Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 6 Issue 2

Biodegradation of Phenol by Local Strain of Bacteria Isolated from Crude Oil Contaminated Soil

Otman Ermithi*, Abdurrezg Elfahem, Abdul Ghaffar Elosta, Saad Belaid, Ahmed Agha, Tarek Abukreba, Najah Errhel, Abdurraof Habibi, Khalid Naffati and Hatem Salem

Department of Microbiology, Biotechnology Research Center-Tripoli, Libya

*Corresponding Author: Otman Ermithi, Department of Microbiology, Biotechnology Research Center-Tripoli, Libya.

Received: October 21, 2022; Published: January 12, 2023


Phenol is one of the major organic pollutant, which present in numerous industrial wastewater products, including oil refineries and petrochemical. Phenol can be removed by biological treatment using microorganisms which may solve this problem completely. In this study we sought to use some local bacterial strains to investigate their ability to treat industrial wastewater and remove phenolic compounds from it. This study was carried out using nine local bacterial strains isolated from crude oil contaminated soil from Alzawia oil refining company in Libya. The strains were cultivated in minimal salt medium with 200 mg/l phenol concentration as the sole carbon source. Bacterial growth was measured by the optical density at (OD600 nm) and phenol percentage. Among all strains used Pseudomonas aeruginosa was found the best strain has phenol removal 88% within 24h at37 C in the initial concentration of phenol (200 mg/l) and 97% within 48h. The local isolated strain can lead to a good application for bioremediation of pollutants phenolic compounds.

Keywords: Biodegradation; Phenol; Industrial Wastewater; Pseudomonas aeruginosa


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Citation: Otman Ermithi., et al. “Biodegradation of Phenol by Local Strain of Bacteria Isolated from Crude Oil Contaminated Soil". Acta Scientific Microbiology 6.2 (2023): 45-47.


Copyright: © 2022 Otman Ermithi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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