Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 5 Issue 5

Cotrimoxazole Resistance Profile of Bacterial Strains Isolated from Urine Samples in Children Born to HIV Positive Mothers Compared to HIV Non-exposed Children

Blandine Nkenfou Kampa1, Michel Kengne1, Ervé-Martial Kuemkon1, Carine Nguefeu Nkenfou-Tchinda2, Hortense Gonsu Kamga3, Bienvenu Bongue4 and Céline Nguefeu Nkenfou5,6*

1Catholic University of Central Africa, School of Health Sciences, Yaounde, Cameroon

2University of Yaounde I, Faculty of Sciences, Department of Biochemistry, Cameroon

3Yaounde University Teaching Hospital, Cameroon

4Université de Saint-Etienne, Centre Technique d’Appui et de Formation des Centres d’examens de santé

5Chantal Biya International Reference Centre, Yaounde, Cameroon

6University of Yaounde I, Higher Teachers’ Training College, Yaounde, Cameroon

*Corresponding Author: Céline Nguefeu Nkenfou, Chantal Biya International Reference Centre, Yaounde, Cameroon.

Received: March 10, 2022; Published: April 12, 2022

Abstract

Background: WHO has recommended a systematical administration of cotrimoxazole to children born to HIV-positive mothers to limit or reduce the occurrence of opportunistic infections. This systematic use of cotrimoxazole is associated with the evident risk of increased antibiotic resistance. We aimed to determine the prevalence of cotrimoxazole resistance and the resistance profile of strains isolated from urine samples of children born to HIV-positive mothers compared to HIV non-exposed, cotrimoxazole naïve children.

Methods: Children presenting with urinary tract infection were enrolled and grouped as cotrimoxazole treated or naïve. Bacteria isolates from urine samples of these children and were identified using standard microbiology techniques. Antibiotic susceptibility testing was performed using the disc diffusion technique. Data were analyzed using the Statistical Package for Social Science (SPSS) version 20.

Results: A total of 137 children under 15 years of age were enrolled. Of these, 98 were not HIV-exposed and cotrimoxazole naïve (group 1) and the remaining 39 HIV-infected were on cotrimoxazole prophylaxis (group 2). The prevalence of urinary tract infection (UTI) was 31.5%; 29.5% in group 1 compared to 70.5% in group 2. Cotrimoxazole resistance was recorded in 77% of bacterial strains isolated from group 1 and 84% in group 2 with no statistical difference. In both groups, the most common bacteria isolated were Escherichia. coli (E; coli) and Klebsiella. Pneumoniae (K. pneumoniae). These strains were resistant to more than 70% of the antibiotics tested, nevertheless they showed a good sensitivity to ciprofloxacin, imipenem and ceftriazone.

Conclusion: This study shows that despite the use of cotrimoxazole as prophylactic, UTI still occurred in HIV infected children, with a global prevalence of almost four-fifth resistance to cotrimoxazole and almost three-quater multi-resistance to other antibiotics. This implies a requirement of antibiogram in case of UTI before prescription.

Keywords: HIV-exposed Children; Cotrimoxazole; Lower Urinary Tract Infection; Bacterial Resistance

References

  1. Ministry of Public Health, Division of health operational research Report of the Cameroon population-based HIV impact assessment (CAMPHIA) (2018).
  2. Adrian J Salter. “Trimethoprim-Sulfamethoxazole: An Assessment of More than 12 Years of Use”. Reviews of Infectious Diseases2 (1982): 196-236.
  3. Fair RJ and Tor Y. “Antibiotics and bacterial resistance in the 21st century”. Perspectives in Medicinal Chemistry 6 (2014): 25-64.
  4. “High levels of antibiotic resistance found worldwide, new data shows (2018).
  5. Dadgostar P. “Antimicrobial Resistance: Implications and Costs”. Infection and Drug Resistance 12 (2019): 3903-3910.
  6. Supplementary section to the 2013 WHO consolidated guidelines on the use of antiretroviral drugs for treating and preventing HIV infection, Chapter 8 - Prevention, screening and management of common coinfections: “Guidelines on Post-Exposure Prophylaxis for HIV and the Use of Co-Trimoxazole Prophylaxis for HIV-Related Infections Among Adults, Adolescents and Children: Recommendations for a Public Health Approach”. December 2014 supplement to the 2013 consolidated guidelines on the use of antiretroviral drugs for treating and preventing HIV infection. Geneva: World Health Organization; 2014 Dec. 5, THE USE OF CO-TRIMOXAZOLE PROPHYLAXIS FOR HIV-RELATED INFECTIONS AMONG ADULTS, ADOLESCENTS AND CHILDREN (2014).
  7. Leroy V and Mariani-Kurkdjian P. “Épidémiologie et diagnostic des infections urinaires”. Médecine Thérapeutique/Pédiatrie3 (2004): 173-179.
  8. Kenkouo GA. “Mémoire” (2008).
  9. Kouri T., et al. “European Urinalysis Guidelines”. Scandinavian Journal of Clinical and Laboratory Investigation 231 (2000): 1-96.
  10. Grimwade K and Swingler G. “Cotrimoxazole prophylaxis for opportunistic infections in adults with HIV”. Cochrane Database Systematic Review3 (2003): CD003108.
  11. Nunn Andrew J., et al. “Role of co-trimoxazole prophylaxis in reducing mortality in HIV infected adults being treated for tuberculosis: randomised clinical trial”. BMJ (Clinical Research edition.)a257 (2007).
  12. Cameroon Ministry of Public Health. National Guideline on the Prevention and Management of HIV in Cameroon (2018).
  13. Kouemo -Motse DG., et al. “Etiologic Profile and Sensitivity Pattern of Germs Responsible for Urinary Tract Infection Among Under-five Children in Douala, Cameroon: A Hospital-Based Study”. Avicenna JOURNAL of Clinical Microbiology and Infection 2 (2019): 49.
  14. Chang SL and Short life LD. “Pediatric urinary tract infections”. Pediatric Clinics of North America 3 (2006): 379-400.
  15. Korbel L., et al. “The clinical diagnosis and management of urinary tract infections in children and adolescents”. Paediatrics and International Child Health 4 (2017): 273-279.
  16. Lee DS., et al. “Community-Acquired Urinary Tract Infection by Escherichia coli in the Era of Antibiotic Resistance”. BioMed Research International (2018): 7656752.
  17. Dossim S., et al. “Infections urinaires communautaires et acquises au CHU Campus”. Journal de la Recherche Scientifique de l'Université de Lomé 4 (2017): 471-476.
  18. Mariani-Kurkdjian P., et al. “Physiopathologie et virulence des Escherichia coli producteurs de Shiga-toxines”. Réanimation. mai3 (2012): 268-279.
  19. Powis KM., et al. “Cotrimoxazole prophylaxis was associated with enteric commensal bacterial resistance among HIV-exposed infants in a randomized controlled trial, Botswana”. Journal of the International AIDS Society 3 (2017): e25021.
  20. Marwa KJ., et al. “Resistance to Cotrimoxazole and Other Antimicrobials among Isolates from HIV/AIDS and Non-HIV/AIDS Patients at Bugando Medical Centre, Mwanza, Tanzania". AIDS Research and Treatment (2015): 8.
  21. Moroh JLA., et al. “Diversity and antibiotic resistance of uropathogenic bacteria from Abidjan”. African Journal of Urology 1 (2014): 18-24.
  22. Morpeth SC., et al. “Effect of trimethoprim-sulfamethoxazole prophylaxis on antimicrobial resistance of fecal Escherichia coli in HIV-infected patients in Tanzania”. Journal of Acquired Immune Deficiency Syndromes5 (2008): 585-591.
  23. Ibeneme CA., et al. “Urinary tract infection in febrile under five children in Enugu, Southeastern Nigeria”. Nigerian Journal of Clinical Practice 5 (2014): 624-628.
  24. Vazouras K., et al. “Antibiotic treatment and antimicrobial resistance in children with urinary tract infections”. Journal of Global Antimicrobial Resistance 20 (2020): 4-10.
  25. Pouladfar G., et al. “The antibiotic susceptibility patterns of uropathogens among children with urinary tract infection in Shiraz”. Medicine (Baltimore)37 (2017): e7834.
  26. Pouwels KB., et al. “Will co-trimoxazole resistance rates ever go down? Resistance rates remain high despite decades of reduced co-trimoxazole consumption”. Journal of Global Antimicrobial Resistance 11 (2017): 71-74.

Citation

Citation: Céline Nguefeu Nkenfou., et al. “Cotrimoxazole Resistance Profile of Bacterial Strains Isolated from Urine Samples in Children Born to HIV Positive Mothers Compared to HIV Non-exposed Children". Acta Scientific Microbiology 5.5 (2022): 73-79.

Copyright

Copyright: © 2022 Céline Nguefeu Nkenfou., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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