SA Jadhav¹* and RM Gejage²

¹Research Student, Department of Zoology, Y.C. College of Science, India

*Corresponding Author: SA Jadhav, Research Student, Department of Zoology, Y.C. College of Science, India.

Received: February 21, 2023; Published: March 06, 2023

Abstract

Losses of agriculture product because of pest have been evaluated worldwide. The partial characterization of larval lipase of Maruca vitrata (Fabricius) shows higher activity at pH 7.9 and K_m 9.56×10^-2 mM. The gradual increase in lipase activity was observed from 4 to 8-day larvae, decrease from 8 to 13^th day and maximum activity in 8-day in control and dithiothreitol treated larvae of M. vitrata. The lipase activity of 9-day control larvae is 0.280 and dithiothreitol treated larvae is 0.274 µmol FFA/mg protein/25 min. The lipase activity of 8-day control larvae was 1.02 fold more than dithiothreitol treated larvae. The inhibitory role of dithiothreitol on larval lipaseMaruca vitrata has been attempted in present work.

 Keywords: Lipase, larva, Dithiothreitol (DTT), M. vitrata (F).

References

1. Shanower TG., et al. “Insect pests of pigeon pea and their management”. Annual Review of Entomology 44 (1999): 77-96
2. Vishakantaiah M and Jagadeesh Babu CC. “Bionomics of the tur web worm, Maruca testualis (Lepidoptera: Pyralidae)”. Mysore Journal of Agricultural Sciences 14 (1980): 52-53.
3. Abdullahi M M and Ibrahim S K. “Assessment of the effect of time, synthetic and botanical insecticides spray regimes on the abundance of predators in cowpea production in Kebbi State of Nigeria”. Journal of Agriculture and Veterinary Sciences 7.5 (2014): 87-95.
4. Naumff DG. “Β- fructosidase superfamily: Homology with some α-L-arabinases and β—D-xylosidases”. PROTEINS : Structure, Function, and Bioinformatics 42 (2007): 66-76.
5. Grillo LAM., et al. “Lipid metabolism in Rhodnius prolixus (Hemiptera: Reduviidae): role of a midgut triacylglycerol-lipase”. Insect Biochemistry and Molecular Biology6 (2007): 579-588.
6. Wang Y., et al. “Toxicity of Dithiothreitol (DTT) to Drosophila melanogaster”. Toxicology Report 8 (2021): 124-130.
7. Sharma HC. “Bionomics, host plant resistance, and management of the legume pod borer, Maruca vitrata - a review”. Crop Protection5 (1998): 173-386.
8. Santana CC., et al. “Embryonic development of Rhynchophorus palmarum (Coleoptera: Curculionidae): Dynamics of energy source utilization”. Journal of Insect Science 14 (2014): 280-289.
9. Hayase K and TappleA L. “Specificity and other properties of lysosomal lipase of rat liver”. Journal of Biological Chemistry 245 (1970): 169-175.
10. Itaya KA. “More sensitive and stable colorimetric determination of free fatty acids in blood”. Journal of Lipid Research 18 (1977): 663-665.
11. Dawson R MC. “In Data for Biochemical Research”. 2^nd Oxford University press, Oxford (1969): 616.
12. Hiol A., et al. “Purification and characterization of an extracellular lipase from a thermophilic Rhizopus oryzae strain isolated from palm fruit”. Enzyme and Microbial Technology5-6 (2000): 421-430.
13. Sharma R., et al. “Production, purification, characterization, and applications of lipases”. Biotechnology Advances 8 (2001): 627-662.
14. Mrdakovic M., et al. “Partialcharacterization of lipase from Gypsy Moth (Lymantria disper) larval midgut”. Folia Biologica (Krakow) 56 (2008): 103-110.

Citation

Citation: SA Jadhav and RM Gejage. “Toxicity Of Dithiothreitol (DTT) Onlipolytic Activity of Maruca vitrata (Fabricius). Acta Scientific Microbiology 6.4 (2023): 10-12.

Copyright

Copyright: © 2022 SA Jadhav and RM Gejage. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.