Pooja Singh Gaur¹, Surya Kant¹*, Ajay Kumar Verma ¹, Anil Kumar Mavi ² and Rachna Chaturvedi³

¹Department of Respiratory Medicine, King George Medical University, Lucknow, Uttar Pradesh, India

*Corresponding Author: Surya Kant, Department of Respiratory Medicine, King George Medical University, Lucknow, Uttar Pradesh, India.

Received: November 08, 2022; Published: November 18, 2022

Abstract

Background: Tuberculosis meningitis (TBM) is the lethal and disabling form of tuberculosis, resulting in increased death and disability rates. The expression of the Leukotriene A4 Hydrolase (LTA4H) gene in TBM helps in the inflammatory pathogenesis of the disease.

Aim: This study aimed to investigate the expression of the LTA4H gene and its association with TBM in the North Indian population.

Methods: This Case-control study was conducted in the Department of Respiratory Medicine in collaboration with the Department of Biochemistry at KGMU, Lucknow, which includes 50 TBM cases and 50 control. LTA4H gene expression was analyzed by using quantitative(q) RT-PCR.

Result: No significant difference in age and gender between case and control group showed adequate (p = 0.61 and p = 0.41). The expression of LTA4H was twofold higher in TBM patients as compared to the control (p < 0.002). White blood cells were elevated in TBM patients as compared to control (19.2 ± 15.7 vs. 8.7 ± 4.2, p <0.0001). LTA4H expression was positively correlated with white blood cells (r = 0.389, p = 0.022) and negatively correlated with aspartate transaminase (r = -0.300, p = 0.038).

Conclusion: The elevated expression of LTA4H mRNA expression is associated with TBM via controlling the inflammation.

Keywords: LTA4H Gene; q-PCR; TBM.

References

1. World Health Organization. “WHO consolidated guidelines on drug-resistant tuberculosis treatment”. World Health Organization (2019).
2. Bahr NC., et al. “Improved diagnostic sensitivity for tuberculous meningitis with Xpert® MTB/RIF of centrifuged CSF”. The International Journal of Tuberculosis and Lung Disease10 (2015): 1209-1215.
3. Patel VB., et al. “Diagnostic accuracy of quantitative PCR (Xpert MTB/RIF) for tuberculous meningitis in a high burden setting: a prospective study”. Plos Medicine10 (2013): e1001536.
4. Nakada T., et al. “The production of colony-stimulating factors by thyroid carcinoma is associated with marked neutrophilia and eosinophilia”. Internal Medicine10 (1996): 815-820.
5. Pormohammad A., et al. “A systematic review and meta-analysis of the diagnostic accuracy of nucleic acid amplification tests for tuberculous meningitis”. Journal of Clinical Microbiology6 (2019): e01113-1118.
6. Trunz BB., et al. “Effect of BCG vaccination on childhood tuberculous meningitis and miliary tuberculosis worldwide: a meta-analysis and assessment of cost-effectiveness”. The Lancet9517 (2006): 1173-1180.
7. Prasad K., et al. “Corticosteroids for managing tuberculous meningitis”. Cochrane Database of Systematic Reviews 4 (2016).
8. Tobin DM., et al. “Host genotype-specific therapies can optimize the inflammatory response to mycobacterial infections”. Cell 3 (2012): 434-446.
9. Nhu NT., et al. “Evaluation of GeneXpert MTB/RIF for diagnosis of tuberculous meningitis”. Journal of Clinical Microbiology1 (2014): 226-233.
10. Thangapandian S., et al. “Molecular dynamics simulation study and hybrid pharmacophore model development in human LTA4H inhibitor design”. PLoS One4 (2012): e34593.
11. Tobin DM., et al. “An enzyme that inactivates the inflammatory mediator leukotriene b4 restricts mycobacterial infection”. PloS One7 (2013): e67828.
12. Ahuja GK., et al. “Diagnostic criteria for Tuberculous meningitis and their validation”. Tubercle and Lung Disease 75 (1994): 149-152.
13. Brose A., et al. “Creatine supplementation enhances isometric strength and body composition improvements following strength exercise training in older adults”. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences1 (2003): B11-19.
14. Ojha P and Sardana V. “Tubercular meningitis presenting as cerebral venous sinus thrombosis: A rare association”. Annals of Indian Academy of Neurology 20 (2017): 1.
15. Patel B., et al. “TB and other chest infections”. Lung India 39 (2022): S43-S85.
16. Morris CW., et al. “The haematological and biochemical changes in severe pulmonary tuberculosis”. Q J Medicine272 (1989): 1151-1159.
17. Nair J., et al. “Association of Leukotriene Gene Variants and Plasma LTB4 Levels with Coronary Artery Disease in Asian Indians”. ISRN Vascular Medicine 2013 (2013): 1-10.
18. Nair J., et al. “Expression analysis of Leukotriene- inflammatory Gene Interaction Network in patients with Coronary Artery Disease”. Journal of Atherosclerosis and Thrombosis 4 (2014): 329-345.
19. Tobin DM., et al. “An enzyme that inactivates the inflammatory mediator leukotriene b4 restricts mycobacterial infection”. PloS one7 (2013): e67828.

Citation

Citation: Surya Kant., et al. “Expression of Leukotriene A4 Hydroxylase Gene and their Association with Tuberculous Meningitis in the North India Population: A Pilot Study". Acta Scientific Microbiology 5.12 (2022): 70-74.

Copyright

Copyright: © 2022 Surya Kant., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.