Kwok Ming Wong, Bryan JH Sim and Maurice HT Ling*

School of Applied Sciences, Temasek Polytechnic, Singapore

*Corresponding Author: Maurice HT Ling, School of Applied Sciences, Temasek Polytechnic, Singapore.

Received: January 27, 2023; Published: February 15, 2023

Abstract

Saccharomyces cerevisiae is an important experimental organism for industrial and scientific research with S. cerevisiae S288C as the first eukaryote genome sequenced. Genome-scale metabolic models (GSMs) are computational tools to explore metabolic engineering requirements. Currently, there are 2 major GSMs of S. cerevisiae S288C, iND750 and iMM904, which raises the question of whether they are consistent to each other. Here, we compare iND750 and iMM904 by examining the fluxomic changes resulting from single reaction knockouts. 40.5% to 50.3% (n = 637) of the reactions are common in both GSMs. Of which, 64 (10.0% of common reactions, or between 4.1% and 5.2% of the total reactions in each GSM) reaction knockouts resulted in significant fluxomic changes. This is significantly lower (t = -15.882, df = 30, p-value = 3.82E-16) from expected using randomization test, suggesting that iND750 and iMM904 are likely to be consistent with each other from the perspective of common reactions.

Keywords: Saccharomyces cerevisiae; Genome-scale Metabolic Models (GSMs);

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Citation

Citation: Maurice HT Ling., et al. “Consistency between Saccharomyces cerevisiae S288C Genome Scale Models (iND750 and iMM904)". Acta Scientific Microbiology 6.3 (2023): 63-68.

Copyright

Copyright: © 2022 Maurice HT Ling., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.