Antimicrobial Susceptibility Testing and Biofilm Production of Burkholderia cepacia Complex Organisms from Ultrasound Gels in India
Ravichandran Karthikeyan, Bhoj R Singh*, Akanksha Yadav, Himani Agri, Varsha Jayakumar, Vinodhkumar OR, Dharmendra K Sinha
Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar, India
*Corresponding Author: Bhoj R Singh, Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar, India.
December 12, 2022; Published: January 06, 2023
Burkholderia cepacia complex (Bcc) bacteria are versatile difficult to treat pathogens
with extraordinary metabolic diversity. They are important nosocomial pathogens having multi-drug
resistant potential. Contamination and biofilm formation on medical devices and equipment are the
important reasons for the multiple outbreaks in recent decades. In this study, we subjected 32
isolates of Bcc from ultrasound gels to the two important virulent traits such as antimicrobial susceptibility testing
and biofilm producing ability with and without different stress conditions. All the isolates were susceptible
to co-trimoxazole (TMP-SMX) (100%) suggesting it as the preferred drug or the drug of the first
choice for treating Bcc infections. The isolates showed the varied degree of susceptibility to meropenem
(91%), doxycycline (85%), gatifloxacin (85%), piperacillin+ tazobactam (82%), ceftazidime (79%), and
levofloxacin (71%). Multiple antibiotic resistance (MAR) indices for all the isolates were >0.2
and three isolates had >0.5. Most of the Bcc isolates (81.25%) were weak biofilm producers, while
three strains each of B.cepacia, B. cenocepacia and B. pseudomultivorans produced moderate and strong types of biofilms under standard laboratory conditions.
The combination of change in pH with other stress conditions significantly increased the biofilm formation. This study found the difference in the antimicrobial susceptibility pattern of Bcc isolates from ultrasound gels and the adaptability to in-vitro stress conditions explaining the variability of virulence among Bcc species.
Keywords: Burkholderia cepacia Complex (Bcc); Ultrasound Gels; Antimicrobial
Susceptibility Testing; Multiple Antibiotic Resistance Index; Biofilm Formation; Stress Conditions
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