Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 5 Issue 1

Assemblies in Synthetic DNA (Nannochloropsis Species) Crown Cells with Bacillus subtilis

Shoshi Inooka*

Japan Association of Science Specialists, Japan

*Corresponding Author: Shoshi Inooka, Japan Association of Science Specialists, Japan.

Received: November 25, 2021; Published: December 13, 2021

Abstract

DNA crown cells are artificial cells in which the outer membrane is covered with DNA. DNA crown cells (artificial cells) can be easily synthesized using a sphingosine (Sph)-DNA-adenosine mixture (Synthetic DNA crown cells), and then cultured in egg white (DNA crown cells). Numerous varieties of both kinds of DNA crown cells have been prepared using a variety of different DNA sources.

To study the potential applications of DNA crown cells, the assemblies in synthetic DNA crown cells and B. subtilis that are formed when several kinds of synthetic DNA crown cells were mixed with B. subtilis were examined. In addition, the suppression of B. subtilis growth was observed. Further, target bacteria that were recovered from the assemblies were not B. subtilis (original bacteria), but Brevibacillus, which were produced by synthetic DNA crown cells which promoted the elongation of B. subtilis in the assembly. However, more information on the assembly and formation of DNA crown cells is required to better clarify the characteristics of these cells. The present experiment demonstrated for the first time that an assembly comprising synthetic DNA (Nannochloropsis) crown cells and B. subtilis was formed, that the assembly suppressed bacterial growth, and that bacterial transformation (i.e., from Bacillus to Brevibacillus was observed within the assembly. Moreover, cell-like objects were released from the assembly.

 

Keywords: Synthetic DNA; Nannochloropsis; Crown Cells; Assembly; Bacillus subtilis; Sphingoshine, Sphingoshine-DNA

References

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  3. Inooka S. “Aggregation of sphingosine-DNA and cell construction using components from egg white”. Integrative Molecular Medicine6 (2016): 1-5.
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  5. Inooka S. “Systematic Preparation of DNA (Akoya pearl oyster) Crown Cells”. Applied Cell Biology Japan 31 (2018): 21-34.
  6. Inooka S. “Preparation of DNA (Nannochloropsis species) Crown Cells Using eggs, sphingoshine and DNA, and subsequent cell recovery”. Applied Cell Biology Japan 32 (2019): 55-64.
  7. Inooka S. “Preparation of Artificial Human Placental Cells”. Applied Cell Biology 27 (2014): 43-49.
  8. Inooka S. “DNA crown cells using sphingoshine, DNA and eggs-Preparation of DNA (Nannochloropsis) crown cells”. In Y. Inamori, S. Inooka and T. Sakai (Eds), Oyo saibo shigen riyougaku. Taiyo Shobo Corporation (Niigata, Japan) 3 (2020): 91-120.
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  10. Inooka S. “The assembly formation by co-cultures of DNA (Escherichia coli) crown cells with Bacillus subtilis cells”. Journal of Biotechnology and Bioresearch5 (2021).
  11. Inooka S. “Microscopic observation of assemblies formed in mixtures of DNA (human placenta) crown cells with Bacillus subtilis”. An Archive of Organic and In-organic Chemical Sciences2 (2021): 658-664.
  12. Inooka S. “Growth suppression of Bacillus subtilis in Co-cultures with DNA (Escherichia coli) Crown Cells, Including Possible Bacterial Transformation within the Assemblies”. Bioscience and Biotechnology Journal1 (2021): 22-25.
  13. Inooka S. “Systematic DNA (Human Placenta) Crown Cells Elongate Bacillus subtills-Elongation Occurs with the Combination of Sphingosine and DNA”. American Journal of Biomedical Science and Research5 (2021): 419-424.
  14. Inooka S. “Assembly and Bacterial Growth Suppression in the Co-cultures of Synthetic DNA (Akoya Pearl Oyster) Crown Cells with Bacillus subtilis”. Applied Cell Biology Japan 34 (2021): 67-84.
  15. Inooka S. “Synthetic DNA Crown Cells Kill Yeast: Relationship with Assembly Formation of Yeast”. Civil Engineering Research Journal 2 (2021): CERJ.MS.ID555831.

Citation

Citation: Shoshi Inooka. “Assemblies in Synthetic DNA (Nannochloropsis Species) Crown Cells with Bacillus subtilis". Acta Scientific Microbiology 5.1 (2022): 03-08.

Copyright

Copyright: © 2022 Shoshi Inooka. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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