Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 6 Issue 2

Biodegradation of Phenol by Local Strain of Bacteria Isolated from Crude Oil Contaminated Soil

Otman Ermithi*, Abdurrezg Elfahem, Abdul Ghaffar Elosta, Saad Belaid, Ahmed Agha, Tarek Abukreba, Najah Errhel, Abdurraof Habibi, Khalid Naffati and Hatem Salem

Department of Microbiology, Biotechnology Research Center-Tripoli, Libya

*Corresponding Author: Otman Ermithi, Department of Microbiology, Biotechnology Research Center-Tripoli, Libya.

Received: October 21, 2022; Published: January 12, 2023

Abstract

Phenol is one of the major organic pollutant, which present in numerous industrial wastewater products, including oil refineries and petrochemical. Phenol can be removed by biological treatment using microorganisms which may solve this problem completely. In this study we sought to use some local bacterial strains to investigate their ability to treat industrial wastewater and remove phenolic compounds from it. This study was carried out using nine local bacterial strains isolated from crude oil contaminated soil from Alzawia oil refining company in Libya. The strains were cultivated in minimal salt medium with 200 mg/l phenol concentration as the sole carbon source. Bacterial growth was measured by the optical density at (OD600 nm) and phenol percentage. Among all strains used Pseudomonas aeruginosa was found the best strain has phenol removal 88% within 24h at37 C in the initial concentration of phenol (200 mg/l) and 97% within 48h. The local isolated strain can lead to a good application for bioremediation of pollutants phenolic compounds.

Keywords: Biodegradation; Phenol; Industrial Wastewater; Pseudomonas aeruginosa

References

  1. H Movahedyan and H Khorsandi. “Detection of depredation bacteria and Pseudomonas putida in activated by polymerase chain reaction”. Journal of Environmental Health Science and Engineering 6 (2009): 115-120.
  2. Bhavna V Mohite and Ruby E Jalgonwala. “Isolation and characterization of phenol degrading bacteria from oil contaminated soil”. Innovation Romanian Food Biotechnology 7 (2010).
  3. S Chakraboty and T Battacharya. “Biodegradation of phenol by native microorganisms isolated from coke processing wastewater”. Journal of Environment Biology (2010): 293-296.
  4. R Shawabkeh., et al. “Rate of biodegradtion of phenol by Klebsiella oxytoca in minimal medium and nutrient broth condition”. Bioremediation Journal1 (2007): 13-19.
  5. Mitra Shourian., et al. “Effect phenol degradation by a newly characterized Pseudomonas SA01 isolated from pharmaceutical waste water”. Desalination 246 (2009): 577-594.
  6. Wael S EL-Sayed and Mohamed K Ibrahim. “Isolation and characterization of phenol-catabolizing bacteria from coking plant”. Bioscience, Biotechnology, and Biochemistry 9 (2003): 2026-2029.

Citation

Citation: Otman Ermithi., et al. “Biodegradation of Phenol by Local Strain of Bacteria Isolated from Crude Oil Contaminated Soil". Acta Scientific Microbiology 6.2 (2023): 45-47.

Copyright

Copyright: © 2022 Otman Ermithi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days

Indexed In






News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is December 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"

Contact US