Dr Ramesh Londonkar¹* and Maithilee Kesralikar²

¹Professor, Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka, India

*Corresponding Author: Ramesh Londonkar, Professor, Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka, India.

Received: October 11, 2022; Published: October 31, 2022

Abstract

In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervain (verbenaceae) consists of over 100 genera and nearly 2600 species. Among the Verbenaceae family is Stachytarpheta, a green perennial herb. This species is being examined in this study for its phytochemical, antioxidant, and anti-inflammatory properties. Stachytarpheta mutabilis methanol extracts were studied for their phytochemical screening, antimicrobial, anti-inflammatory, and anticancerous effects in the current study. Phytochemical study revealed the presence of alkaloids, flavonoids, glycosides, steroids, tannins, carbohydrates, and saponins, among other phytoconstituents. One fungus, Aspergillus niger, Staphylococcus aureus, and Bacillus subtilis, as well as four gram (+) pathogens, including Staphylococcus aureus and Bacillus subtilis, and two gram (-) pathogens, including Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae, were performed by disc diffusion tested for antibacterial activity .The nitric oxide scavenging assay was used to test for anti-inflammatory efficacy in vitro. DPPH, ABTS, and total antioxidant tests were conducted. Analyses such as TLC, GC-MS, and were performed as per common procedure. A nitric oxide scavenging experiment was utilised to determine the anti-inflammatory properties of the drug at in vitro. Tests for antioxidant activity was conducted using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) , and total antioxidant tests. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) analysis has been used to do anticancer screening. Extracts from Stachytarpheta mutabilis were found to consist flavonoids, tannins and phenolic compounds as well as terpenoids and steroids in their phytochemical analysis. Squalene, octadecanal, and butan-1-one were found in the GC-MS study indicated other bioactive chemicals such as 4-isopropylcinnamic acid and octadecanoic acid. The methanolic fractions of plant extracts were shown to be effective against all bacteria. Anti-inflammatory and antioxidant experiments were performed in the lab revealed potent inhibition activity of the compound. Analysis of the GC-MS data has revealed the existence of thirty distinct components. MTT assay on the extract indicated anticancer activities of The data shows that the methanolic extract has a significant properties. Stachytarpheta mutabilis has therapeutic value and other pharmaceutical qualities may be assessed as a result of these results, which support the traditional usage of folk medicine as well.

Keywords: Medicinal Plants; Phytochemical Analysis; Antimicrobial; Anti-Inflammatory; Anticancer

References

1. M Idu., et al. “Preliminary Phytochemistry, Antimicrobial Properties and Acute Toxicity of Stachytarpheta jamaicensis (L.) Vahl. Leaves”. Trends in Medical Research 2 (2007): 193-198.

2. P Majorie Liew and Y Keong Yong. “Stachytarpheta jamaicensis (L.) Vahl: From Traditional Usage to Pharmacological”. Evidence Evidence-Based Complementary and Alternative Medicine (2016).
3. CK Kokate., et al. “Textbook of pharmacognosy”. Nirali prakasan. Pune (2002): 1-4.
4. HN Krishna Kumar., et al. “Phytochemica screening and antibacterial activity of stachytarpheta indica”. IJPSR6 (2012): 1684-1687.
5. I A Holder and S T Boyce. “Agar well diffusion assay testing of bacterial susceptibility to various antimicrobials in concentrations non-toxic for human cells in culture”. Burns 20 (1994): 426-429.
6. S Magaldi., et al. “Well diffusion for antifungal susceptibility testing”. International Journal of Infectious Diseases 1 (2004) 39-45.
7. A Chinelo., et al. “Evaluation of various parts of Stachytarpheta angustifolia (Mill.) Vahl for phytochemical, proximate, mineral and vitamin constituents”. Biosciences Research in Today’s World1 (2015): 72-76.
8. Altemimi and Ammar., et al. “Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts”. Plants (Basel, Switzerland) 4 (2017): 42.
9. L Marcocci., et al. “The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761”. Biochemical and Biophysical Research Communications 201-2 (2017): 748-755.

10. VA Gadow., et al. “Comparison of antioxidant activity of aspalathin with that of other plant phenols of Rooibosed tea (Aspalathon linearis), α-tocopherol, BHT and BHA”. Journal of Agricultural and Food Chemistry 45 (20171): 632-638.

11. GC Yen and PD Duh. “Scavenging effect of methanolic extracts of peanut hulls on free-radical and active oxygen species”. Journal of Agricultural and Food Chemistry 42 (1994): 629-632.
12. R Re., et al. “Antioxidant activity applying an improved ABTS radical cation decolorization assay”. Free Radical Biology and Medicine 26 (1999): 9-10.
13. P Prieto., et al. “Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E”. Analytical biochemistry2 (1999): 337-341.
14. ZS Ololade., et al. “Stachytarpheta jamaicensis Leaf Extract: Chemical Composition, Antioxidant, Anti-Arthritic, Anti-Inflammatory and Bactericidal Potentials”. Journal of Scientific and Innovative Research4 (2017): 119-125.
15. SP Mehandra., et al. “Immunopharmacology”. 56 (2018): 156-167.
16. S Princely., et al. “Preliminary phytochemical screening and antimicrobial activity of aerial parts of Stachytarpheta indica (Vahl.)”. Medicinal Plants 5.2 (2013): 96-101.
17. N Vaou., et al. “Towards Advances in Medicinal Plant Antimicrobial Activity: A Review Study on Challenges and Future Perspectives”. Microorganisms10 (2021): 2041.
18. G Faye., et al. “Survey and Antimicrobial Activity study of Ethnomedicinal Plants in Selected Districts of North Shewa Zone, Oromia, Ethiopia”. Infection and Drug Resistance 14 (2021): 5511-5520.
19. C Ogbiko., et al. “Phytochemical, Quantitative Proximate and In Vitro anti-inflammatory study of crude methanolic extract of Stachytarpheta indica leaves (erbenaceae)”. Earthline Journal of Chemical Sciences1 (2019): 153-162.
20. M J Banguo., et al. “Antioxidant and Antibacterial Activities of Five verbenaceae species from Burkina Faso”. Current Research Journal of Biological Sciences 6 (2012): 665-672.
21. S R Sahoo., et al. “Phytochemical screening and bioevaluation of medicinal plant Stachytarpheta indica (L.) Vahl”. Pharmacology and Toxicology Research 2 (2014): 1-5.
22. , et al. “Free radical scavenging and anti-inflammatory properties of Lagerstroemia speciosa (L)”. Inflammopharmacology 16.4 (2008): 182-187.
23. S Sasidharan., et al. “Extraction, isolation and characterization of bioactive compounds from plants extracts”. African Journal of Traditional, Complementary and Alternative Medicines 1 (2011): 1-10.
24. S Yasmeen., et al. Food Science and Biotechnology4 (2017): 1093-1101
25. P R Twentyman., et al. British Journal of Cancer 56 (1987): 279-285.

Citation

Citation: Dr Ramesh Londonkar and Maithilee Kesralikar “Phytochemical and In Vitro Anticancer Activities of Methanolic Extract of Stachytarpheta mutabilis". Acta Scientific Microbiology 5.11 (2022): 85-94.

Copyright

Copyright: © 2022 Dr Ramesh Londonkar and Maithilee Kesralikar. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.