Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 5 Issue 5

In Vitro Regeneration of Soybean Genotypes and Induction of Drought Stress In Vitro Condition and Influence on Secondary Metabolites in Soybean Callus Culture

Shashank A Tidke1*, S Kiran1, G Kosturkova2 and GA Ravishankar1

1Department of Biotechnology, Dayanada Sagar College of Engineering, Kumara Swamy Layout, Bengaluru, India
2Plant Biotechnology, In Vitro Development and Genome Regulation, Institute of Plant, Physiology and Genetics, Bulgaria Academy of Sciences, Sofia, Bulgaria

*Corresponding Author: Shashank A Tidke, Department of Biotechnology, Dayanada Sagar College of Engineering, Kumara Swamy Layout, Bengaluru, India.

Received: April 01, 2022; Published: April 29, 2022


Soybean (Glycine max L Leguminosae) is an important grain legume that is not only a valuable oil seed crop but also used as feed for livestock and aquaculture. Soybean genotypes viz., CO-1 and JS 335 were used for the study of drought-induced variations in growth. The present investigation was undertaken to study the effect of two concentrations of PEG on callus induction of soybean genotypes. Seeds of soybean were inoculated on MS medium supplemented with two different concentrations of PEG (2% and 4%) cultures were incubated at 26 ± 2°C under 16h photo periods. The effect of different concentration of PEG on callus induction was investigated. In vitro callus cultures of both genotypes (CO-1and JS 335) showed a reduction in callus growth during PEG treatment as compared with the control. The presence of PEG in the medium elevated dry matter content in all treatments compared with the control. Similarly flavonoid levels and phenolic contents were higher in the PEG treatments in comparison to control. Our results can be used for in vitro screening and manipulations of soybean cultivars for improvement of drought tolerance.

Keywords: Glycine max; Flavonoid; Total Phenolic; Polyethylene Glycol (PEG); Relative Growth Rate; Dry Matter Content; Drought Tolerance


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Citation: Shashank A Tidke., et al.In Vitro Regeneration of Soybean Genotypes and Induction of Drought Stress In Vitro Condition and Influence on Secondary Metabolites in Soybean Callus Culture". Acta Scientific Microbiology 5.5 (2022): 121-127.


Copyright: © 2022 Shashank A Tidke.,et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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