Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 6 Issue 4

Toxicity Of Dithiothreitol (DTT) Onlipolytic Activity of Maruca vitrata (Fabricius)

SA Jadhav1* and RM Gejage2

1Research Student, Department of Zoology, Y.C. College of Science, India

2Professor and Head, Department of Zoology, Smt. K.R.P. Kanya Mahavidyalaya, Islampur, India

*Corresponding Author: SA Jadhav, Research Student, Department of Zoology, Y.C. College of Science, India.

Received: February 21, 2023; Published: March 06, 2023

Abstract

Losses of agriculture product because of pest have been evaluated worldwide. The partial characterization of larval lipase of Maruca vitrata (Fabricius) shows higher activity at pH 7.9 and Km 9.56×10-2 mM. The gradual increase in lipase activity was observed from 4 to 8-day larvae, decrease from 8 to 13th day and maximum activity in 8-day in control and dithiothreitol treated larvae of M. vitrata. The lipase activity of 9-day control larvae is 0.280 and dithiothreitol treated larvae is 0.274 µmol FFA/mg protein/25 min. The lipase activity of 8-day control larvae was 1.02 fold more than dithiothreitol treated larvae. The inhibitory role of dithiothreitol on larval lipaseMaruca vitrata has been attempted in present work.

 Keywords: Lipase, larva, Dithiothreitol (DTT), M. vitrata (F).

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Citation

Citation: SA Jadhav and RM Gejage. “Toxicity Of Dithiothreitol (DTT) Onlipolytic Activity of Maruca vitrata (Fabricius). Acta Scientific Microbiology 6.4 (2023): 10-12.

Copyright

Copyright: © 2022 SA Jadhav and RM Gejage. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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