Irshad Begum¹, Zahid Hussain Soomro¹ and Afsheen Arif²*

¹Department of Chemistry, University of Karachi, Karachi, Pakistan

*Corresponding Author: Afsheen Arif, Karachi Institute of Biotechnology and Genetic Engineering, University of Karachi, Pakistan.

Received: January 17, 2022; Published: April 11, 2022

Abstract

Aims and Objective: Silver nanoparticles (AgNPs) are the tiny particles of silver from 1 to 100 nm in size. The current study describe the preparation of capped AgNPs based on trisodium citrate (TSC) bottom up approach. AgNPs are majorly applied in medicine for diagnostic applications to therapeutic, apart from its antimicrobial activity.

Research Methods: Turkevich method has been used to prepare silver precursor i.e. silver salt (AgNO₃). The ions of silver are reduced by reducing agent for their non-valent metal atom for this research, Trisodium Citrate (Na₃C₆H₅O₇) was used as the reducing agent in this study. However, capping agent used was Adipic acid (AA). Characteristic of the AgNPs/AA were analyzed by UV-Vis spectrophotometer and FT-IR for the analysis of functional groups.

Results and Conclusion: The AgNPs are colloidal, stable, uniform and polydisperse in nature. Their size is less 30 nm.  These silver nanoparticles were checked against six antibiotics (Erythromycin, Gemifloxacin, Ciprofloxacin, Sparfloxacin, Azithromycin, Ofloxacin) for Gram-positive (Enterobacteriaceae, Escherichia coli, Staphylococcus aureus) and Gram-negative bacteria, (pseudomonas aeruginosa, salmonella typhi, klebsiella pneumoniae) ATCC 8885, ATCC 2881, ATCC 8868, ATCC 9353 by disc susceptibility test. The best resistance was shown against Azithromycin for K. pneumoniae.

Significant Impact: Bacterial resistance is increasing with the adaptation of microbes regularly. Current medical strategies are unable to address related to antibacterial and anti-inflammation diseases. These nano-particles may play some role in this quest.

Keywords: Silver Nanoparticles; Bottom-up Approach; Adipic Acid; Azithromycin; K. pneumoniae

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Citation

Citation: Afsheen Arif., et al. “Bottom-up Approach for the Preparation of Capped Silver Nanoparticles and their Antibacterial Activity". Acta Scientific Microbiology 5.5 (2022): 52-56.

Copyright

Copyright: © 2022 Afsheen Arif., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.