Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 6 Issue 2

Biofilm Formation in Methicillin Resistant Staphylococci aureus at the University of Ilorin Teaching Hospital, Ilorin

Oshin Oluwaseyi Babatunde*, Adejuyigbe Zacchaeus and Prof Charles Nwabuisi

Department of Medical Microbiology, University of Ilorin, Nigeria

*Corresponding Author: Oshin Oluwaseyi Babatunde, Department of Medical Microbiology, University of Ilorin, Nigeria.

Received: November 03, 2022; Published: January 24, 2023

Abstract

Biofilm is a structural community of bacterial cells enclosed in a self-produced polymeric matrix which could adhere to inert or living surfaces. It has also been discovered that 99.0% of bacteria exist in this community, with only 1.0% living in planktonic state and that 65.0% of microbial infections are associated with biofilms. Microorganisms that grow within the biofilm state possess several mechanisms that increase resistance to external antimicrobial treatments. The objective of the study is to determine the prevalence of biofilm forming ability amongst Methicillin resistance Staphylococci aureus at the University of Ilorin Teaching Hospital. One hundred and sixty eight (168) staphylococcal isolates from different clinical specimens were collected from the Microbiology Laboratory of University of Ilorin Teaching Hospital (UITH). The isolates were collected into 20% glycerol-brain heart infusion broth in vials and stored at -20oC for further processing. The isolates were re-characterized using standard microbiological techniques. Biofilm detection and quantification was carried out using modified Christensen’s Microtitre plate method and the optical density determined at 450 nm.

The prevalence of biofilm formation among Staphylococcal isolates was 56.5%. Staphylococcal isolates showed moderate resistance to almost all the antibiotics (Gentamycin, Erythromycin, Tetracycline, Ciprofloxacin, Amoxicillin-Clavulanic acid, Cefuroxime and Linezolid) with Tetracycline (51.8%), Ciprofloxacin (42.3%), and Gentamycin (35.7%) with the highest resistance. Biofilm producers and non-biofilm producers exhibited 100% sensitivity to Linezolid. The prevalence of methicillin resistance among staphylococcal isolates was 44.6. There was a significant difference (P < 0.05) in the distribution of biofilm production among Staphylococcal isolates.

The prevalence of biofilm production at UITH is relatively high (56.5%) and of grave concern considering the devastating effect of antimicrobial resistance. Linezolid still remain a drug of choice in managing Staphylococcal infections and also infections caused by methicillin resistant staphylococci. And there is a need to include biofilm detection protocol in the routine microbiological examination with an objective to curbing antimicrobial resistance.

Keywords: Biofilm Formation; Methicillin Resistance; S. aureus; Northern Nigeria; Health Facilities

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Citation

Citation: Oshin Oluwaseyi Babatunde., et al. “Biofilm Formation in Methicillin Resistant Staphylococci aureus at the University of Ilorin Teaching Hospital, Ilorin". Acta Scientific Microbiology 6.2 (2023): 92-99.

Copyright

Copyright: © 2022 Oshin Oluwaseyi Babatunde., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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