CRISPR/Cas System: A Revolutionary Technique Applied Potentially from
Diagnosis to Therapeutics in COVID-19
Roshan Roy1, Ashish Srivastava1,2*, Sonal Srivastava1, Taruna Gupta1, Tripti Singhal1, Prashant Kumar1, Sunny Dhir1 and Narayan Rishi1
1Amity Institute of Virology and Immunology, Amity University Uttar Pradesh, Noida, India
2Department of Entomology and Plant Pathology, University of Arkansas, Fayetteville 72701, USA
*Corresponding Author: Ashish Srivastava, Department of Entomology and Plant Pathology, University of Arkansas, Fayetteville 72701, USA.
November 30, 2021; Published: December 23, 2021
The emergence of Coronavirus disease-2019 caused by SARS-CoV-2 has also bought new challenges to the researchers to develop novel therapeutic and diagnostic methods for the pathogen. The preventive vaccines are approved for the public but their role in the development of herd immunity is not clear due to the emergence of new variants of SARS-CoV-2. Therefore, the prime requirement to cope up with the current situation is to develop highly sensitive diagnostics and effective therapeutics to restrict the spread of SARS-CoV-2 among human population. CRISPR/Cas, a talk of the town, is one of the tools that has been used to develop sensitive, rapid and cost-effective diagnostics that may be made available even in the remote areas of the country. The ability of different Cas proteins viz. Cas9, Cas12, and Cas13 in binding and cleaving of dsDNA, ssDNA, and ssRNA, respectively, have been explored for these purposes. The Cas13 based Prophylactic Antiviral CRISPR in huMAN cells (PAC-MAN) and the Specific High-Sensitivity Enzymatic reporter unlocking (SHERLOCK) and Cas 12 based FALUDA technology have already been developed for diagnosis of COVID-19. This review summarizes the role of CRISPR/Cas based approaches for reliable, rapid, and ultrasensitive diagnostics development, and explore the possible therapeutic alternative for combating COVID-19.
Keywords: Coronavirus; SARS-CoV-2; Cas13; Diagnosis; Therapeutic; PAC-MAN
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