Biofilm Formation in Methicillin Resistant Staphylococci aureus at the University of Ilorin Teaching Hospital, Ilorin
Oshin Oluwaseyi Babatunde*, Adejuyigbe Zacchaeus and Prof Charles Nwabuisi
Department of Medical Microbiology, University of Ilorin, Nigeria
*Corresponding Author: Oshin Oluwaseyi Babatunde, Department of Medical Microbiology, University of Ilorin, Nigeria.
November 03, 2022; Published: January 24, 2023
Biofilm is a structural community of bacterial cells enclosed in a self-produced polymeric matrix which could adhere to inert or living surfaces. It has also been discovered that 99.0% of bacteria exist in this community, with only 1.0% living in planktonic state and that 65.0% of microbial infections are associated with biofilms. Microorganisms that grow within the biofilm state possess several mechanisms that increase resistance to external antimicrobial treatments. The objective of the study is to determine the prevalence of biofilm forming ability amongst Methicillin resistance Staphylococci aureus at the University of Ilorin Teaching Hospital. One hundred and sixty eight (168) staphylococcal isolates from different clinical specimens were collected from the Microbiology Laboratory of University of Ilorin Teaching Hospital (UITH). The isolates were collected into 20% glycerol-brain heart infusion broth in vials and stored at -20oC for further processing. The isolates were re-characterized using standard microbiological techniques. Biofilm detection and quantification was carried out using modified Christensen’s Microtitre plate method and the optical density determined at 450 nm.
The prevalence of biofilm formation among Staphylococcal isolates was 56.5%. Staphylococcal isolates showed moderate resistance to almost all the antibiotics (Gentamycin, Erythromycin, Tetracycline, Ciprofloxacin, Amoxicillin-Clavulanic acid, Cefuroxime and Linezolid) with Tetracycline (51.8%), Ciprofloxacin (42.3%), and Gentamycin (35.7%) with the highest resistance. Biofilm producers and non-biofilm producers exhibited 100% sensitivity to Linezolid. The prevalence of methicillin resistance among staphylococcal isolates was 44.6. There was a significant difference (P < 0.05) in the distribution of biofilm production among Staphylococcal isolates.
The prevalence of biofilm production at UITH is relatively high (56.5%) and of grave concern considering the devastating effect of antimicrobial resistance. Linezolid still remain a drug of choice in managing Staphylococcal infections and also infections caused by methicillin resistant staphylococci. And there is a need to include biofilm detection protocol in the routine microbiological examination with an objective to curbing antimicrobial resistance.
Keywords: Biofilm Formation; Methicillin Resistance; S. aureus; Northern Nigeria; Health Facilities
- Costerton J., et al. “Bacterial Biofilm: A common cause of persistent Infections”. Science 284 (1999): 1318-1322.
- Kreth J and Herzberg M. “Molecular Principles of Adhesion and Biofilm Formation”. In: L.E. Cha ´vez de Paz et al. (eds.), The Root Canal Biofilm, (2015): 23-53.
- Pamp S., et al. “Insight into the Microbial Multicellular Lifestyle via Flow-Cell Technology and Confocal Microscopy”. Cytometry 75 (2009): 90-103.
- Potera C. “Biofilms invade Microbiology”. Science 5283 (1996): 1795-1797.
- Maric S and Vrane J. “Characteristics and Significance of Microbial Biofilm Formation, 2007”. Periodicum Biologorum2 (2007): 1-5.
- Mittelman M. “Adhesion to Biomaterials”. In: Fletcher M (ed) Bacterial Adhesion: Molecular and Ecological Diversity. Wiley-Liss, New York, (1996): 89-127.
- Arciola C., et al. “Polysaccharide Intercellular Adhesin in Biofilm: Structural and Regulatory Aspects”. Frontiers in Cellular and Infection Microbiology 5 (2015): 1-10.
- Sahra Kırmusaoğlu. “Staphylococcal Biofilms: Pathogenicity, Mechanism and Regulation of Biofilm Formation by Quorum-Sensing System and Antibiotic Resistance Mechanisms of Biofilm-Embedded Microorganisms”. In: Dharumadurai Dhanasekaran and Nooruddin Thajuddin (eds) Microbial Biofilms - Importance and Applications. IntechOpen Limited, UK (2016): 190-209.
- Speziale P., et al. “Protein-based Biofilm matrices in Staphylococci”. Frontiers in Cellular and Infection Microbiology 4 (2014): 171.
- O’Neill E., et al. “A novel Staphylococcus aureus Biofilm Phenotype mediated by the Fibronectin-Binding Proteins, FnBPA and FnBPB”. Journal of Bacteriology 190 (2008): 3835-3850.
- Foster T., et al. “Adhesion, Invasion and Evasion: The many Functions of the Surface Proteins of Staphylococcus aureus”. Nature Reviews Microbiology 12 (2014): 49-62.
- Tille P. “Bailey and Scott’s Diagnostic Microbiology”. Elsevier Mosby, USA, thirteenth Edition. (2014): 232-244.
- Brooks G., et al. “Jawetz Melnick and Adelberg’s Medical Microbiology”. 26th edition, McGraw Hill Medical Companies (2013): 199-206.
- Monica Cheesbrough. “District Laboratory Practical in Tropical Countries”. Cambridge University Press, Edinburg building, Trumpington street, Cambridge CB2 1IR, United Kingdom (2006).
- Christensen G., et al. “Adherence of Coagulase Negative Staphylococci to Plastic Tissue Culture Plates: A Quantitative Model for the Adherence of Staphylococci to Medical Devices”. Journal of Clinical Microbiology 22 (1985): 996-1006.
- Stepanovic S., et al. “Influence of Dynamic Conditions on Biofilm Formation by Staphylococci”. European Journal of Clinical Microbiology and Infectious Diseases 20 (2001): 502-504.
- Merritt J., et al. “Growing and Analyzing Static Biofilms, current”. Protocols in Microbiology 22 (2011): 1B.1.1-1B.1.18.
- Hassan A., et al. “Evaluation of Different Detection Methods of Biofilm Formation in the Clinical Isolates”. Brazilian Journal of Infectious Diseases4 (2011): 305-311.
- Chai F., et al. “Microtitre Plate Assay for the Quantification of Biofilm Formation by Pathogenic Leptospira”. Research Journal of Microbiology 12 (2017): 146-153.
- CLSI Performance Standards for Antimicrobial Susceptibility Testing. 28th CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute (2018).
- Nwoire A., et al. “Incidence of Staphylococcus aureus in Clinical Specimens in Federal Teaching Hospital, Abakalike, Ebonyi, Merit”. Research Journal of Medicine and Medical Sciences3 (2013): 043-046.
- Abirami L., et al. “Biofilm Formation and Methicillin Resistance among the Staphylococcus aureus causing Burn Wound Infections in a Tertiary Care Hospital: A Comparative Study of the Antibiotic Susceptibility Pattern between Biofilm Producers and Non-biofilm Producers”. Journal of Pharmaceutical and Biomedical Sciences 3 (2016): 179-183.
- Eyoh A., et al. “Relationship between Multiple Drug Resistance and Biofilm Formation in Staphylococcus aureus Isolated from Medical and Non-Medical Personnelin Yaounde, Cameroun”. Pan African Medical Journal186 (2014): 1-11.
- Chibueze I., et al. “Biofilm Formation and Antibiotic Susceptibility Profile of Clinical Isolates of Staphylococcus aureus Isolated from Clinical Samples in Zaria, Nigeria”. Clinical Microbiology4 (2017): 1-11.
- Walker J., et al. “Catherization alters Bladder ecology to Potentiate Staphylococcus aureus Infection of the Urinary Tract”. Proceedings of the National Academy of Sciences of the United States of America41 (2017): E8721-E8730.
- Kawamura H., et al. “Quantitative Analysis of Biofilm Formation of Meticillin -Resistant Staphylococcus aureus (MRSA) strains from Patients with Orthopaedic Device- related Infections, Federation of European Micobioogical Societies”. Medical Microbiology and Immunology 63 (2011): 10-15.
- Gad G., et al. “Detection of icaA, icaD genes and Biofilm Production by Staphylococcus aureus and Staphylococcus epidermidis Isolated from Urinary Tract Catherized Patients”. Journal of Infection in Developing Countries5 (2009): 342-351.
- Abdel Halim R., et al. “Detection of Biofilm Producing Staphylococci among Different Clinical Isolates and Its Relation to Methicillin Susceptibility”. Open Access Macedonian Journal of Medical Sciences8 (2018): 1335-1341.
- Filho R., et al. “Biofilm Production Clinical Isolates of Staphylococcus epidermidis and its Relationship with Genetypic Profile, Presence of Virulence- Related Genes and Antibiotic Resistance”. African Journal of Microbiology Research14 (2015): 1026-1036.
- Sauer K., et al. “Pseudomonas aeruginosa displays Multiple Phenotypes during Development as a biofilm”. Journal of Bacteriology 184 (2002): 1140-1154.
- Anderson G and O’Toole A. “Innate and Induced Resistance Mechanisms of Bacterial Biofilms”. In: Romeo, T. (eds.) Bacterial Biofilms. Current Topics in Microbiology and Immunology 322, Springer-Verlag: Berlin Heidelberg 322 (2008): 85-105.
- Samie A and Shivambu N. “Biofilm Production and Antibiotic Susceptibility Profiles of Staphylococcus aureus Isolated from HIV and AIDS Patients in the Limpopo Province, South Africa”. African Journal of Biotechnology 65 (2011): 14625-14636.
- Chika E., et al. “Detection of constitutive and inducible-clindamycin-resistance in clinical isolates of Staphylococcus aureus from a Federal Teaching Hospital in Abakaliki, Nigeria”. Journal of Bacteriology and Infectious Disease 1 (2011): 31-34.