Acta Scientific Microbiology (ISSN: 2581-3226)

Research ArticleVolume 4 Issue 6

Comparison of MGIT960 with Lowenstein-Jensen Method for Mycobacteria Detection in Pulmonary Tuberculosis and Drug Susceptibility Testing

Kalpana Thangavelu, Imola Jamir, Ellappan Kalaiarasan, Pallam Gopichand, Krishnakumariamma Krishnapriya and Noyal Mariya Joseph*

Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry, India

*Corresponding Author: Noyal Mariya Joseph, Associate Professor, Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research JIPMER, Pondicherry, India.

Received: April 05, 2021; Published: May 07, 2021

Citation: Noyal Mariya Joseph., et al. “Comparison of MGIT960 with Lowenstein-Jensen Method for Mycobacteria Detection in Pulmonary Tuberculosis and Drug Susceptibility Testing”. Acta Scientific Microbiology 4.6 (2021): 12-17.

Introduction: This study aimed to evaluate the performance of Mycobacterium Growth Indicator Tube 960 (MGIT960) and Lowenstein-Jensen (LJ) medium for recovery of mycobacteria in pulmonary tuberculosis (PTB) and the drug susceptibility testing (DST).

Materials and Methods: A total of 2411 pulmonary specimens were collected from TB suspected patients during the study period (July 2018 to March 2020). Specimens were inoculated into both MGIT960 and LJ medium followed by Ziehl-Neelsen (ZN) and Auramine Phenol (AP) staining. DST was performed in MGIT 960 and LJ medium.

Results: Of 2411 pulmonary specimens, 190 (7.0%) and 153 (5.7%) were culture positive by MGIT960 and LJ, respectively. Of the 190 MGIT positive cultures, 169 (88.9%) were Mycobacterium tuberculosis complex (MTB) and 21 (11.1%) were non-tuberculous mycobacteria (NTM). From 153 LJ positive cultures, 138 (90.2%) and 15 (9.8%) were identified as MTB and NTM, respectively. Contamination rate associated with MGIT960 and LJ culture was 125 (5.2%) and 113 (4.7%), respectively. The average time to detection for MTB in MGIT960 and LJ were 18.17 ± 8.69 and 32.46 ± 10.46 days, respectively (p value < 0.0001) and for NTM was 18.76 ± 10.67 and 21.00 ± 12.12 days, respectively (p value 0.5611). Sensitivity of streptomycin (S), isoniazid (I), rifampicin (R) and ethambutol (E) were 87.5%, 100%, 100% and 90.9%, respectively. Specificity of S, I, R, E were 99.3%, 100%, 100% and 98.6%, respectively.

Conclusion: MGIT960 has better recovery, with shorter time to detection and similar contamination rate in pulmonary specimens compared to LJ method. The DST performance of MGIT960 was comparable to LJ.

Keywords: Tuberculosis; MGIT960; LJ Medium; Contamination Rate; Drug Susceptibility Testing


  1. Gopalaswamy R., et al. “Of tuberculosis and non-tuberculous mycobacterial infections-a comparative analysis of epidemiology, diagnosis and treatment”. Journal of Biomedical Science 27 (2020): 1-7.
  2. National Tuberculosis Elimination Programme. India TB Report. New Delhi: Central TB Division (2020).
  3. Ratnatunga CN., et al. “The Rise of Non-Tuberculosis Mycobacterial Lung Disease”. Frontiers in Immunology 11 (2020): 303.
  4. Sharma SK., et al. “A prospective study of non-tuberculous mycobacterial disease among tuberculosis suspects at a tertiary care centre in north India”. Indian Journal of Medical Research 150 (2019): 458.
  5. Sharma S and Dhar R. “Nontuberculous mycobacterial diseases: Current diagnosis and treatment”. Astrocyte 4 (2017): 67-74.
  6. Lu D., et al. “Comparison of the Automated Mycobacteria Growth Indicator Tube System (BACTEC 960/MGIT) with Lowenstein-Jensen medium for recovery of mycobacteria from clinical specimens”. American Journal of Clinical Pathology 118 (2002): 542-545.
  7. Negi SS., et al. “Comparison of the conventional diagnostic modalities, bactec culture and polymerase chain reaction test for diagnosis of tuberculosis”. Indian Journal of Medical Microbiology 23 (2005): 29-33.
  8. Zhao P., et al. “Evaluation of a manual identification system for detection of Mycobacterium tuberculosis in a primary tuberculosis laboratory in China”. Journal of International Medical Research 47 (2019): 2666-2673.
  9. Subramanyam B., et al. “Reprocessing of Contaminated MGIT 960 Cultures to Improve Availability of Valid Results for Mycobacteria”. International Journal of Microbiology 3 (2020): 1-3.
  10. Abdel-Aziz N., et al. “A comparative evaluation of the BACTEC MGIT 960 (Mycobacterium Growth Indicator Tube) system with LJ solid medium for diagnosis of pulmonary tuberculosis”. Egyptian Journal of Microbiology 18 (2009): 91-96.
  11. Kotwal A., et al. “Mycobacterium tuberculosis and nontuberculosis mycobacteria co-infection: Two cases from the sub-Himalayan region of North India in a year”. Lung India 34 (2017): 494-496.
  12. Sarro YD., et al. “Simultaneous diagnosis of tuberculous and non-tuberculous mycobacterial diseases: Time for a better patient management”. Clinical Microbiology and Infection 3 (2018): 10.
  13. Diriba G., et al. “Performance of Mycobacterium Growth Indicator Tube BACTEC 960 with Lowenstein-Jensen method for diagnosis of Mycobacterium tuberculosis at Ethiopian National Tuberculosis Reference Laboratory, Addis Ababa, Ethiopia”. BMC Research Notes 10 (2017): 181.
  14. Mishra V., et al. “Evaluation of MGIT over other phenotypic methods for the detection of pulmonary and extrapulmonary TB at a tertiary care centre in North India”. International Journal of Pharmaceutical Sciences and Research 7 (2016): 2568.
  15. Rishi S., et al. “A comparative study for the detection of Mycobacteria by BACTEC MGIT 960, Lowenstein Jensen media and direct AFB smear examination”. Indian Journal of Medical Microbiology 25 (2007): 383.
  16. Satti L., et al. “Evaluation of BACTEC MGIT 960 system for recovery of Mycobacterium tuberculosis complex in Pakistan”. Malaysian Journal of Microbiology 6 (2010): 203-208.
  17. Mahomed S., et al. “Failure of BACTEC MGIT 960 to detect Mycobacterium tuberculosis complex within a 42-day incubation period”. African Journal of Laboratory Medicine 6 (2017): 1-3.
  18. Cruciani M., et al. “Meta- Analysis of BACTEC MGIT 960 and BACTEC 460 TB, with or without Solid Media, for Detection of Mycobacteria. J Clin Microbiol 42 (2004): 2321-2325.
  19. Tortoli E., et al. “Use of BACTEC MGIT 960 for Recovery of Mycobacteria from Clinical Specimens: Multicenter Study”. Journal of Clinical Microbiology 37 (1999): 3578-3582.
  20. Smaoui S., et al. “Evaluation of the BACTEC MGIT 960 TB with solid media for recovery of mycobacteria from extrapulmonary specimens in South Tunisia”. Journal of Medical Diagnostic Methods 4 (2015): 1-5.
  21. Saini D., et al. “Comparison of BACTEC MGIT with conventional methods for detection of mycobacteria in clinically suspected patients of extra pulmonary tuberculosis in a tertiary care hospital”. International Journal of Research in Medical Sciences 5 (2017): 3530-3533.
  22. Idigoras P., et al. “Comparison of the automated nonradiometric Bactec MGIT 960 system with Lowenstein-Jensen, Coletsos, and Middlebrook 7H11 solid media for recovery of mycobacteria”. European Journal of Clinical Microbiology and Infectious Diseases 19 (2000): 350-354.
  23. Hasan M., et al. “Evaluation of the effectiveness of BACTEC MGIT 960 for the detection of mycobacteria in Bangladesh”. International Journal of Mycobacteriology 2 (2013): 214-219.
  24. Zhao P., et al. “Evaluation of BACTEC MGIT 960 System for Testing Susceptibility of Mycobacterium tuberculosis to First-Line Drugs in China”. PLoS One 9 (2014): e99659.

Copyright: © 2021 Noyal Mariya Joseph., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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