Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 6 Issue 4

Antimicrobial Effect of 1,25 Dihydroxy Vitamin D on Escherichia coli and its Association with Serum 25 Hydroxy Vitamin D Level: An Experimental Study on the Elderly Women

Joyeta Ghosh1*, Aditi Nag Chaudhuri2, Indranil Saha3 and Debnath Chaudhuri4

1Department of Dietetics and Applied Nutrition, Amity Institute of Applied Sciences (AIAS), Amity University - Kolkata Campus, Major Arterial Road, Action Area II, Kadampukur Village, Rajarhat, Newtown, Kolkata, West Bengal 700135, India.

2Department of Microbiology, Lady Brabourne College, University of Calcutta, Kolkata, India

3ICMR-Centre for Ageing & Mental Health, Indian Council of Medical Research, Salt Lake, Kolkata, West Bengal, India

4Department of Biochemistry and Nutrition, All India Institute of Hygiene & Public Health, Kolkata, West Bengal, India

*Corresponding Author: Joyeta Ghosh, Department of Dietetics and Applied Nutrition, Amity Institute of Applied Sciences (AIAS), Amity University - Kolkata Campus, Major Arterial Road, Action Area II, Kadampukur Village, Rajarhat, Newtown, Kolkata, West Bengal 700135, India.

Received: February 06, 2023; Published: March 13, 2023


Introduction: Vitamin D plays crucial role as an antimicrobial agent, the deficiency of which has deleterious effects on the general wellbeing, especially among elderly. The incidence and severity of infectious diseases in the elderly are associated with a variety of functional, demographic and immunologic changes inside the body due to ageing.

Objective: The aim was to examine the association of vitamin D status with antimicrobial activity of cultured macrophages isolated within an exclusively elderly population cohort. In addition, the present study was also determined the antimicrobial activity (against E. coli infection) of cultured macrophages after in vitro supplementation of Vitamin D.

Materials and Methods: This experimental study was conducted among 97 randomly selected rural elderly women aged between 60 to 70 years of age, during the period of April 2014 to August 2018,at Amdanga block, North 24th parganas, West Bengal. Their vitamin D status was assessed by the estimation of serum 25(OH)D and classified into three groups viz. sufficient (40 members), insufficient (28 members) and deficient (29 members). After that the Peripheral Blood Mononuclear Cells (PBMC) were isolated and cultured from fresh blood from each and every study subject. Supplementation of 1,25 dihydroxy vitamin D[1,25(OH)2D] was given selectively at a dose of 10 ×10-8 M for 72 hours in the culture media and were exposed to Escherichia coli and screened for their iNOS activity (inducible Nitric Oxide Sythase), SOD activity (Superoxide Dismutase) and CFU (Colony Forming Unit) reduction rate. SPSS software, version 20.0 was used to perform statistical analysis.

Results: iNOS activity and SOD activity were significantly increased in case of both sufficient and deficient group. As per the CFU reduction rate against E. coli infection there is no significant difference were observed according to serum 25(OH)D consisting group. After in vitro 1,25(OH)2D supplementation,the maximum increase in CFU reduction rate was observed among Deficient group(63.57%), whereas in case of Insufficient group it was 60.11% and for Sufficient group it was 44.66%.

Conclusion: Considering bacteria killing capacity of macrophages the in vitro 1,25(OH)2D supplementation significantly inreases the CFU reduction rateoverall. Sufficient group’s macrophages always had better profile than other two groups. In vitro 1,25(OH)2D supplementation increases iNOS and SOD activity significantly.

 Keywords: E. coli; iNOS Activity; SOD Activity; Bacteria Killing Assay; Elderly Women


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Citation: Joyeta Ghosh., et al. “Antimicrobial Effect of 1,25 Dihydroxy Vitamin D on Escherichia coli and its Association with Serum 25 Hydroxy Vitamin D Level: An Experimental Study on the Elderly Women". Acta Scientific Microbiology 6.4 (2023): 27-34.


Copyright: © 2022 Joyeta Ghosh., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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