Javed Ahamad Khan^1,3, Ram Swaroop Rathore², Iqbal Ahmad³*, Rubina Gill⁴, Fohad Mabood Husain⁵, Javeed Akhtar⁶

¹Department of Natural Resource Management, Bedele Campus of Agriculture and Forestry, Mettu University, Ethiopia

*Corresponding Author: Iqbal Ahmad, Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, India.

Received: August 05, 2022; Published: September 22, 2022

Abstract

Staphylococcus aureus is a Gram positive cocci bacterium causes food poisoning in human population due to consumption of contaminated food. The bacterium may be part of the normal human microbiota which can cause wide range of diseases from skin and soft-tissue infections to severe invasive disease such as infective endocarditis, osteomyelitis, and toxic shock syndrome. In past, the antibiotic resistance of S. aureus has increased manifolds due to the bacterial evolution and antibiotic exploitation of antibiotics. Therefore, screening of antibiotic resistance in S. aureus time to time can be useful enormously for the treating S. aureus associated infections. Hence, this study was intended to investigate the status of antibiotic resistance of S. aureus in beef of buffalo origin. Fifty raw beef samples were randomly collected from different retail butcheries shops in Bareilly city, northern India and determined the load and prevalence of S. aureus. The mean values of S. aureus count were observed from log₁₀ 1.0 cfu/g to log 3.6 cfu/g. Beef samples were also screened for the prevalence (36.0%) of the S. aureus following cultural methods of BAM/CFSAN and isolated eighteen S. aureus isolates. All isolates were confirmed by polymerase chain reaction (PCR) targeting nuclease (nuc) gene. The isolates were scrutinized for antibiotic resistance. The utmost resistance were showed for penicillin (72.2%) and ampicillin (44.4%). The high prevalence (94.4%) of multidrug resistance (MDR) was monitored in S. aureus isolates. High prevalence of buffalo beef with antimicrobial‑resistant S. aureus exhibits an alarming situation and obliges for specific utilization of antibiotics in buffaloes in the study area.

Keywords: S. aureus; Beef; nuc Gene; Multidrug Resistance (MDR)

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Citation

Citation: Iqbal Ahmad., et al. “High Prevalence of Multidrug Resistant Staphylococcus aureus from Buffalo Beef Sold at Retail Butcheries in Northern India". Acta Scientific Microbiology 5.10 (2022): 12-21.

Copyright

Copyright: © 2022 Iqbal Ahmad., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.