Mendoza-Osorno A^1,2, Olguín-Ruiz GE¹, Sánchez-Vallejo CJ¹, Pérez-Díaz JM³ and Guerrero GG⁴*

¹Instituto Politécnico Nacional, Departamento de Bioquímica, Laboratorio de Genetica Molecular, CDMX, Mexico

*Corresponding Author: Guerrero GG, Universidad Autónoma de Zacatecas, Campus II, Unidad Académica de Ciencias Biológicas, Laboratorio de Inmunobiologia, Zacatecas, Zac, MX, Mexico.

Received: November 02, 2022; Published: November 16, 2022

Abstract

Cadherins are distributed in metazoans. In vertebrates, the most common along with protocadherins are, the classical cadherins, type I and type II. In invertebrates, type III and IV ab cadherins. Here, we focused on the lepidopteran epithelial classical DE-cadherins because they function as a protein-like receptor of Bacillus thuringiensis Cry proteins. Alignment and blasting of protein sequences retrieved from NCBI and a phylogenetic tree was built. A common ancestor and several clades were formed among the different lepidopteran cadherins. The most common are Helicoverpa armigera and Spodoptera litura. Remarkably, lepidopteran cadherins (DE-Cad) from Spodoptera frugiperda (n = 20) matched human E-cadherins with an E value of 1.06 e-47 and a bit score of 177). This data indicate that genes that encode the classical cadherins, present in both, the epithelial midgut of lepidopterans (i.e. Spodoptera frugiperda) and/or the epithelial in mammals, are homologous (around 50%) in the N-terminal region ectodomains, including the conserved free-linkers calcium binding sites. This could have an impact in the diversity of the functionality of these proteins.

Keywords: Classical Cadherins; Vertebrates; Invertebrates; Arthropods; Lepidopteran; Bacillus thuringiensis; Cry Proteins

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Citation

Citation: Guerrero GG., et al. “In silico Analysis of the N-terminal Region of Lepidopteran Cadherins". Acta Scientific Microbiology 5.12 (2022): 43-55a.

Copyright

Copyright: © 2022 Guerrero GG., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.