Acta Scientific Microbiology (ISSN: 2581-3226)

Research ArticleVolume 4 Issue 10

Comparison of ZN Microscopy, Culture on LJ Media and Gene Xpert MTB/RIF Assay in Diagnosis of Extra-Pulmonary Tuberculosis

AS Boinwad1 and JA Iravane2*

1Assistant Professor at Swami Ramanand Teerth Rural Government Medical College, Ambajogai, Maharashtra, India
2M.D. Microbiology, Professor and Head of Department of Microbiology, Government Medical College, Aurangabad, India

*Corresponding Author: Dr. JA Iravane, Professor and Head of Department, Department of Microbiology, Government Medical College, Aurangabad, Maharashtra, India.

Received: August 26, 2021; Published: September 20, 2021

Citation: AS Boinwad and JA Iravane. “Comparison of ZN Microscopy, Culture on LJ Media and Gene Xpert MTB/RIF Assay in Diagnosis of Extra-Pulmonary Tuberculosis". Acta Scientific Microbiology 4.10 (2021): 68-74.


  Tuberculosis is caused by Mycobacterium tuberculosis and classified as pulmonary tuberculosis (PTB) and Extra Pulmonary tuberculosis (EPTB). EPTB account for 15-20 % cases of tuberculosis. EPTB diagnosis is challenging due to inadequate sample volume, pauci-bacillary nature and unusual clinical presentation. This study determined the role of Gene Xpert MTB/RIF assay in diagnosis of EPTB and compared results with conventional smear microscopy and culture on LJ media.

  All the samples coming to our laboratory during January 2015 to June 2016 were processed with Gene Xpert MTB/RIF assay, Smear microscopy and LJ media culture. Total samples examined were 514. 90(17.51%) samples were positive by Gene Xpert MTB/RIF assay of these 15 samples shows Rifampicin resistance. Samples which were positive by culture and smear microscopy were 58(11.28%) and 28(5.45%) respectively.

To conclude, we can say that Gene Xpert MTB/RIF assay is the novel test for rapid diagnosis of Extra Pulmonary Tuberculosis.

Keywords: Gene Xpert MTB/RIF Assay; Extra Pulmonary Tuberculosis


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Copyright: © 2021 AS Boinwad and JA Iravane. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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