Novel 3-strain Combinatorial Probiotics Alleviates Symptoms of Inflammatory Bowel Disease in Mice
Shinjini Mitra and Ena Ray Banerjee*
Immunology and Regenerative Medicine Research Laboratory, Department of Zoology, University of Calcutta, Kolkata, India
*Corresponding Author: Ena Ray Banerjee, Professor, Immunology and Regenerative Medicine Research Laboratory, Department of Zoology, University of Calcutta, Kolkata, India.
May 06, 2022; Published: June 06, 2022
Inflammatory bowel disease (IBD) is a chronic disease of the gastrointestinal tract, with no permanent and safe cure. We designed this study, using a 3-strain novel combinatorial probiotic formulation (ABT) as therapy for IBD. The anti-inflammatory, pro-regenerative, and antibacterial activities of ABT were determined in vitro, and then validated in mice with IBD.
The anti-inflammatory activity of ABT on DSS-treated RAW cells was determined by MTT and NBT assays, and its antibacterial effect was determined against pathogenic bacteria. ABT was administered orally on days 5, 7, 9, 11 and 13, to mice with DSS-induced IBD. After sacrifice on day 14, disease parameters were measured.
ABT showed significant anti-inflammatory and pro-regenerative effects for over 96 hours, and inhibited 4 pathogenic bacterial strains. In mice, ABT successfully reduced symptoms of IBD, evidenced by restoration in body weight, cellular status, tissue structure, and mediators.
We inferred that the individual strains contributed cumulatively to the biological activities of ABT. The bacteria in the ABT adhered to the intestinal epithelium, and initiated repair mechanisms, by downregulating mediators (NFκB, TNFα, IL-1β, NO, and IAP), and upregulating mucus production. Thus, this translationally-valuable novel combination can be an economical and safe therapy for IBD.
Keywords: Combinatorial Probiotics; GRAS; Anti-inflammatory; Antibacterial; Adherence; Pro-regenerative
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