Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 5 Issue 1

Assemblies in Synthetic DNA (Nannochloropsis Species) Crown Cells with Bacillus subtilis

Shoshi Inooka*

Japan Association of Science Specialists, Japan

*Corresponding Author: Shoshi Inooka, Japan Association of Science Specialists, Japan.

Received: November 25, 2021; Published: December 13, 2021


DNA crown cells are artificial cells in which the outer membrane is covered with DNA. DNA crown cells (artificial cells) can be easily synthesized using a sphingosine (Sph)-DNA-adenosine mixture (Synthetic DNA crown cells), and then cultured in egg white (DNA crown cells). Numerous varieties of both kinds of DNA crown cells have been prepared using a variety of different DNA sources.

To study the potential applications of DNA crown cells, the assemblies in synthetic DNA crown cells and B. subtilis that are formed when several kinds of synthetic DNA crown cells were mixed with B. subtilis were examined. In addition, the suppression of B. subtilis growth was observed. Further, target bacteria that were recovered from the assemblies were not B. subtilis (original bacteria), but Brevibacillus, which were produced by synthetic DNA crown cells which promoted the elongation of B. subtilis in the assembly. However, more information on the assembly and formation of DNA crown cells is required to better clarify the characteristics of these cells. The present experiment demonstrated for the first time that an assembly comprising synthetic DNA (Nannochloropsis) crown cells and B. subtilis was formed, that the assembly suppressed bacterial growth, and that bacterial transformation (i.e., from Bacillus to Brevibacillus was observed within the assembly. Moreover, cell-like objects were released from the assembly.


Keywords: Synthetic DNA; Nannochloropsis; Crown Cells; Assembly; Bacillus subtilis; Sphingoshine, Sphingoshine-DNA


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Citation: Shoshi Inooka. “Assemblies in Synthetic DNA (Nannochloropsis Species) Crown Cells with Bacillus subtilis". Acta Scientific Microbiology 5.1 (2022): 03-08.


Copyright: © 2022 Shoshi Inooka. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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