Benibo N*, Obire O, Douglas SI and Nrior RR

Department of Microbiology, Faculty of Science, Rivers State University, Nkpolu-Oroworukwo, Port Harcourt, Nigeria

*Corresponding Author: Benibo N, Department of Microbiology, Faculty of Science, Rivers State University, Nkpolu-Oroworukwo, Port Harcourt, Nigeria.

Received: January 5, 2023; Published: February 10, 2023

Abstract

The presence of Polycyclic Aromatic Hydrocarbons (PAHs) in territorial water is of great concern due to the carcinogenicity. In this study, monthly collection of surface water from four artisanal petroleum marketing points was made from November 2019 - October 2020 using standard methods. Hydrocarbon utilizing (Hydrocarbonoclastic) bacteria were isolated by vapour phase transfer method using mineral salts medium, isolates were identified based on cultural, morphological and biochemical characteristics. The mean HUB count was 1.2 x 10⁴ cfuml^-1. The HUB isolates were Pseudomonas, 12.4%; Chromobacterium, 1.8%; Serratia, 5.3%; Corynebacterium, 6.25%; Escherichia, 12.1%; Bacillus, 12.4%; Staphylococcus, 10.6%; Micrococcus, 3.5%; Citrobacter, 7.1%; Enterobacter, 3.5%; Acinetobacter, 3.5%; Alcaligens, 4.4%; Nocardia, 3.5%; Streptococcus, 6.2%; and Shigella. 6.2%. The PAHs level in the surface water samples were determined used Gas Chromatography - Flame Ionization Detector, GC-FID. The PAHs level observed was a mean of 0.004mgl^-1 which is below the EU permissible limit of 0.007mgl^-1; which can be attributed to activities of the polycyclic aromatic hydrocarbon mineralizing bacteria present as a natural consortium in the ecosystem. If the presence of these strains of bacteria continues in the ecosystem, the continual stability of the ecosystem can be ensured. This study reveals a natural bacterial consortium that can degrade PAH in the surface water within a short time.

Keyword: Wetland; Crude Oil Pollution; Hydrocarbonclastic Bacteria; PAHs; Borikiri

References

1. Moslen, M and Miebaka CA. “Hydrocarbon contamination of sediments in the Niger Delta Region: a case study of the Azuabie creek, upper reaches of the Bonny Estuary, Nigeria”. IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) 11 (2017): 26-32.
2. Ogbonna DN., et al. “Evaluation of Organic Nutrient Supplements and Bioaugmenting Microorganisms on Crude Oil Polluted Soils”. Current Journal of Applied Science and Technology6 (2019): 1-19.
3. Ule O., et al. “Myco-enhanced Bioremediation in Open Field Crude Oil Contaminated Soil Using Mucor racemosus and Aspergillus niger”. Current Journal of Applied Science and Technology1 (2021): 119-141.
4. Oboh BO., et al. “Hydrocarbon Degrading Potentials of bacteria isolated from a Nigeria Bitumen (Tarsand) deposit”. Nature Science 4 (2006): 51-57.
5. Temitayo OO., et al. “Degradation of polyaromatic fractions of crude oil and detection of catabolic genes in hydrocarbon-degrading bacteria isolated from Agbabu bitumen sediments in Ondo State”. AIMS Microbiology4 (2019): 308-323.
6. Makinde OO., et al. “Comparative Assessment of Physical and Chemical characteristics is Ekerekana and Buguma Creeks, Niger Delta Nigeria”. Journal of Environmental Protection and Sustainable Development3 (2015): 126-133.
7. Eke IB and Sikoki FD. “The state and seasonal variability of some physic-chemical parameters in the new Calabar River, Nigeria”. Supp and Acta Hydrobia 5 (2003): 45-60.
8. “Official methods of Analysis of the Association of the Official Analytical Chemists”. 7^th edition AOAC International, Washington, DC, USA”. (2000).
9. “Standard method for the examination of water and waste water”. American Public Health Association, Washington USA. (1999).
10. Obire O and Amusan IO. “The environmental impact of oil field “Formation water" in a freshwater stream in Nigeria”. Journal of Applied Science Environment Management1 (2013): 61-66.
11. Cheesbrough, M. “District Laboratory Practice in tropical countries (Part II)”. Cambridge, University Press UK. (2000): 134-143.
12. Nrior RR. “Ecotoxicological Assessment of Nigeria Locally Refined Diesel and Kerosene on Aspergillus niger a Key Fungal Pollution Biomarker”. Asian Journal of Biology4 (2018): 1-8.
13. Holt JG., et al. “Bergey’s Manual of Determinative Bacteriology”. Williams and Wilkins, Baltimore, Maryland, USA (1994): 151-157.
14. Chikere, CB., et al. “Characterization of hydrocarbon utilizing bacteria in tropical marine sediments”. African Journal of Biotechnology11 (2009): 2541-2544.
15. Ariyo AB and Obire O. “Microbiological and Physicochemical Characteristics of Abattoir Wastewaters in Bayelsa and Rivers State”. South Asian Journal of Research in Microbiology (SAJRM)1 (2021): 32-45.
16. Douglas SI., et al. “Effects of Three Organic Amendments on Polycyclic Aromatic Hydrocarbon Degradation from Crude Oil Polluted Artisanal Refining Site”. International Journal of Current Microbiology and Applied Sciences12 (2020): 488-502.
17. Edlund A and Jansson JK. “Changes in active bacterial communities before and after dredging of a highly polluted Baltic Sea sediments”. Applied and Environmental Microbiology 72 (2006): 6800-6807.
18. Varjani SJ and Upasaani VN. "Biodegradation of petroleum hydrocarbons by an oleophilic strain of Pseudomonas aeruginosa NCIM 5514”.  Bioresource Technology 222 (2016): 195-202.
19. Daka ER and Chinedu OA. “Monitoring of surface water quality during the construction of Riverbank protection at a section of the Nun River in the Niger Delta, Nigeria”. African Journal of Environmental Pollution and Health 10 (2013): 23-29.
20. Ariyo AB and OBIRE O. “Polycyclic aromatic hydrocarbons (PAHs) and Heavy metal content of abattoir Wastewaters in Bayelsa and Rivers State”. International Journal of Scientific and Management Research5 (2022): 25-33.
21. Adeyemo OK. “Consequences of pollution and degradation of Nigerian aquatic environment on fisheries resources”. The Environmentalist 23 (2003): 297-306.

Citation

Citation: Benibo N., et al. “Hydrocarbonoclastic Bacteria and Polycyclic Aromatic Hydrocarbon Profile of Surface Water in Borikiri Wetlands, Port Harcourt, Nigeria". Acta Scientific Microbiology 6.3 (2023): 41-45.

Copyright

Copyright: © 2022 Benibo N., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.