Comparison of MGIT960 with Lowenstein-Jensen Method for Mycobacteria Detection in Pulmonary Tuberculosis and Drug Susceptibility Testing
Kalpana Thangavelu, Imola Jamir, Ellappan Kalaiarasan, Pallam Gopichand, Krishnakumariamma Krishnapriya and Noyal Mariya Joseph*
Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry, India
*Corresponding Author: Noyal Mariya Joseph, Associate Professor, Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research JIPMER, Pondicherry, India.
Received: April 05, 2021; Published: May 07, 2021
Introduction: This study aimed to evaluate the performance of Mycobacterium Growth Indicator Tube 960 (MGIT960) and Lowenstein-Jensen (LJ) medium for recovery of mycobacteria in pulmonary tuberculosis (PTB) and the drug susceptibility testing (DST).
Materials and Methods: A total of 2411 pulmonary specimens were collected from TB suspected patients during the study period (July 2018 to March 2020). Specimens were inoculated into both MGIT960 and LJ medium followed by Ziehl-Neelsen (ZN) and Auramine Phenol (AP) staining. DST was performed in MGIT 960 and LJ medium.
Results: Of 2411 pulmonary specimens, 190 (7.0%) and 153 (5.7%) were culture positive by MGIT960 and LJ, respectively. Of the 190 MGIT positive cultures, 169 (88.9%) were Mycobacterium tuberculosis complex (MTB) and 21 (11.1%) were non-tuberculous mycobacteria (NTM). From 153 LJ positive cultures, 138 (90.2%) and 15 (9.8%) were identified as MTB and NTM, respectively. Contamination rate associated with MGIT960 and LJ culture was 125 (5.2%) and 113 (4.7%), respectively. The average time to detection for MTB in MGIT960 and LJ were 18.17 ± 8.69 and 32.46 ± 10.46 days, respectively (p value < 0.0001) and for NTM was 18.76 ± 10.67 and 21.00 ± 12.12 days, respectively (p value 0.5611). Sensitivity of streptomycin (S), isoniazid (I), rifampicin (R) and ethambutol (E) were 87.5%, 100%, 100% and 90.9%, respectively. Specificity of S, I, R, E were 99.3%, 100%, 100% and 98.6%, respectively.
Conclusion: MGIT960 has better recovery, with shorter time to detection and similar contamination rate in pulmonary specimens compared to LJ method. The DST performance of MGIT960 was comparable to LJ.
Keywords: Tuberculosis; MGIT960; LJ Medium; Contamination Rate; Drug Susceptibility Testing
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