Raggi LA and Navarro C*

FAVET, Universidad de Chile, Chile

*Corresponding Author: Navarro C, FAVET, Universidad de Chile, Chile, canavarr@uchile.cl.

Received: March 04, 2022; Published: April 07, 2022

Abstract

Detection and determination of some viruses and the knowledge of their genome is inextricably linked to a reaction carried out by a thermostable enzyme: Polymerase Chain Reaction (PCR) that was visualized during the 1980s in the United States and its invention has been so important that some separated in time in reference to advances in Medicine/Biology as before and after the PCR. The foregoing, without a doubt, constitutes an immeasurable advance in Human and Animal Medicine. It would be enough to turn on some audiovisual news media or read a written medium to find out how the real epidemic caused by SARS-CoV-2 called COVID19 has developed and the detection of the viral agent or how we have found out about the current omicron variant.

Keywords: Nobel Prize, PCR, viral genomes, COVID19

References

1. Kary B. “Mullis – Facts”. NobelPrize.org. Nobel Prize Outreach AB (2022).
2. Mullis K. “Dancing Naked In The Mind Field” (1998).
3. Mullis K and Faloona F. “Specific synthesis of DNA in vitro via a polymerase- catalyzed chain reaction”. Methods Enzymology (1987).
4. Open Access. Cellular and Molecular Biology (2022).
5. Green M and Sambrook J. “Molecular cloning. A Laboratory Manual 4^th Ed” (2012).
6. “Polymerase Chain Reaction (PCR)-Variations to the System” (2022).
7. Sánchez P., et al. “Diagnosis of Salmonella Enteritidis in tissues and intestinal content of experimentally infected chickens in Chile by Polymerase Chain Reaction”. IJMRAS (2019).
8. The National Center For Biotechnology Information (2021).
9. Clustal Omega. “Multiple Sequence Alignment” (2022).
10. “Basic Local Alignment Search Tool” (2022).
11. Fuentes A., et al. “Detection of ul37 gene from Canine Herpes Virus by Polymerase Chain Reaction”. IJMRGE (2022).
12. Carrasco L., et al. “Detection of the canine herpes virus glycoprotein B gene via Polymerase Chain Reaction”. Open Access Research Journal of Multidisciplinary Studies (2022).
13. Salas V., et al. “Phylogenetic analysis of canine distemper virus detected in Chile”. International Journal of Current Research (2018).
14. Navarro C., et al. “The Nucleocapside Protein Gene As Excellent Target For Detection Of Canine Distemper Virus by Reverse Transcriptase-Polymerase Chain Reaction”. American Journal of Biomedical Science and Research (2019).

Citation

Citation: Raggi LA and Navarro C. “Current Detection of Viral Genomes and their Variants". Acta Scientific Microbiology 5.5 (2022): 47-51.

Copyright

Copyright: © 2022 Raggi LA and Navarro C. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.