Preliminary Screening of Biosurfactants Produced by a Pseudomonas sp. Isolated from Mud Drill Cuttings
Onwuka IB1, Ibiene AA1, Eresia-Eke R2, Dirisu CG3* and Nwogbidi K3
1Department of Microbiology, University of Port Harcourt, Nigeria
2Department of Biological Sciences, University of Calgary, Canada
3Department of Biology Education, Federal College of Education (Technical), Omoku, Nigeria
*Corresponding Author: Dirisu CG, Department of Biology Education, Federal College of Education (Technical), Omoku, Nigeria.
December 19, 2022; Published: January 13, 2023
Biosurfactants are a group of surface-active molecules synthesized by microorganisms. They either adhere to cell surfaces or are excreted extracellularly into the growth medium. A bacterial isolate identified as Pseudomonas sp. was cultured in a synthetic medium adjusted to varying acidic to alkaline conditions with varying concentrations of sodium chloride. Screening tests were carried out on the bacterial isolate to verify its ability to produce biosurfactants. The effect of the media on the emulsification index and surface tension of the free product showed that although the isolate was found to be osmotolerant, withstanding and growing in media containing high sodium chloride concentration, biosurfactant was produced optimally at pH 7.2 and sodium chloride (NaCl) concentration of 3%. The cell-free media recorded optimum emulsification index of 66.5% and reduced the surface tension of the culture media from 59.7 mN/m2 to 28.1 mN/m2.
Keywords: Biosurfactant; Drilling Mud; Emulsification Index; Pseudomonas sp
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