Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 5 Issue 12

Optimization of Physical Parameters for Bioconversion of Glycyrrhizin into 18α-glycyrrhetinic Acid and 18β-glycyrrhetinic Acid Using Response Surface Methodology

Makhmur Ahmad1, Ambika Chamoli2 and Bibhu Prasad Panda2*

1Department of Pharmaceutics, College of Dentistry and Pharmacy, Buraydah, Al-Qassim, Kingdom of Saudi Arabia

2Microbial and Pharmaceutical Biotechnology Laboratory, Center for Advanced Research in Pharmaceutical Sciences, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India

*Corresponding Author: Bibhu Prasad Panda, Associate Professor (Pharmaceutical Biotechnology), Microbial and Pharmaceutical Biotechnology Laboratory, Center for Advanced Research in Pharmaceutical Sciences, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India.

Received: November 14, 2022; Published: November 22, 2022


Therapeutic constituents in the medicinal plants have always been copious in nature that yield useful phytomolecules. One such plant is licorice which is scientifically known as Glycyrrhiza glabra and belongs to the Fabaceae family. It is interesting to note that Glycyrrhiza has been traditionally utilized in a variety of polyherbal formulations in Japan, known as Kampo medicine. Glycyrrhizin is one of the main constituents of Glycyrrhiza glabra which can be biotransformed into biologically and pharmacologically active 18α-glycyrrhetinic acid and 18β-glycyrrhetinic acid simultaneously. Different levels of physical parameters (temperature, pH and time) was optimized by central composite design (central rotatory) of response surface methodology for the production of hydrolytic unit. Maximum hydrolytic unit of 15.678 HU/ml was predicted at an optimum value of temperature 35ºC, pH 7.8 and time 45 min using response surface plots and point prediction tool of software Design Expert (Stat-Ease Inc., USA).

Keywords: Glycyrrhizin; 18α-glycyrrhetinic Acid; 18β-glycyrrhetinic Acid; Optimization; Central Composite Design; Response Surface Methodology


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Citation: Bibhu Prasad Panda., et al. “Optimization of Physical Parameters for Bioconversion of Glycyrrhizin into 18α-glycyrrhetinic Acid and 18β-glycyrrhetinic Acid Using Response Surface Methodology". Acta Scientific Microbiology 5.12 (2022): 85-92.


Copyright: © 2022 Bibhu Prasad Panda., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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