Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 5 Issue 5

Can Routine Laboratory Biomarkers Predict Bloodstream Infections by Gram-negative Bacteria?

Daniela Dambroso-Altafini1,2, Thatiany C Menegucci3, Bruno Buranello Costa2, Danielle Shinohara1, Sheila AB Nishiyama1, Josmar Mazucheli4 and Maria Cristina Bronharo Tognim1*

1Department of Basic Health Sciences, State University of Maringá, Maringá, Paraná, Brazil

2Maringá University Hospital, State University of Maringá, Maringá, Paraná, Brazil

3Department of Medicine, University Paranaense, Umuarama, Paraná, Brazil

4Department of Statistic, State University of Maringá, Maringá, Paraná, Brazil

*Corresponding Author: Maria Cristina Bronharo Tognim, Department of Basic Health Sciences, State University of Maringá, Maringá, Paraná, Brazil.

Received: March 08, 2022; Published: April 11, 2022


Bloodstream infections caused by Gram-negative bacteria (BSI-GN) pose clinical challenges due to their severity and difficulty of treatment. Thus, the present study demonstrated the ability of routine laboratorial biomarkers (RLB) values to detect BSI-GN before the final blood culture (BC) report, including RLB values obtained at the time of BC collection (0h) and also 48 and 24 hours before BC collection from patients who had BSI-GN. We retrospectively analyzed data of 6787 patients who collected BC, admitted in a teaching hospital, in Maringa, Brazil. Correlation between RLB and positive BC was assessed using Student's t test or chi-square test. Values of p ≤ 0.01 or p ≤ 0.05 were considered statistically significant. 320 patients (181 BSI-GN and 139 BSI-GP) over 18 years old was included in the study. We evaluated 49 RLB of which 14 showed statistically significant differences (p ≤ 0.01) at T0h. The intra-abdominal initial infections focus showed OR 2.6 (1.37-4.97); p = 0.003, patients with the urinary tract as the initial infectious focus had an OR 2.0 (1.04-4.4); p = 0.04. We concluded that RLB data, analyzed mainly together with the initial infectious focus data, could predict BSI-GN. These analyses could direct in empirical treatment while the BC result is not available.

Keywords:Blood Culture; Laboratory Biomarkers; Bloodstream Infections; Gram-negative; Gram-positive


  1. Nauclér P., et al. "Impact of time to antibiotic therapy on clinical outcome in patients with bacterial infections in the emergency department: implications for antimicrobial stewardship". Clinical Microbiology Infection 27 (2021): 175-181.
  2. Ratzinger F., et al. "Neither Single nor a Combination of Routine Laboratory Parameters can Discriminate between Gram-positive and Gram-negative Bacteremia”. Scientific Report 5 (2015): 16008.
  3. Tang Y., et al. "Inappropriate initial antimicrobial therapy for hematological malignancies patients with Gram-negative bloodstream infections". Springer Berlin Heidelberg; 48 (2020): 109-116.
  4. Tang W., et al. "Hematological parameters in patients with bloodstream infection: A retrospective observational study". Journal of Infection in Developing Countries 14 (2020): 1264-1273.
  5. Hall KK and Lyman JA. "Updated review of blood culture contamination". Clinical Microbiology Reviews 19 (2006): 788-802.
  6. Levy M., et al. "Dysbiosis and the immune system". Nature Reviews Immunology 17 (2017): 219-232.
  7. Chand N and Sanyal AJ. "Sepsis-induced cholestasis”. Hepatology 45 (2007): 230-241.
  8. Shim BS., et al. "Clinical Value of Whole Blood Procalcitonin Using Point of Care Testing, Quick Sequential Organ Failure Assessment Score, C-Reactive Protein and Lactate in Emergency" Department Patients with Suspected Infection”. Journal of Clinical Medicine 8 (2019): 833.
  9. Lavoignet CE., et al. "White blood cell count and eosinopenia as valuable tools for the diagnosis of bacterial infections in the ED". European Journal of Clinical Microbiology and Infectious Diseases 38 (2019): 1523-1532.
  10. Davido B., et al. "Changes in eosinophil count during bacterial infection: revisiting an old marker to assess the efficacy of antimicrobial therapy". International Journal of Infectious Diseases 61 (2017): 62-66.


Citation: Maria Cristina Bronharo Tognim., et al. “Can Routine Laboratory Biomarkers Predict Bloodstream Infections by Gram-negative Bacteria?". Acta Scientific Microbiology 5.5 (2022): 57-61.


Copyright: © 2022 Maria Cristina Bronharo Tognim., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Acceptance rate30%
Acceptance to publication20-30 days

Indexed In

News and Events

  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 10, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US