Rebeca Blancas Landeros^1,2, Gabriela E Olguín-Ruiz², Carlos J Sánchez-Vallejo² and Gloria G Guerrero M³*

¹Escuela Nacional de Ciencias Biológicas, Programa de Licenciatura en Biología, Instituto Politécnico Nacional, CDMX, México

*Corresponding Author: Gloria G Guerrero M, Unidad Académica de Ciencias Biológicas, Universidad Autónoma de Zacatecas, Zacatecas, Zac, Mexico.

Received: November 16, 2022; Published: December 21, 2022

Abstract

Bovine Tuberculosis caused by M. bovis represents an economic and animal health problem worldwide. In Mexico, the biogeography of M. bovis stemmed from studies using genotyping through several genetic markers obtained from SNPs, RFLPs, VNTRs, and Spoligotyping. More recent studies worldwide have used a combination of genotying and whole genome sequencing, in order to cover evolutive history, behavior, phylogeography of the strains. Not whole genome studies in M. bovis from Mexico have been made in detail since in SRA Genbank are deposited only the “reads” of the sequences of the M. bovis isolates. Therefore, in the present study, we focused to carried out the genome assembly of the reads from the SRA database. Phylogenomic analysis show that different isolates of M. bovis strains cluster in the same clade independent of the region, suggesting a close relationship between them. Collectively the data provide an update in the M. bovis phylogeography in Mexico.

Keywords: Bovine tuberculosis; Mycobacterium bovis; Phylogenomics; Comparative Genomics; Mycobacterium Tuberculosis Complex

References

1. World Animal Health Organization (WHO). Global Tuberculosis report. Geneva: World Health Organization. WHO. (2018).
2. World Animal Health Organization (WHO). Enfermedades, infecciones e infestaciones de la Lista de la OIE (2018).
3. Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria (SENASICA). (2021). Informes de la Situación Zoosanitaria Nacional (2021).
4. Palmer MV. “Mycobacterium bovis: Characteristic of Wildlife Reservoir Hosts”. Transboundary and Emerging Diseases 60 (2013): 1-13.
5. O'Connor CM., et al. “Cat-to-Human Transmission of Mycobacterium bovis, United Kingdom”. Emerging Infectious Diseases 25 (2019): 2284-2286.
6. Allen AR., et al. “Does Mycobacterium tuberculosis var. bovis Survival in the Environment Confound Bovine Tuberculosis Control and Eradication? A Literature Review”. Veterinary Medicine International 2021 (2021):
7. Pérez-Gutiérrez I., et al. “Epidemiologia molecular de las tuberculosis bovina y humana en una zona endémica de Querétaro, México”. Salud Pública Méx 50 (2006): 286-291.
8. Milián-Suazo F., et al. “Molecular Epidemiology of human cases of Tuberculosis by Mycobacterium bovis in México”. Preventive Veterinary Medicine 97 (2010): 37-44.
9. Roexie N., et al. “Bovine TB in livestock and wildlife: what’s in the genes?”. Physiology Genomics 45 (2013): 631-637.
10. Garnier TK., et al. “The complete genome sequence of Mycobacterium bovis”.PNAS13 (2010): 7877-7882.
11. Smith NH. “The global distribution and phylogeography of Mycobacterium bovis clonal complexes”. Infection, Genetics and Evolution 12 (2012): 857-865.
12. Je S., et al. “Extent of Mycobacterium bovis transmission among animals of dairy and beef cattle and deer farms in South Korea determined by variable-number tandem repeats typing”. Veterinary Microbiology 176 (2015): 274-281.
13. De Aruda RR., et al. “Genomic and temporal analyses of Mycobacterium bovisin southern Brazil”. Microbial Genomics 7 (2021):

    14.  Kraemer ZC., et al. “Guimaraes AMS. Global distribution and evolution of Mycobacterium bovis lineages”. Frontiers in Microbiology 11 (2020): 843.

15. Gori A., et al. “Spoligotyping and Mycobacterium tuberculosis”. Emerging Infectious Disease 11 (2005): 1242-1249.
16. Supply P., et al. “Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis”. Journal of Clinical Microbiology 44 (2006): 4498-510.
17. Rodwel TCI., et al. “Tracing the origins of Mycobacterium bovis tuberculosis in humans in the USA to cattle in Mexico using Spoligotyping”. International Journal of Infectious Diseases 3 (2016): e129-135.
18. Ghielmetti G., et al. “Epidemiological tracing of bovine tuberculosis in Switzerland, multilocus variable number of tandem repeat analysis of Mycobacterium bovis and Mycobacterium caprae”. PLoS One 12 (2017):
19. Ghebremarian MK., et al. “Genetic profiling of Mycobacterium bovis strains from slaughtered cattle in Eritrea”. PLOS Neglected Tropical Diseases 12 (2018): 1-17.
20. Santillán-Flores MA., et al. “Polymorphism of the PE domains of PE/PE_PGRS sequences in clinical isolates of Mycobacterium bovis in Mexico”. Veterinary Microbiology 115 (2006): 364-369.
21. McLernon J., et al. “Evaluation of mycobacterial interspersed repetitive-unit-variable-number tandem-repeat analysis and Spoligotyping for genotyping of Mycobacterium bovis isolates and a comparison with restriction fragment length polymorphism typing”. Journal of Clinical Microbiology 48 (2010): 4541-4545.
22. Ghavidel M., et al. “The most common Spoligotype of Mycobacterium bovis isolated in the world and the recommended loci for VNTR typing; A systematic review”. Microbe Pathogen 118 (2018): 310-315.
23. Milián-Suazo FL., et al. “Diversidad genética y distribución regional de cepas de Mycobacterium bovis del ganado en México”. Revista Mexicana de Ciencias Pecuarias 3 (2012): 459-471.
24. Milián SF., et al. “Molecular epidemiology analysis of Mycobacterium bovis isolates from Mexico”. American Journal of Veterinary Research 61 (2000): 90-95.
25. Gutiérrez RJA., et al. “Population structure of Mycobacterium bovis isolates from cattle in Mexico”. Preventive Veterinary Medicine 106 (2012): 1-8.
26. Milián-Suazo F., et al. “Molecular relationship between strains of bovis from Mexico and those from countries with free trade of cattle with Mexico”. PloS One 11 (2016): e0155207.
27. Nava VA., et al. “Genetic diversity based on MIRU-VNTR profiles of isolates of Mycobacterium bovis from Mexican cattle”. Preventive Veterinary Medicine 131 (2016): 75-78.
28. Verdugo Escárcega DA., et al. “Analysis of Bovine Tuberculosis Transmission in Jalisco, Mexico through Whole-Genome Sequencing”. Journal of Veterinary Research 64 (2020):
29. Perea-Razo CA., et al. “Whole-Genome Sequencing for detection of Zoonotic Tuberculosis in Querétaro. México”. Journal of Infectious Diseases and Preventive Medicine 5 (2017): 158.
30. Lasserre M., et al. “Whole-genome sequencing of the monophormic pathogen Mycobacterium bovis reveals local differentiation of cattle clinical isolates”. BMC Genomics 19 (2013): 1-14.
31. Fernández-Ramos D., et al. “Molecular typing of Mycobacterium bovis isolates: a review”. Brazilian Journal of Microbiology 45 (2014): 365-372.
32. Zumárraga MJ., et al. “Understanding the relationship between Mycobacterium bovis Spoligoytpes from cattle in Latin American Countries”. Research in Veterinary Science 94 (2013): 9-21.
33. Perea-Razo CA., et al. “Molecular epidemiology of cattle tuberculosis in Mexico through Whole-Genome sequencing and Spoligotyping”. PLoS ONE 13 (2018):
34. Sandoval-Azuara SE., et al. “Whole genome sequencing of Mycobacterium bovis to obtain molecular fingerprints in human and cattle isolates from Baja California, Mexico”. The International Journal of Infectious Diseases 63 (2017): 48-56.
35. Laniado-Laborín R., et al. “Molecular characterization of Mycobacterium bovis isolates from patients with tuberculosis in Baja California, Mexico”. Infection, Genetics and Evolution 27 (2014): 1-5.
36. Nava-Aguilera E., et al. “Clustering of Mycobacterium tuberculosis cases in Acapulco: Spoligotyping and risk factors”. Clinical and Developmental Immunology 2011 (2011): 408375.
37. Gagneux S. “Ecology and evolution of Mycobacterium tuberculosis”. Nature Reviews Microbiology 16 (2018): 202-213.
38. Bertels F., et al. “Automated reconstruction of whole-genome phylogenies from short-sequence reads”. Molecular Biology and Evolution 31 (2014): 1077-1088.
39. Wee WY., et al. “Comparative genome analyses of mycobacteria give better insights into their evolution”. PloS One 12 (2017):
40. Riojas AM., et al. “Phylogenomic analysis of the species of the Mycobacterium tuberculosis complex demonstrates that Mycobacterium africanum, M. bovis, Mycobacterium caprae, Mycobacterium microti and Mycobacterium pinnipedii are later heterotypic synonyms of Mycobacterium tuberculosis”. International Journal of Systematic and Evolutionary Microbiology 68 (2018): 324-332.
41. Loiseau C., et al. “An African origin for Mycobacterium bovis.. Brites D”. Evolution Medicine and Public Health 2020 (2020): 49-59.
42. Rodríguez-Campos S., et al. “Overview and phylogeny of Mycobacterium tuberculosis complex organisms: Implications for diagnostics and legislation of bovine tuberculosis”. Research in Veterinary Science 97 (2014): S5-S19.
43. Smith NH., et al. “Bottlenecks and broomsticks: the molecular evolution of Mycobacterium bovis”. Nature Reviews Microbiology 4 (2006): 670-681.
44. Cerezo-Cortés MJ., et al. “Circulation of  tuberculosisBeijing genotype in Latin America and the Caribbean”. Pathogens and Global Health 113 (2019): 336-351.
45. Juárez-Eusebio DM., et al. “Molecular characterization of multidrug-resistant Mycobacterium tuberculosis isolates from high prevalence Tuberculosis States in Mexico”. Infection, Genetics and Evolution 55 (2017): 384-391.
46. Flores-López CA., et al. “Molecular Epidemiology of Mycobacterium tuberculosis in Baja California, México: A Result of Human Migration?”. Infection, Genetics and Evolution 55 (2017): 378-383.

Citation

Citation: Gloria G Guerrero M., et al. “Phylogenomics analysis of Mycobacterium bovis strains from Mexico: Insight report on its Phylogeography". Acta Scientific Microbiology 6.1 (2023): 42-52.

Copyright

Copyright: © 2022 Gloria G Guerrero M., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.