Krithikaa Sekar*

Faculty of Medicine, Sri Lalithambigai Medical College and Hospital, Unit of MGR Educational and Research Institute, Chennai, India

*Corresponding Author: Krithikaa Sekar, Faculty of Medicine, Sri Lalithambigai Medical College and Hospital, Unit of MGR Educational and Research Institute, Chennai, India.

Received: December 12, 2022; Published: January 06, 2023

Abstract

Introduction: Staphylococcus aureus is a major pathogen causing a diversity of life–threatening systemic infections. The MRSA is known to have high probability of PVL toxin gene. The frequency of PVL-producing MRSA in various clinical samples were detected by PCR.

Methods: 218 MRSA isolates from heterogeneous clinical samples received in microbiology laboratory were processed to detect mecA and PVL gene by PCR.

Results: In the present study, out of 218 isolates, 192 were mecA positive by PCR. Of the 192 mecA positive isolates, 104 (54.16%) samples were positive for PVL toxin.

Conclusion: The PCR system used in the study is specific for the mec A and PVL. This may lead to specific therapeutic approaches targeting PVL in severe PVL-related staphylococcal syndromes.

Keywords: MRSA; Mec A; PVL Toxin; PCR

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Citation

Citation: Krithikaa Sekar. “Detection of Panton – Valentine Leukocidin Toxin by Polymerase Chain Reaction in Methicillin Resistant Staphylococcal aureus Isolates". Acta Scientific Microbiology 6.2 (2023): 22-26.

Copyright

Copyright: © 2022 Krithikaa Sekar. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.