Usmangani Tabani, Raj Dangariya, Kinjal Jadav, Neha Jangbari and Archana Rana*

Department of Microbiology, Christ College, Rajkot, India

*Corresponding Author: Archana Rana, Department of Microbiology, Christ College, Rajkot, India.

Received: June 10, 2022; Published: July 12, 2022

Abstract

The present study aims to evaluate the phytochemical and antimicrobial activity of methanol extract of stem of Tridax procumbens against some selected bacteria (E. coli, S. enterica typhimurium, S. flexneri, S. aureus). A cold extraction method was issued to obtain the methanol extract from stems of Tridax procumbens. The antimicrobial activities of this extract were studied on the various test microorganisms using the agar well diffusion technique. phytochemical analysis of this extract was done by using chemical methods. Antimicrobial activity of acetone extract from stem of T. procumbens against E. coli was 5.0 + 0.55 mm, S. enterica typhimurium 7.0 + 0.82 mm, S. flexneri 16 + 1.2 mm, and S. aureus 4 + 0.5 mm respectively. Phytochemical analysis showed the presence of chemicals like alkaloids, flavonoids, steroids, and tannins. Methanol extract of the stem of Tridax procumbens showed antimicrobial activity against all the selected bacteria.

Keywords: Antimicrobial Activity; Phytochemical Analysis; Tridax procumbens; Methanol Extracts

References

1. Kushwaha P., et al. “Phytochemical screening and GC-MS studies of the methanolic extract of Tridax procumbens”. International Journal of Pharmaceutical Sciences and Research 5 (2019): 2492-2496.
2. Bharathi Vadivelu., et al. “Antibacterial activity of Tridax procumbens Linn”. International Journal of Pharmaceutical Sciences and Research 3 (2012): 364-367.
3. Zhang J., et al. “Antioxidant activity of ethanol extracts from Tridax procumbens”. Asian Journal of Chemistry 24 (2012): 58-62.
4. Kumar RSAS., et al. “Anti-oxidant, anti-diabetic, antimicrobial and hemolytic activity of Tridax procumbens”. Journal of Chemical and Pharmaceutical Research 8 (2016): 808-812.
5. Bhati-Kushwaha H and Malik CP. “Assessment of antibacterial and antifungal activities of silver nanoparticles obtained from the callus extracts (stem and leaf) of Tridax procumbens L”. Indian Journal of Biotechnology 13 (2014): 114-120.
6. Saritha K., et al. “Mechanism of antibacterial action of the alcoholic extracts of Hemidesmus indicus (L.) R. Br. ex Schult, Leucas aspera (Wild.), Plumbago zeylanica, and Tridax procumbens (L.) R. Br. ex Schult”. Frontiers in Microbiology 6 (2015): 1-9.
7. P Mozhiyarasi and R Anuradha. “Study on phytochemical analysis and antimicrobial activity of Hyptis suaveolens (L.) poit”. Journal of Chemical and Pharmaceutical Research6 (2016): 438-442.
8. Handa, S., et al. “Extraction Technologies for Medicinal and Aromatic Plants”. United Nations Industrial Development Organization and the International Centre for Science and High Technology (2008): 260.
9. Gadiyar A., et al. “Evaluation of the antimicrobial activity of Ocimum sanctum (tulsi) extract against Streptococcus mutans and Lactobacillus acidophilus - An in vitro study”. International Journal of Health Sciences and Research 7.4 (2017): 224-228.
10. Felhi S., et al. “Solvent extraction effects on phytochemical constituents profiles, antioxidant and antimicrobial activities and functional group analysis of Ecballium elaterium seeds and peels fruits”. Journal of Food Science and Technology 37 (2017): 483-492.
11. Trease GE and Evans WC. “Pharmacognosy”. 11^th, Bailliere Tindall, London (1989): 45-50.
12. Bhalodia N R and Shukla VJ. “Antibacterial and antifungal activities from leaf extracts of Cassia fistula l.: an ethnomedicinal plant”. Journal of Advanced Pharmaceutical Technology and Research 2 (2011): 104-109.
13. Valgas C., et al. “Screening methods to determine antibacterial Extract Journal of Pharmaceutical Research International 32.21 (2020): 96-101. activity of natural products”. Brazilian Journal of Microbiology 38 (2007): 369-380.
14. Lutterodt GD., et al. “Antimicrobial effects of Psidium guajava extracts as one mechanism of its antidiarrhoeal action”. Malaysian Journal of Medical Sciences 2 (1999): 17-20.
15. Lee KI., et al. “Anticancer activity of phytol and eicosatrienoic acid identified from perilla leaves”. Journal of Ethanopharmacology 28 (1999): 1107-1112.

Citation

Citation: Archana Rana., et al. “Phytochemical and Antimicrobial Analysis of Methanol Extract of Stem of Tridax procumbens". Acta Scientific Microbiology 5.8 (2022): 66-69.

Copyright

Copyright: © 2022 Archana Rana., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.