 mm). Other bacterial isolates showed clear zones only for either one or two of the enzymes. There was statistical significant different on the clear hydrolytic zones recorded for the bacterial isolates. The fungal isolates, Fusarium oxysporum, Mucor pusillus, and Alternaria alternata showed hydrolytic clear zones (mm) in all the three enzymes assayed signifying that these organisms were a contributory factor to the deterioration of the beef sample.

Keywords: Deterioration; Microorganism; Amylase; Lipase; Protease; Zones of Clearing


  1. Ogbeide AO. “Meat Industry Development in Nigeria: Implications of the Consumers’ Perspective”. Mayfair Journal of Agribusiness Management1 (2015): 59-75.
  2. Erkmen Osman and T Faruk Bozoglu. “Food microbiology”. 2-volume set: Principles into practice. John Wiley and Sons, (2016).
  3. Jay James M and James M Jay. "Intrinsic and extrinsic parameters of foods that affect microbial growth”. Modern Food Microbiology (1995): 38-66.
  4. Russell, et al. "Spoilage bacteria of fresh broiler chicken carcasses”. Poultry Science74.12 (1995): 2041-2047.
  5. Dave D and Ghaly AE. “Meat Spoilage Mechanisms and Preservation Techniques: A Critical Review”. American Journal of Agricultural and B:iological Sciences4 (2011) 486-510.
  6. Elbashir S., et al. "Seafood pathogens and information on antimicrobial resistance: a review”. Food Microbiology 70 (2018): 85-93.
  7. Eze , et al. "University of Jos, Isolation and identification of microorganisms from meat samples obtained from different areas in Jos metropolis”. Proceedings of the 47th Annual Conference of Animal Science Association of Nigeria (2022): 843-847.
  8. Eggins H O W and G JF Pugh. "Isolation of cellulose-decomposing fungi from the soil”. Nature4810 (1962): 94-95.
  9. Desnuelle P. "19 The Lipases”. The enzymes. Academic Press 7 (1972): 575-616.
  10. Oseni O A and M M Ekperigin. "Isolation and activity of alpha amylase from selected bacteria strains in the forest soil”. GJBB1 (2013): 17-18.
  11. Jadhav, et al. "Isolation and Identification of alpha amylase producing psychrotrophic Enterobacter sp. NACASA2”. Trends in Biotechnology Research 5.1 (2016): 9-17.
  12. Mitra, et al. "Alkaline lipase production from Enterobacter aerogenes by solid-state fermentation of agro-industrial wastes”. International Journal of Environment and Waste Management 5.3-4 (2010): 410-418.
  13. Bunn W Jason., et al. "Wall-associated processing of extracellular enzymes of Staphylococcus simulans biovar staphylolyticus”. FEMS Microbiology Letters1 (1998): 123-127.
  14. Pathak Sonal Sareen., et al. "Study of effect of temperature on amylase production by soil mycotic flora of Jabalpur region”. World Journal of Pharmacy and Pharmaceutical Sciences9 (2014): 1448-1458.
  15. Verma Poonam and R K Verma. "Qualitative estimation of amylase enzyme activity of fungal species isolated from iron ore mined overburden soil”. Tropical Plant Research3 (2018): 396-404.
  16. Yadav R P., et al. "Lipase production by Aspergillus and Penicillium species”. Folia Microbiologica 43 (1998): 373-378.
  17. Ozturkoglu-Budak, et al. "Protease and lipase activities of fungal and bacterial strains derived from an artisanal raw ewe's milk cheese”. International Journal of Food Microbiology 237 (2016): 17-27.
  18. Alves Maria Helena., et al. "Detection of extracellular protease in Mucor species”. Revista Iberoamericana de Micologia2 (2005): 114.
  19. Geoffry Kiptoo and Rajeshwara N Achur. "Screening and production of lipase from fungal organisms”. Biocatalysis and Agricultural Biotechnology 14 (2018): 241-253.


Citation: Eze MC., et al. “Qualitative Determination of Amylase, Lipase, and Protease Production from Microorganisms Causing Deterioration in Raw and Processed Beef". Acta Scientific Microbiology 6.5 (2023): 56-60.


Copyright: © 2023 Eze MC., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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