Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 5 Issue 7

Isolation of Marine Microbugs from Mangrove Ecosystem and Screening of Industrially Important Enzymes

Priyanka Sawant1 and Jignasha Thumar2*

1Department of Microbiology and Biotechnology, Gujarat University, Ahmedabad, Gujarat, India

2Department of Microbiology, Government Science College, Gujarat, India

*Corresponding Author: Jignasha Thumar, Department of Microbiology, Government Science College, Gujarat, India.

Received: May 30, 2022; Published: June 10, 2022

Abstract

Mangrove ecosystem are known for their diverse and rich microbiota. Here human influence is limited, so novelty in bacterial diversity is more common as mangroves ecosystem comes under conservative area and human interference in restricted here. In this present study, mangrove sample like mangrove associated Soil, Coastal water, Roots were collected from Borivali coastal area of manori creek and Jhow Island, Maharashtra under prior permission from CCF, Mangrove Cell, Maharashtra government. Halophiles are in demand in many Biotech based company and their enzymes are equally in demand due their properties. Here in the present experiment, with the help of enriched media 30 samples were processed from which 8 samples were from Jhow Island and 22 samples from borivali coastal-line. All the samples were screened with 10% salt and 20% salt concentration (NaCl). Sample were also screened for 7 pH and 9 pH growth parameters from Borivali and Jhow Island respectively. From above mention samples, total 62 Isolates were obtained, which were screened for Bio-Industrially important enzymes like Amylase, Protease and Cellulase. These enzyme have great demand when they are active in high salt concentration or alkaline pH. These enzymes may emphasize more Scientific-Entrepreneur in India.

Keywords:Mangrove Ecosystem; Halophiles; Bio-industry; Scientific-entrepreneur

References

  1. I Dewiyanti., et al. “Cellulase enzyme activity of the bacteria isolated from mangrove ecosystem in Aceh Besar and Banda Aceh”. IOP Conference Series: Earth and Environmental Science 1 (2022): 012113.
  2. B O De Veras., et al. “Screening of protease, cellulase, amylase and xylanase from the salt-tolerant and thermostable marine Bacillus subtilis strain SR60”. F1000Research 7 (2018): 1704.
  3. P Sawant and J Thumar. “Amalgamation of technologies and scientrepreneur prospective of electrochemically active halophilic microbugs - a review paper”. Vidya-A Journal of Gujarat University2 (2021): 125-156.
  4. JT Thumar and SP Singh. “Secretion of an alkaline protease from a salt- tolerant and alkaliphilic, Streptomyces clavuligerus strain Mit-1”. Brazilian Journal of Microbiology 4 (2007): 766-772.
  5. F William and K Catherine. “Microbial Enzymes and Biotechnology”. Microbial Enzymes Biotechnology BOOK (1990).
  6. R C Kasana., et al. “A rapid and easy method for the detection of microbial cellulases on agar plates using Gram’s iodine”. Current Microbiology5 (2008): 503-507.

Citation

Citation: Priyanka Sawant and Jignasha Thumar. “Isolation of Marine Microbugs from Mangrove Ecosystem and Screening of Industrially Important Enzymes". Acta Scientific Microbiology 5.7 (2022): 35-39.

Copyright

Copyright: © 2022 Priyanka Sawant and Jignasha Thumar. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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