JM Madu¹*, AI Ogbonna², BO Ojiego¹, F Dantanko¹ and AI Njoku³

¹Department of Environmental Biotechnology Bio Conservation, National Biotechnology Development Agency, Abuja, Nigeria

*Corresponding Author: JM Madu, Department of Environmental Biotechnology Bio Conservation, National Biotechnology Development Agency, Abuja, Nigeria.

Received: November 10, 2022; Published: November 18, 2022

Abstract

The degradation of crude oil by yeasts and bacteria has recently developed become an important beneficial effect in connection with marine pollution. This has helped in the reduction in the dangers of spraying large quantities of toxic chemical detergents and emulsifying agents on crude oil polluted soils. The aim of this study was to evaluate the effect of already isolated indigenous yeast AB-01 on engine oil polluted soil samples for its biodegradation.

The soil samples used were collected from two locations. Sample A was collected from Federal College of Forestry and Sample B was collected from Bauchi Junction, both from Jos metropolis. The samples were collected from 10 cm depth using soil surface sterilized auger. They were taken to the laboratory in clean polythene bags for processing. The polythene bags were labeled accordingly. Each contained about 500g of soil samples. The analysis of different ecological parameters such pH, Moisture content, Organic content and colour and Texture, Rate of water percolation and yeast isolate on formulated medium using 1% engine oil were done.

Results: The results of the ecological parameters of the soil samples revealed that the pH of samples A and B were 5.52 and 5.28 respectively indicating that the soils percentage slightly acidic. The moisture content of the soil samples A and B were found to be 9.75 and 16.80 respectively. The percentage organic content of soil samples A and B w are found to be 22.2% and 11.4% respectively.

The results of water percolation rate of the soil samples A and B revealed that the soils that were bioremedied with the yeast cells drained more water than the control. The result as shown indicates that within the same time limits, soil samples A and B drained more water, 190 ml than the control sample. The results of the inoculation of the yeast isolate on formulated medium using 1% engine oil showed that there was growth of the yeast cells grow on the medium supplemented with engine oil as the sole carbon source. The initiation of growth of the yeast cells grow faster on the medium without engine oil which served as control. The initiation of growth on the engine oil Agar took a longer time of 48 hours before there was growth. After then, the growth flourished.

Keywords: Engine Oil; Degradation; Crude Oil

References

1. Atlas R M. “Stimulated Petroleum Biodegradation”. Critical Reviews in Microbiology 5 (1977): 374-386.
2. Atlas R M and Bartha R. “Hydrocarbon biodegradation and oil spill bioremediation”. Advances in Microbial Ecology 12 (1981): 287.
3. Atlas R M. “Effects of Temperature and Crude Oil Composition on Petroleum Biodegradation”. Applied and Environmental Microbiology 3 (1975): 396-403.
4. Atlas RM and Bartha R. “The Microbiology of Aquatic Spills”. Advances in Applied Microbiology 22 (1977): 225-266.
5. Berntein L. “Osmotic Adjustments of Plants to Saline Media. II Dynamic Phase”. American Journal of Botany 50 (1963): 360-370.
6. Brock TD and Madigan MT. “Biology of Microorganisms”. Publ. Prentice Hall Single Wood Cliffs, New Jersey (1991): 611-676.
7. Christian JHB and Waltho JA. “The Water Relations of Staphylococci and Micrococci”. Journal of Applied Microbiology 25 (1962): 369-377.
8. Curry Lindahl K. “Conservation for Survival An Ecological Strategy”. Publ. Victor, Gollanez Ltd, London, (1974): 27-30.
9. Engas LS and Soldal AV. “Effects of Sersmic Shooting on catch Availability of Cod and Haddock”. publ. Institute of Marine Research (Norway) (1993).
10. Ghaly S and MacDonald N. “Bioremediation; An Innovated Solution for Oil Contamination Final Report (ABC 2002-01), Aventis Biotech. Grade 10”. Sacred Heart, School of Hali Fax (2002).
11. Gordon R. “Oil Pollution and Birds”. Canadian Wildlife Service oil Spill Intelligence Report Cutter Information Corp (1999).
12. Keeler R. “Bioremediation, Healing the Environment Naturally”. R&D Magazines 2 (1996): 34-40.
13. Leung ST., et al. “Pentachlorophenol Biodegradation by Pseudomonas UG25 and UG30”. World Journal of Microbiology 13 (1997): 305-313.
14. “History of Crude Oil Marketing in Nigeria crude Marketing Services (CMS)”. Publ. An Al, Shura International press, Kaduna (1996): 14.
15. Ogunesan MO. “Basic oil Spill Response”. NNPC Occasional publ. Kaduna (1983): 283.
16. Okonkwoeje AI. “The Effects of Different Soil Samples in the Remediation of Crude Oil Polluted Nigerian Agricultural soil”. An M.Sc. Dissertation, University of Jos (2000): 30.
17. RM and Bartha R. “Fate and Effects of Polluting Petroleum in the Marine Environment”. Residue Review 49 (1973): 49-85.

Citation

Citation: JM Madu., et al. “Biodegradation Ability of Yeast Isolate AB – 01 on Engine Oil Polluted Soils in Jos Metropolis". Acta Scientific Microbiology 5.12 (2022): 56-61.

Copyright

Copyright: © 2022 JM Madu., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.