Attapon Cheepsattayakorn^1,3*, Ruangrong Cheepsattayakorn² and Porntep Siriwanarangsun³

¹10^th Zonal Tuberculosis and Chest Disease Center, Chiang Mai, Thailand

*Corresponding Author: Attapon Cheepsattayakorn, 10^th Zonal Tuberculosis and Chest Disease Center, Chiang Mai, Thailand.

Received: October 17, 2022; Published: November 01, 2022

Recently, a study demonstrated an engineered banana lectin (BanLec, Lens culinaris) that importantly decreased mitogenicity by replacing histidine 84 with a threonine (H84T-BanLec) and introduction of a single amino acid substitution [1]. H84T-Banlec demonstrated potent broad-spectrum against viruses high-mannose N-glycans (particularly oligomannose-type glycans and GlcNAC at the non-reducing end terminus and artificial mutants at N-/O-linked glycosylation with most potent and broad antiviral activity against the epidemic variants B.1.1.7, B.1.351, and P.1 [2]), such as severe-acute-respiratory-syndrome coronavirus (SARS-CoV-2 (COVID-19)) and middle-east-respiratory-syndrome coronavirus (MERS-CoV) in vitro and in vivo, in addition to Ebola virus, human immunodeficiency viruses 1 and 2, hepatitis C virus, and influenza viruses A and B, via highly-glycosylated-viral proteins for blocking the cell attachment, cell entry, fusion of membrane, and coronavirus replication [1,3-5]. At 1 mg/mL, lentil lectin revealed no cytotoxic activity and weak hemagglutination [2]. In mouse model, with single injection, no weight loss was identified [2]. Lentil lectin could block inhibit the binding of the human anti-converting-enzyme-2 (ACE 2) receptors to SARS-CoV-2 (COVID-19)-S-trimer protein [2,6]

References

1. Boudreaux DM., et al. “Engineering a therapeutic lectin by uncoupling mitogenicity from antiviral activity”. Cell 163 (2015): 746-758.
2. Wang W., et al. “Lentil lectin derived from Lens culinaris exhibit broad antiviral activities against SARS-CoV-2 variants”. Emerging Microbes and Infection 10 (2021): 1519-1529.
3. Swanson MD., et al. “A lectin isolated from bananas is a potent inhibitor of HIV replication”. Journal of Biological Chemistry 285 (2010): 8646-8655.
4. Coves-Datson EM., et al. “A molecularly engineered antiviral banana lectin inhibits fusion and is efficacious against influenza virus infection in vivo”. Proceedings of the National Academy of Sciences of the United States of America 117 (2020): 2122-2132.
5. Coves-Datson EM., et al. “Inhibition of Ebola virus by a molecularly engineered banana lectin”. PLOS Neglected Tropical Diseases 13 (2019): e0007595.
6. Zhu R., et al. “A molecularly engineered, broad-spectrum anti-coronavirus lectin inhibits SARS-Co-2 and MERS-CoV infection in vivo”. Cell Reports Medicine 3 (2022): 100774.

Citation

Citation: Attapon Cheepsattayakorn., et al. “Lentil Lectin, A Molecularly Engineered from Lens culinaris: A Broad-Spectrum Activities Against SARS-CoV-2 (COVID-19)". Acta Scientific Microbiology 5.12 (2022): 01-02.

Copyright

Copyright: © 2022 Attapon Cheepsattayakorn., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.