Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 5 Issue 12

Biochemical Composition and Effects of Aqueous Extracts of the Leaf, Stem and Root of Securidaca longipedunculata Fresen (Violet Tree) on Alloxan Induced Diabetic Rats

Ogbonna AI1*, Madu JM2, Onyimba IA3, Ogbonna USA4, Ejembi EP1, Nwibari BMW5, Tanko JS6, Peter MK7, Ogbonna CIC1, Azi S8 and Damen MT1

1Department of Plant Science and Biotechnology, University of Jos, Nigeria

2National Biotechnology Development Agency, Lugbe, Airport Road, Abuja, Nigeria

3Department of Science Laboratory Technology, University of Jos, Nigeria

4Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Nigeria

5Department of Zoology and Environmental Biology, University of Calabar, Nigeria

6Department of Science Laboratory Technology, Federal College of Forestry, Jos, Nigeria

7Department of Forestry Technology, Federal College of Forestry, Jos, Nigeria

8Department of Pharmacology, University of Jos, Nigeria

*Corresponding Author: Ogbonna AI, Department of Plant Science and Biotechnology, University of Jos, Nigeria.

Received: October 10, 2022; Published: November 07, 2022

Abstract

Studies were carried out on the effects of aqueous leaf, stem bark and root extracts of Securidaca longipedunculata. Fresen (violet tree) on diabetic albino rats. The powdered plant parts were extracted using water as solvent and using maceration method. The qualitative and quantitative analysis of the biochemical components of the plant parts were carried out using standard methods. The acute toxicity study was conducted using 24 albino rats for each plant part. The effects of aqueous leaf, stem bark and root extracts of S. longipedunculata (200 mg/kg) on blood glucose in normoglycemic and alloxan diabetic rats were also investigated using 25 albino wistar rats. Two mls of each of the extracts were administered orally twice daily for 3 weeks after diabetic induction and the blood glucose level was measured on daily basis using On-call-plus glucometer. The hypoglycemic activity was evaluated by comparing the initial blood glucose level with that of the treated and standard. The screening of the aqueous extracts of the plant parts for biochemical composition revealed the presence of some secondary metabolites of pharmacological significance including alkaloids (15-18.40 mg/kg), cardiac glycosides (23.30-26.10 mg/kg), flavonoids (23.58-31.05 mg/kg), saponins (215.60-270.59 mg/kg) and tannins (314.76-339.75 mg/kg) with tannins and saponins in larger quantities. The leaf, stem and root extracts were found to have LD50 value of 490 mg/kg, 693 mg/kg and 693 mg/kg body weight respectively. The plant part extracts showed significant (p ≤ 0.05) reduction of Blood Glucose Concentration (BGC) of 3.47, 3.96 and 4.03 mmol/dl for leaf, stem bark and root extracts respectively after the treatment period indicating that S. longipedunculata has hypoglycemic activity.

Keywords: Securidaca longipedunculata; Biochemical Components; Diabetic; Albino Rats; Extracts

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Citation

Citation: Ogbonna AI., et al. “Biochemical Composition and Effects of Aqueous Extracts of the Leaf, Stem and Root of Securidaca longipedunculata Fresen (Violet Tree) on Alloxan Induced Diabetic Rats". Acta Scientific Microbiology 5.12 (2022): 12-22.

Copyright

Copyright: © 2022 Ogbonna AI., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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