Seyi Samson Enitan¹*, John Osaigbovoh Imaralu², Ihuomachi Chioma Osunka¹, Michael Olugbamila Dada¹, Effiong Joseph Effiong¹, Chibuike Ernest Ohanu³, Ifeoluwapo Asekun-Olarinmoye⁴, Eguagie Osareniro Osakue¹, Rufus Olusegun Animashaun⁵ and Oluyemisi Ajike Adekunbi¹

¹Department of Medical Laboratory Science, Babcock University, Ilishan-Remo, Nigeria

*Corresponding Author: Seyi Samson Enitan, Department of Medical Laboratory Science, Babcock University, Ilishan-Remo, Nigeria.

Received: June 27, 2022; Published: August 04, 2022

Abstract

The outcome of pregnancy is often threatened in the presence of sexually transmitted disease like gonorrhea which is common among pregnant women. This study was undertaken to investigate the performance of two diagnostic test methods in the screening of Neiserria gonorrheae infection among pregnant women in Ogun state. In total, hundred (100) pregnant women were recruited for this study. Endo-cervical swabs (ECS) were collected in pairs for the culture and RDT method. For the culture method, the swabs were plated on antibiotic incorporated chocolate agar plates to prevent growth of unwanted organisms. Neisseria gonorrheae LabAcon rapid diagnostic test cassette supplied by Hangzhou Biotest Biotech Co., Ltd, China was used for the rapid screening. Conventional methods were used to identify Neisseria gonorrheaeisolates, and disc diffusion was used to assess susceptibility to standard antibiotics using commercially made antibiotic discs. Out of the 100 participants, 12 (12%) tested positive to N. gonorrheae infection using the culture method and 7 (7%) out of which tested positive to N. gonorrheae infection when screened with the RDT kit. Screening using the culture method was more specific and sensitive than the RDT method. The isolates were most sensitive to Erythromycin, Ofloxacin, Ciprofloxacin, Azithromycin and Gentamycin and less sensitive to Tetracycline, Penicillin and Doxycycline. Lack of awareness of Neisseria gonorrheae, recent sexual intercourse, engagement in unprotected sex, number of sexual partners, and recent change in sexual partner were among the risk variables identified in this study as being connected with the occurrence of N. gonorrheae infection. The outcome of this study shows that gonorrhea is present among pregnant women receiving healthcare in BUTH and also gave credence to the superiority of Culture method (Gold standard) over RDT in the screening of gonorrhea.

Keywords: Gonorrhea; Pregnancy; Culture; Rapid Diagnostic Test Kits; Ogun State; Nigeria

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Citation

Citation: Seyi Samson Enitan., et al. “Performance of Two Diagnostic Methods in the Detection of Gonorrhea Among Pregnant Women in Ogun State, Nigeria". Acta Scientific Microbiology 5.9 (2022): 02-15.

Copyright

Copyright: © 2022 Seyi Samson Enitan., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.