Daniel S Kim¹, Min Zhang², Nhi Le², Seth Harris² and Kyoungtae Kim²*

¹Emory College of Arts and Science, USA
²Department of Biology, Missouri State University, USA

*Corresponding Author: Kyoungtae Kim, Department of Biology, Missouri State University, USA.

Received: April 04, 2022; Published: April 29, 2022

Abstract

Recent literature demonstrates that platinum-based chemotherapeutic drugs in physiological solvents display higher efficacy in destabilizing cancer cells. As human cancer cells come in over 200 different varieties, it would be beneficial to test the efficacy of these drugs using a wider spectrum of cells. Utilizing the well-tested HeLa cervical cancer cells as a control for the effects of these drugs, we assessed the impact of platinum-based cisplatin, carboplatin, and oxaliplatin on ML-1 thyroid cancer cells. Through the XTT Viability assay, we found that ML-1 cells are more resistant to cisplatin and oxaliplatin with an IC₅₀ value at least four times higher than those for the same drugs in HeLa cells. It has been consistently shown that the oxidative stress caused by these chemicals was more pronounced in HeLa cells than in ML-1 cells, but the only measurable results were found 24 hours after treatment. We also show that a high percentage of HeLa cells displayed apoptosis with even 20 mM of these chemicals, which is directly comparable in effect to the 100 mM of chemicals in ML-1 cells. Upon comparing the expression levels of pro-apoptotic enzymes in HeLa and ML-1 cells, we observed that when treated with 40 mM of these chemicals, the levels of pro-apoptotic enzymes were statistically higher for HeLa cells than for ML-1 cells. Our research will provide new insight into the different capacities of each cell line and the treatment regimen for cancer patients in the future.

Keywords: ML-1; HeLa; Cisplatin; Cytotoxicity; Resistance

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Citation

Citation: Kyoungtae Kim., et al. “Effects of Platinum-based Chemotherapeutic Agents on ML-1 Thyroid Cancer Cells". Acta Scientific Microbiology 5.5 (2022): 128-139.

Copyright

Copyright: © 2022 Kyoungtae Kim.,et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.