Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 5 Issue 8

Mosquito-borne Disease: A Review of the Possible Synergism Between Arboviral Infection and Plasmodium Infection in West Africa, Nigeria

Babatunde Ibrahim Olowu1-4, Favour Akinfemi Ajibade1-4, Kehinde Samuel Adebayo1,2,4 and Skylar Gay5*

1Faculty of Veterinary Medicine, University of Ibadan, Nigeria

2American Society for Microbiology, Washington DC, USA

3Genetics Society of America, Rockville, MD, USA

4Pathological Society of Great Britain and Ireland, London, United Kingdom

5University of Edinburgh, Scotland, United Kingdom

*Corresponding Author: Skylar Gay, University of Edinburgh, Scotland, United Kingdom.

Received: April 25, 2022; Published: July 07, 2022


Background: Mosquitoes are the most prevalent insect vectors in sub-Saharan Africa. These vectors are the leading cause of acute febrile infections within these regions and also a reservoir for many other microorganisms. This led to misdiagnosis and comorbidity with other diseases like Zika virus, dengue fever, and Japanese encephalitis infections. Due to various limitations of the Nigerian public health system, co-infections are not accurately assessed, and outbreaks of arboviral diseases are poorly reported and recorded.

Aims: We aim to offer an evidence-based approach to questions concerning the high mortality rate reported in cases of Malaria, especially in children. These reviewed techniques used in diagnosing malaria, proving it crude nature as an aid to misdiagnosis of malaria for viral diseases that shares overlapping symptoms. we described the different forms of interactions of arboviruses and Plasmodium in insect vectors, and indicated possible synergies. We analyzed various sero-epidemiological models that could aid efficient diagnosis and proposed the best technique for adoption.

Methods: A review of previous studies on the most prevalent febrile diseases in West Africa, Nigeria was conducted by consulting literatures from PubMed, Africa Journals Online, Google Scholar, and other databases to source studies within this niche in previous years. Relevant keywords such as mosquitoes, Plasmodium interaction, serological diagnosis, clinical signs of mosquitoes were used.

Result: The various publications consulted highlighted the possibilities and cases of malaria co-infection with several zoonotic arboviruses. There is good data to support the fact that arboviral infections have often been misdiagnosed as malaria and, in frequent cases, resulted in death reported as malaria mortality. Studies and findings on efficiently preventing misdiagnosis have been reported and discussed in various clinical trials, as presented in the reviewed articles.

Conclusion: The effective use of polymerase chain reactions (PCR, nested PCR, RT-PCR) as a serological model in malaria diagnosis is strongly recommended to completely exclude cases of arboviral infection in the diagnosis of malaria. In addition, adopting sero-epidemiological models will help forecast outbreaks of arboviral infections so that appropriate preventive measures are taken.

Keywords: Arbovirus; Malaria; Co-infection; RDT; PCV; Plasmodium; Mosquito; Dengue; Africa; Nigeria


  1. Khalifa Refaat M A., et al. “Comparative study of the sensilla on antenna and maxillary palps of five culicine mosquitoes in Sohag Governorate, Egypt”. Journal of the Egyptian Society of Parasitology2 (2013): 481-491.
  2. World Health Organization. “World Malaria Report 2020”.
  3. M N Wogu and F O Nduka. "Evaluating Malaria Prevalence Using Clinical Diagnosis Compared with Microscopy and Rapid Diagnostic Tests in a Tertiary Healthcare Facility in Rivers State, Nigeria". Journal of Tropical Medicine (2018).
  4. Chandramohan Daniel., et al. “Use of clinical algorithms for diagnosing malaria”. Tropical Medicine and International Health: TM and IH1 (2002): 45-52.
  5. McMorrow Meredith L., et al. “Challenges in routine implementation and quality control of rapid diagnostic tests for malaria--Rufiji District, Tanzania”. The American Journal of Tropical Medicine and Hygiene3 (2008): 385-390.
  6. Reyburn Hugh., et al. “Overdiagnosis of malaria in patients with severe febrile illness in Tanzania: a prospective study”. BMJ (Clinical research ed.) 329,7476 (2004): 1212.
  7. Mwangi Tabitha W., et al. “Clinical algorithms for malaria diagnosis lack utility among people of different age groups”. Tropical Medicine and International Health: TM and IH 6 (2005): 530-536.
  8. Ojurongbe Olusola., et al. “Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria”. Malaria Research and Treatment 2013 (2013): 308069.
  9. World Health Organization. “World malaria report 2011”. World Health Organization (‎2011)‎.
  10. Kuno Goro and Gwong-Jen J Chang. “Biological transmission of arboviruses: reexamination of and new insights into components, mechanisms, and unique traits as well as their evolutionary trends”. Clinical Microbiology Reviews 18,4 (2005): 608-637.
  11. Mangat Rupinder. and Ted Louie. “Arbovirus Encephalitides”. StatPearls, StatPearls Publishing, 31 August (2021).
  12. Weaver Scott C and William K Reisen. “Present and future arboviral threats”. Antiviral Research2 (2010): 328-345.
  13. Piantadosi Anne and Sanjat Kanjilal. “Diagnostic Approach for Arboviral Infections in the United States”. Journal of clinical microbiology 12 (2020): e01926-19.
  14. “Arthropod-borne and rodent-borne viral diseases. Report of a WHO Scientific Group”. World Health Organization Technical Report Series 719 (1985): 1-116.
  15. Ayorinde Adenola F., et al. “A survey of malaria and some arboviral infections among suspected febrile patients visiting a health centre in Simawa, Ogun State, Nigeria”. Journal of Infection and Public Health1 (2016): 52-59.
  16. Acharya Pragyan., et al. “Host-Parasite Interactions in Human Malaria: Clinical Implications of Basic Research”. Frontiers in microbiology 8 (2017): 889.
  17. Smith Ryan C and Marcelo Jacobs-Lorena. “Plasmodium-Mosquito Interactions: A Tale of Roadblocks and Detours”. Advances in Insect Physiology 39 (2010): 119-149.
  18. Siciliano Giulia., et al. “Critical Steps of Plasmodium falciparum Ookinete Maturation”. Frontiers in Microbiology 11 (2020): 269.
  19. Angrisano Fiona., et al. “Malaria parasite colonisation of the mosquito midgut--placing the Plasmodium ookinete centre stage”. International Journal for Parasitology 42,6 (2012): 519-527.
  20. Pouniotis D S., et al. “Malaria parasite interactions with the human host”. Journal of Postgraduate Medicine1 (2004): 30-34.
  21. Milner Danny A Jr. “Malaria Pathogenesis”. Cold Spring Harbor Perspectives in Medicine1 (2018): a025569.
  22. Su Xin-Zhuan., et al. “Host-Malaria Parasite Interactions and Impacts on Mutual Evolution”. Frontiers in Cellular and Infection Microbiology 10 (2020): 587933.
  23. (n.d.). Tulane University (2022).
  24. Baer Kerstin., et al. “Kupffer cells are obligatory for Plasmodium yoelii sporozoite infection of the liver”. Cellular Microbiology 9,2 (2007): 397-412.
  25. Faik Imad., et al. “Parasite-host interaction in malaria: genetic clues and copy number variation”. Genome Medicine9 (2009): 82.
  26. Demanga Corine G., et al. “The development of sexual stage malaria gametocytes in a Wave Bioreactor”. Parasites and Vectors1 (2017): 216.
  27. Huang Yan-Jang S., et al. “Arbovirus-Mosquito Vector-Host Interactions and the Impact on Transmission and Disease Pathogenesis of Arboviruses”. Frontiers in Microbiology 10 (2019): 22.
  28. Wu Pa., et al. “Arbovirus lifecycle in mosquito: acquisition, propagation and transmission”. Expert Reviews in Molecular Medicine 21 (2013): e1.
  29. Franz Alexander W E., et al. “Tissue Barriers to Arbovirus Infection in Mosquitoes”. Viruses7 (2015): 3741-3767.
  30. Rückert Claudia and Gregory D Ebel. “How Do Virus-Mosquito Interactions Lead to Viral Emergence?”. Trends in Parasitology4 (2018): 310-321.
  31. Sow Abdourahmane., et al. “Concurrent malaria and arbovirus infections in Kedougou, southeastern Senegal”. Malaria Journal 15 (2016): 47.
  32. "What to know about arboviruses" reviewed by Suzanne Falck. Medial News Today (2017).
  33. Martins Marlos Melo., et al. “Arboviral diseases in pediatrics”. Jornal de Pediatria1 (2020): 2-11.
  34. D'Acremont Valérie., et al. “Time to move from presumptive malaria treatment to laboratory-confirmed diagnosis and treatment in African children with fever”. PLoS Medicine1 (2009): e252.
  35. Mbanefo Afoma and Nirbhay Kumar. “Evaluation of Malaria Diagnostic Methods as a Key for Successful Control and Elimination Programs”. Tropical Medicine and Infectious Disease2 (2020): 102.
  36. Matur BM., et al. "Prevalence of malaria parasite among undergraduate students of University of Abuja”. The Nigeria Journal of Parasitology1,2 (2001): 49-52.
  37. Kolluri N., et al. “Towards lab-on-a-chip diagnostics for malaria elimination". Lab Chip 18 (2018): 75-94.
  38. Pham NM., et al. “Malaria and the ‘last’ parasite: how can technology help?”. Malaria Journal 17 (2018): 260.
  39. Mathison Blaine A and Bobbi S Pritt. “Update on Malaria Diagnostics and Test Utilization”. Journal of Clinical Microbiology7 (2017): 2009-2017.
  40. Maguire Jason D., et al. “Production and validation of durable, high quality standardized malaria microscopy slides for teaching, testing and quality assurance during an era of declining diagnostic proficiency”. Malaria Journal 5 (2006): 92.
  41. Wilson Michael L. “Malaria rapid diagnostic tests”. Clinical Infectious Diseases : An Official Publication of the Infectious Diseases Society of America11 (2012): 1637-1641.
  42. Bell David., et al. “Ensuring quality and access for malaria diagnosis: how can it be achieved?”. Nature Reviews Microbiology9 (2006): 682-695.
  43. McMorrow M L., et al. “Malaria rapid diagnostic tests in elimination settings--can they find the last parasite?”. Clinical Microbiology and Infection : The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases 17,11 (2011): 1624-1631.
  44. Johnston Stephanie P., et al. “PCR as a confirmatory technique for laboratory diagnosis of malaria”. Journal of Clinical Microbiology3 (2006): 1087-1089.
  45. Ramírez Ana L., et al. “Searching for the proverbial needle in a haystack: advances in mosquito-borne arbovirus surveillance”. Parasites and Vectors1 (2018): 320.
  46. Vasoo Shawn and Bobbi S Pritt. “Molecular diagnostics and parasitic disease”. Clinics in Laboratory Medicine3 (2013): 461-503.
  47. Roth Johanna M., et al. “Molecular malaria diagnostics: A systematic review and meta-analysis”. Critical Reviews in Clinical Laboratory Sciences2 (2016): 87-105.


Citation: Babatunde Ibrahim Olowu, Favour Akinfemi Ajibade, Kehinde Samuel Adebayo and Skylar Gay. “Mosquito-borne Disease: A Review of the Possible Synergism Between Arboviral Infection and Plasmodium Infection in West Africa, Nigeria". Acta Scientific Microbiology 5.8 (2022): 14-22 .


Copyright: © 2022 Babatunde Ibrahim Olowu., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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