Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 5 Issue 4

The Effectiveness of The Disinfection Tools of Ultraviolet Radiation Method in Reducing Number of Airborne Microbial in Several Indoors in Hospitals and Offices in Jakarta

Conny Riana Tjampakasari*, Dimas Seto Prasetyo and Yulia Rosa

Department Microbiology, Faculty of Medicine, Universitas Indonesia, Indonesia

*Corresponding Author: Conny Riana Tjampakasari, Department Microbiology, Faculty of Medicine, Universitas Indonesia, Indonesia.

Received: February 18, 2022; Published: March 22, 2022

Abstract

Nosocomial infection as an infection high morbidity and mortality rates in the world is an infection that is multifactorial in nature. Airborne microbial are the main exogenous source of this infection.

Disinfection technology using ultraviolet is growing rapidly and getting a lot of progress along with the increasing need for the cleaning/sterilization process both in hospitals and offices. The purpose of this study was to prove the effectiveness of the disinfection device using the ultra violet radiation method in reducing the number of airborne microbial on indoors. The number of airborne microbial was counted in the room that was examined using an air sampler, carried our before and after disinfection using disinfection tools. The microbes that grew were counted using a colony counter and the dominant microbes were identified using an automatic Vitek 2® Compact with a specific card based on Gram stain. A total of 36 disinfection tools were examined in several indoors hospitals and offices in Jakarta. The decrease in the number of airborne microbial range from 26.04 - 96.30% with an average reduction of 58.57%. There were 7 species of microbes in the room before being disinfected, while 3 species of microbes were found in the room after being disinfected. The dominant microbes found in the room before and after disinfection were Staphylococcus epidermidis, Bacillus sp dan Aspergillus sp. To increase the disinfecting power of disinfection tools, it is necessary to pay attention to the dose of ultra violet by considering the area of the room, the light intensity used, the distance of the light source to the microbes, the length of time of exposure and the type of microbe itself.

Keywords: Disinfection; UV Radiation; Indoors; Airborne Microbial

References

  1. Departemen Kesehatan RI. “Pedoman infeksi nosokomial di rumah sakit”. Direktorat Jendral Bina Pelayanan Medik. Jakarta (2000).
  2. “Infeksi nosokomial problematika dan pengendaliannya”. Salemba Medika. Jakarta (2008).
  3. Departemen Kesehatan RI. “Pedoman instalasi pusat sterilisasi (central sterile supply department/CSSD) di rumah sakit”. DepKes RI. Jakarta (2009).
  4. Raudah Zubaidah T and Santoso I. “Efektivitas sterilisasi metode panas kering pada alat medis ruang perawatan Rumah Sakit Dr. H. Soemarno Sosroatmodjo Kuala Kapuas”. Jurnal Kesehatan Lingkungan1 (2017): 426-430.
  5. Central Bureau of Statistics. “Kalimantan Selatan in figures 2015”. Statistics of Kalimantan Selatan Province (2015).
  6. Hansen D., et al. “Laminar air flow provides high air quality in the operating field even during real operating conditions, but personal protection seems to be necessary in operations with tissue combustion”. International Journal of Hygiene and Environmental Health 6 (2005): 455-460.
  7. Departemen Kesehatan RI. “Standar pelayanan minimal rumah sakit”. Direktorat Jendral Bina Pelayanan Medik. Jakarta (2002).
  8. Wilson BD., et al. “Comprehensive review of ultraviolet radiation and the current status on sunscreens”. Journal of Clinical and Aesthetic Dermatology 9 (2012): 18-23.
  9. The Oxoid Manual Laboratory. 9th Compiled by E.Y. Bridson, England, Oxoid Limited (2006).
  10. Tjampakasari CR., et al. “Quality of airborne bacteria in operating theaters in several hospitals in Jakarta and its surrounding areas in 2018-2019”. Microbiology Indonesia4 (2020): 1-3.
  11. Operator of Manual MAS 100. MAS-100 Professional Microbiological Air Monitoring System (2006).
  12. Fernandez MO., et al. “Assessing the airborne survival of bacteria in populations of aerosol droplets with a novel technology”. Journal Royal Society 16 (2018): 1-11.
  13. Cappuccino JG., et al. “Microbiology a laboratory lanual”. 10th ed. State University of New York San Fransisco: Pearson Benjamin Cummings (2014): 29-207.
  14. Biomerieux Vitek 2-User Manual. Biomerieux (2017).
  15. Nomura T., et al. “Effectiveness of 222-nm ultraviolet light on disinfecting SARS-CoV-2 surface contamination”. American Journal of Infection Control 49 (2021): 299-301.
  16. Wilson BD., et al. “Comprehensive review of ultraviolet radiation and the current status on sunscreens comprehensive review of ultraviolet radiation and the current status on sunscreens”. Clinical Aesthetic Dermatology9 (2012): 18-23.
  17. RN Golden., et al. “The efficacy of light therapy in the treatment of mood disorders: a review and metaanalysis of the evidence”. American Journal of Psychology4 (2005): 656-662.
  18. Gleeson CM., et al. “Treatment of cutaneous sarcoid with topical gel psoralen and ultraviolet A”. British Journal of Dermatology4 (2011): 892-894.
  19. Naritaa K., et al. “Disinfection and healing effects of 222-nm UVC light on methicillin-resistant Staphylococcus aureus infection in mouse wounds”. Journal of Photochemistry and Photobiology B 178 (2018): 10-18.
  20. Marra AR., et al. “No-Touch disinfection methods to decrease multidrug-resistant organism infections: a systematic review and meta-analysis”. Infection Control and Hospital Epidemiology1 (2018): 20-31.
  21. Buonanno M., et al. “207-nm UV light-a promising tool for safe low-cost reduction of surgical site infections. I: in vitro studies”. PLoS One10 (2013): e76968.
  22. Buonanno M., et al. “207-nm UV light-a promising pool for safe low-cost reduction of surgical site infections. II: in-vivo safety studies”. PLoS One6 (2016): e0138418.
  23. Eliasson B and Kogelschatz U. “UV excimer radiation from dielectric-barrier discharges”. Applied Physics B: Lasers and Optics 46 (1988): 299-303.
  24. Dai T., et al. “Ultraviolet c irradiation: an alternative antimicrobial approach to localized infections?” Expert Review of Anti-infective Therapy 10 (2012): 185-195.
  25. Dai T., et al. “UVC light prophylaxis for cutaneous wound infections in mice”. Antimicrobial Agents and Chemotherapy 56 (2012): 3841-3848.
  26. Dai T., et al. “Ultraviolet C light for Acinetobacter baumannii wound infections in mice: potential use for battlefield wound decontamination?” The Journal of Trauma and Acute Care Surgery 3 (2012): 61-67.

Citation

Citation: Conny Riana Tjampakasari., et al. “The Effectiveness of The Disinfection Tools of Ultraviolet Radiation Method in Reducing Number of Airborne Microbial in Several Indoors in Hospitals and Offices in Jakarta". Acta Scientific Microbiology 5.4 (2022): 125-131.

Copyright

Copyright: © 2022 Conny Riana Tjampakasari., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days

Indexed In






News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 10, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US