Sequence-based Bacteriological Probing of Domestic Water Sources in Kiagbodo, Delta State, Nigeria
T Sampson1* and KBD Yom2
1Department of Microbiology, Rivers State University, Nkpolu-Oroworukwo, Port Harcourt, Nigeria
2Department of Biological Science, Edwin Clark University, Kiagbodo, Delta State, Nigeria
*Corresponding Author: T Sampson, Department of Microbiology, Rivers State University, Nkpolu-Oroworukwo, Port Harcourt, Nigeria.
Received: April 06, 2021; Published: May 07, 2021
Major sources of drinking water originates from underground water which is then made accessible through boreholes and mono pumps. However, the portability of drinking water across the globe has increasingly continued to be in doubt, due to the problem of microbial contamination, prompting the need for water quality assessments in areas where boreholes and mono pumps are major sources of drinking water. Thus, this study was carried out to assess the bacteriological quality of domestic water sources in Kiagbodo, Delta State, Nigeria. Water samples used for domestic purposes were obtained from three different boreholes (labelled BH1, BH2, and BH3) and three different mono pumps (tagged MP1, MP2, and MP3) and then assessed for their total heterotrophic bacterial count (THBC) as well sequencing the bacterial isolates, using the sanger method, for their genomic identities. Results from the study showed that mono pumps had the greater counts of heterotrophic bacteria with a mean Log10 THBC of 4.55 as against, 3.25 mean Log10 THBC counts observed across borehole samples. There was a statistical difference in the bacterial counts between the mono pumps and borehole samples (p < 0.05). The total heterotrophic bacteria counts (THBC) observed across all six water samples (boreholes and mono pumps) ranged from 5.0 × 102 to 2.83 × 105 cfu/ml, which were all well above the permissible THBC standard limit recommended by WHO and FAO (1.2 × 102 cfu/ml) and the CDC (< 5 × 102 cfu/ml). Molecular identification of the bacterial species isolated from the domestic water sources (boreholes and monopumps) revealed the presence of four different genus of bacteria to include Bacillus, Proteus, Staphylococcus and Chryseobacteria. The identified species were Bacillus thuringiensis, Proteus vulgaris, Staphylococcus aureus and Chryseobacterium cucumeris. The sources of domestic water in this community were therefore, observed to be well contaminated, containing large amount of heterotrophic bacteria, thus threatens the health of individuals exposed to these water sources. Treatment of these water sources is therefore recommended to check possible outbreak of waterborne diseases in this locality.
Keywords: Bacteriological Probing; Water Quality; Domestic; Sequencing; Kiagbodo
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