Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 5 Issue 8

Nuclear Amyloid Fibrils Detected in Human SH-SY5Y Cells in Presence of Aβ1-42 and LPS

Anna Lia Asti1*, Nicoletta Marchesi2, Teresa Rampino1, Marilena Gregorini1, Marcella Reguzzoni3, Lorena Vailati4, Alessia Pascale2

1Unit of Nephrology, Dialysis and Transplantation, Fondazione I.R.C.C.S. Policlinico San Matteo, Pavia, Italy

2Department of Drug Sciences, Pharmacology Section, University of Pavia, Pavia, Italy

3Department of Medicine and Surgery, University of Insubria, Varese, Italy

4Department of Molecular Medicine, Fondazione IRCCS, Policlinico San Matteo, Pavia, Italy

*Corresponding Author: Anna Lia Asti, Unit of Nephrology, Dialysis and Transplantation, Fondazione I.R.C.C.S. Policlinico San Matteo, Pavia, Italy.

Received: July 13, 2022; Published: July 21, 2022

Abstract

Amyloid-β peptide (Aβ) represents the main component of amyloid plaques in Alzheimer’s disease (AD); Aβ belongs to the group of antimicrobial peptides (AMPs) small peptides that kill pathogens through their antimicrobial activity and also have affinity for bacterial lipopolysaccharide (LPS). If amyloid is part of the antimicrobial mechanism of Aβ, fibrillar material would also be expected to accumulate as long as the innate immune system, correctly or incorrectly perceives an infection. Repeated reactivations of the chronic latent infection are constantly producing new Aß peptide, this situation lasts for a long time in the decades preceding the manifestation of AD, progressively leading to neurodegeneration and neuroinflammation. Aim of this work was to evaluate the concomitant synergizing action of Aβ1-42 and LPS in human SH-SY5Y cells; AMPs and LPS have an amphipatic structure that is able to form heterogeneous micelles, in this way LPS acts as a fibrillogenesis promoter, Furthermore, depending on peptide concentration, the action of Aβ as AMP can be bacteriostatic or bactericidal.

Keywords: Amyloid-beta (Aβ); Bacterial Lipopolysaccharide (LPS); Antimicrobial Peptide (AMP); Blood Brain Barrier (BBB); Alzheimer’s Disease (AD)

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Citation

Citation: Anna Lia Asti., et al. “Nuclear Amyloid Fibrils Detected in Human SH-SY5Y Cells in Presence of Aβ1-42 and LPS". Acta Scientific Microbiology 5.8 (2022): 90-96.

Copyright

Copyright: © 2022 Anna Lia Asti., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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