Detection of Panton – Valentine Leukocidin Toxin by Polymerase Chain Reaction in Methicillin Resistant Staphylococcal aureus Isolates
Faculty of Medicine, Sri Lalithambigai Medical College and Hospital, Unit of MGR Educational and Research Institute, Chennai, India
*Corresponding Author: Krithikaa Sekar, Faculty of Medicine, Sri Lalithambigai Medical College and Hospital, Unit of MGR Educational and Research Institute, Chennai, India.
December 12, 2022; Published: January 06, 2023
Introduction: Staphylococcus aureus is a major pathogen causing a diversity of life–threatening systemic infections. The MRSA is known to have high probability of PVL toxin gene. The frequency of PVL-producing MRSA in various clinical samples were detected by PCR.
Methods: 218 MRSA isolates from heterogeneous clinical samples received in microbiology laboratory were processed to detect mecA and PVL gene by PCR.
Results: In the present study, out of 218 isolates, 192 were mecA positive by PCR. Of the 192 mecA positive isolates, 104 (54.16%) samples were positive for PVL toxin.
Conclusion: The PCR system used in the study is specific for the mec A and PVL. This may lead to specific therapeutic approaches targeting PVL in severe PVL-related staphylococcal syndromes.
Keywords: MRSA; Mec A; PVL Toxin; PCR
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