Priyanka Dhyani¹, Rashmi²*, Priti Singh³, AD Urhekar⁴ and Samir Pachpute⁵

¹Student - MSc, Medical Microbiology, MGM School of Biomedical Sciences, Maharashtra, India

*Corresponding Author: Rashmi, PhD- Scholar, Department of Microbiology, NIMS University, Rajasthan, India.

Received: September 26, 2022; Published: October 18, 2022

Abstract

Background: Septicaemia is a common cause of morbidity and mortality in neonates and children. The detection of microorganisms in a patient’s blood has a great diagnostic and prognostic significance. Blood culture remains the gold standard for the diagnosis of sepsis.

Objective: 1) To find out the incidence of positive blood culture in suspected cases of septicaemia in children (0-12 years) with antibiotic sensitivity. 2) To study the significance of CRP, and Absolute Neutrophil Count in diagnosis of paediatric bacteraemia/septicaemia and correlation with blood culture results.

Methods: The study was carried out in MGM Medical college and Hospital, Kamothe and MGM hospital, Kalamboli, Navi Mumbai in the period of February 2021 - January 2022. 110 blood samples received in microbiology lab were processed for blood culture and antibiotic sensitivity. CRP test is done by slide agglutination method and Total leucocyte count was done in haematological blood cell counter. Inclusion criteria: Children below 12 years of age and with clinical features suggestive of Bacteraemia/Septicaemia.

Result: Out of 110 samples, 52 (47.27%) samples showed growth and 58 (52.73%) did not show any growth. Among 52 samples showing growth, 46 (79.31%) were the known bacterial pathogens and 6 (10.34%) were skin contaminants (3 CoNS, 3 Candida species). Among the 110 samples 51.82% were negative and 48.18% were positive for CRP. Among the 46 positive blood cultures, the ANC Range value was normal in 30 (65.21%), high in 10 (21.73%) and low in 06 (13.06%) cases, mean ANC value was normal in 15 (32.60%), high in 26 (56.5%) and low in 05 (1.08%) of the samples, and mean neutrophil percentage was normal in 18 (39.13%), high in 25 (54.34%) and low in 03 (6.5%) samples.

Conclusion: Out of 110 blood culture samples studied, 52 (47.27%) samples showing bacterial growth 48.18% were CRP positive and 51.82% were CRP negative. In 7 samples, CRP was positive but blood culture was negative. This could be because of prior antibiotic treatment which has inhibited the bacterial growth in the blood cultures. The positive predictive value (PPV) of ANC range, ANC mean and mean neutrophil percentage was 21.8%, 56.5% and 54.3% respectively. The reliable hematological parameters for septicaemia are Mean of Absolute Neutrophil Count and Mean of Neutrophil percentage. Range of Absolute Neutrophil Count in very wide and was normal in majority cases. Hence it is not reliable criteria.

Keywords: Septicaemia; Bacteriamia; C Reactive Protein; Absolute Neutrophil Count; Antibiotic Sensitivity

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Citation

Citation: Rashmi., et al. “Assessment of Bacteraemia/Septicaemia in Paediatric Patients in a Tertiary Care Hospital". Acta Scientific Microbiology 5.11 (2022): 17-21.

Copyright

Copyright: © 2022 Rashmi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.