Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 4 Issue 3

Optimizing the Fermentation Conditions and Enhancing the Keratinase Production from Streptomyces coelicoflavus

Jadhav RS1, JK Oberoi2, Tejashree Rokade2, Rajesh Shingade2, Mrunal Yadav2, Tooba Momin2*

1Department of Microbiology, Vishwasrao Niak Arts, Commerce and Baba Naik Sciences, Mahavidyalaya, Shirala, India
2Department of Microbiology, Abeda Inamdar Senior College, Pune, India

*Corresponding Author: Tooba Momin, Department of Microbiology, Abeda Inamdar Senior College, Pune, India.

Received: October 12, 2020; Published: February 10, 2021

×

Abstract

  Total eight soil samples were taken from Sangli district (Maharashtra) India. The potent isolates was identified by using morphological, cultural, biochemical, physiological and 16S rRNA gene sequence analysis. Keratinase production was optimized using different parameters. The 120 hrs incubation period of was observed for optimum keratinolytic protease production. Inoculum age of 10 days was found to be effective in terms of protease production and degradation. 3% inoculum size was showed optimum degradation and proteolytic activity. The agitation rate at 160 rpm was found to be optimum. Optimum enzyme production and feather degradation was found at pH 9.0. Maximum production of protease was observed at 400C. The optimum amount of keratinase was produced by Streptomyces coelicoflavus in presence of dextrose and peptone as a carbon and nitrogen source separately respectively. 1% feather concentration was found optimum keratinase production. Streptomyces coelicoflavus was enhanced feather degradation in the presence of KH2PO4.

Keywords: Streptomyces coelicoflavus, 16S rRNA, Keratinase, Optimization and Degradation.

×

References

  1. Adelere IA and Lateef A. “Degradation of keratin biomass by different microorganisms”. In: Sharma S, Kumar A, eds. Keratin as a Protein Biopolymer. Cham: Springer (2019): 123-162.
  2. Lateef A., et al. “Keratinolytic activities of a new feather degrading isolate of Bacillus cereus LAU 08 isolated from Nigerian soil”. International Biodeterioration and Biodegradation 64 (2010): 162-165.
  3. Gortner R A and Hoffman W F. “L-cystine”. Organic Syntheses 1 (1941): 194-198.
  4. Papadopoulos M C. “The effect of enzymatic treatment on amino acid content and nitrogen characteristics of feather meals”. Animal Feed Science and Technology 16 (1986): 151-156.
  5. Sun F., et al. “Purification and biochemical characteristics of the extracellular protease from Pediococcus pentosaceus isolated from Harbin dry sausages”. Meat Science (2019): 156-165.
  6. Schmid R D and Verger R. “Lipases: interfacial enzymes with attractive applications”. Angewandte Chemie 37 (1998): 1608-1633.
  7. Jadhav R S., et al. “Isolation, and Characterization of Keratinolytic Streptomyces coelicoflavus”. World Journal of Pharmaceutical Research6 (2015): 1829-1838.
  8. Ramnani P and Gupta R. “Optimization of medium composition for keratinase production on feather by Bacillus licheniformis RG1 using statistical methods involving response surface methodology”. Biotechnology and Applied Biochemistry11 (2004): 191-196.
  9. Anbu P., et al. “Purification of keratinase from poultry farm isolate—Scopulariopsis brevicaulis and statistical optimization of enzyme activity”. Enzyme and Microbial Technology 36 (2005): 639-647.
  10. Kumar R., et al. “Optimization of influential parameters for extracellular keratinase production by Bacillus subtilis (MTCC 9102) in solid state fermentation using horn meal - a biowaste management”. Applied Microbiology and Biotechnology 160 (2010): 30-39.
  11. Shirling EB and Gottlieb D. “Methods for characterization of Streptomyces species”. International Journal of Systematic Bacteriology 16 (1966): 312-340.
  12. Jadhav R., et al. “Isolation, Identification and Characterization of Keratinolytic Streptomyces coelicoflavus”. International Journal of Current Microbiology and Applied Sciences 7 (2016) 153-163.
  13. Riffel A and Brandelli A. “Keratinolytic bacteria isolated from feather waste”. Brazilian Journal of Microbiology 37 (2006): 395-399.
  14. Jain Richa., et al. “Feather degradation by Streptomyces exfoliatus CFS 1068”. Annals of Microbiology (2011).
  15. Rayudu K., et al. “Keratinolytic protease production from keratinaceous wastes’. Journal of Recent Advances in Applied Sciences (JRAAS) 28 (2013): 69-72.
  16. Naif Abdullah Al-Dhabi., et al. “Characterization and fermentation optimization of novel thermo stable alkaline protease from Streptomyces sp. Al-Dhabi-82 from the Saudi Arabian environment for eco-friendly and industrial applications”. Journal of King Saud University - Science 32 (2020): 1258-1264.
  17. Yang SS and Wang JY. “Protease and amylase production of Streptomyces rimosus in submerged and solid state cultivation”. Botanical Bulletin- Academia Sinica Taipei 40 (1999): 259-265.
  18. Petinate DG., et al. “Influence of growth medium in protease and pigment production by Streptomyces cyanens”. Memórias do Instituto Oswaldo Cruz 94 (1999): 173-177.
  19. Saha Subhasish., et al. “Nocardiopsis sp. SD5: A potent feather degrading rare actionobacterium isolated from feather waste in Tamil Nadu, India”. Journal of Basic Microbiology 52 (2012b): 1-10.
  20. Sivakumar T., et al. “Optimization of cultural condition for keratinase production using Bacillus cereus TS1”. Insight Microbiology 3 (2013): 1-8.
  21. Stanbury PF., et al. Principles of fermentation technology, (2nd ed), Aditya Books (P) Ltd. New Delhi, India (1997).
  22. Gupta R., et al. “Bacterial alkaline proteases: molecular approaches and industrial applications”. Applied Microbiology and Biotechnology 59 (2002): 15-32.
  23. Sandhya C., et al. “Comparative evaluation of neutral protease production by Aspergillus oryzae in submerged and solid-state fermentation”. Process Biochemistry 40 (2005): 2689-2694.
  24. Nadeem M., et al. “Effect of medium composition on commercially important alkaline protease production by Bacillus licheniformis N-2”. Food Technology and Biotechnology 46 (2008): 388-394.
  25. Thys R C., et al. “Characterization of a protease of a feather degrading Microbacterium sp”. Letters in Applied Microbiology 39 (2004): 181-186.
  26. Santos R M D B., et al. “Keratinolytic activity of Aspergillus fumigates”. 33 (1996): 364-370.
  27. Yamaamura S., et al. “Characterization of a new keratin degrading bacterium isolates from deer fur”. Journal of Bioscience and Bioengineering 93 (2002): 595-600.
  28. Jeong JH., et al. “Production of keratinolytic enzyme by a newly isolated feather degrading Stenotrophomonas maltophilia that produces plant growth-promoting activity”. Process Biochemistry 45 (2010b): 1738-1745.
  29. Aikat K and Bhattacharyya B C. “Protease extraction in solid-state fermentation of wheat bran by a local strain of Rhizopus oryzae and growth studies by the soft gel technique”. Process Biochemistry 35 (2000): 907-914.
  30. Brandelli A., et al. “Biochemical features of microbial keratinases and their production and applications”. Applied Microbiology and Biotechnology 85 (2010): 1735-1750.
  31. Puri S., et al. “Optimization of alkaline protease production from Bacillus sp. by response surface methodology”. Current Microbiology 44 (2002): 286-290.
×

Citation

Citation: Tooba Momin., et al. “Optimizing the Fermentation Conditions and Enhancing the Keratinase Production from Streptomyces coelicoflavus". Acta Scientific Microbiology 4.3 (2021): 14-24.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days

Indexed In






News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is December 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"

Contact US