Molecular Detection of Helicobacter pylori and its CagA Gene in Upper Gastrointestinal Disease Suspected Patients Living in Shendi Locality, Sudan
Amel Abd Elhafeez S Ali1*, Hadia Abass Eltaib1, Ghanem Mohammed Mahjaf1, Mazin Babekir Musa Bashir1 and Babbiker Mohammed Taher Gorish2
1Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, Shendi University, Sudan
2Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, Omdurman Islamic University, Sudan
*Corresponding Author: Amel Abd Elhafeez S Ali, Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, Shendi University, Sudan.
November 15, 2022; Published: December 29, 2022
Over half of the world's population are chronically infected with Helicobacter pylori (H. pylori), the only bacterium that the WHO has classified as a carcinogen due to its connection to the emergence of gastric cancer. The purpose of this descriptive study was to determine the frequency of H. pylori in a patient suspected with upper gastrointestinal (UGIT) disease using ICT and nested PCR as well as detection of the CagA gene in the Shendi locality. A total of 100 stool samples were collected from patients with UGIT symptoms (60% males and 40% females) with a mean age of 37.2 11.8. Samples were analyzed for the presence of H. pylori antigen by using rapid ICT test , while nested PCR was used to detect H. pylori and its associated CagA gene. Data was collected using a structured questionnaire, and the results were analyzed using (SPSS version 22). Our findings show that the frequency of H. pylori among patients is 89% by ICT and 65% by PCR, respectively. However, the frequency of CagA gene among positive PCR patients was 58.5%. We conclude that there was a high prevalence of H. pylori infection with a high CagA gene producing strain among Sudanese patients in the Shendi locality.
Keywords: H. pylori; CagA Gene; ICT; Nested PCR; Stool Sample; Sudan
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