Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 4 Issue 1

Genotyping Study for the Promoter of IL-4 Gene in Iraqi Patients with Tuberculosis

Mazin S Salman1* and Awatif H Issa2

1Dip in Psychiatry, Medical Officer, Kandy General Hospital, Sri Lanka
2Senior Registrar in National Hospital of Colombo, Sri Lanka

*Corresponding Author: Mazin S Salman, Ministry of Education, Elite School of Basra, Iraq.

Received: October 03, 2020; Published: December 16, 2020



  Tuberculosis still represents a disaster that puts heavy shadow over all human societies. In spite of the availability of effective chemotherapy and vaccine, tuberculosis is a global health concern for both developing and developed countries. A defect in the genes of the immune response is the most acceptable explanation for susceptibility of some individuals and resistance of others to TB. Cytokines play critical role in interactions and integration between the cells of immune system, which leads to effective defense against TB, among cytokines IL-4, which have regulatory role in immune response in Iraqi patients with TB. Seventy-four blood specimens were collected from 74 patients in the Institute of the Tuberculosis and Chest Disease-Basra city, blood specimens also collected from 74 healthy individuals as control. Extracted DNA was amplified using two sets of specific primers for promoter regions of IL-4. Purified amplicons were sequenced and were analyzed using specific software. The genotyping analysis of promoter of IL-4 gene was indicated two alleles C and T, of them C allele is predominant (73%), while T allele is recessive (24%). Although those heterozygous genotypes (CT) were present in patient and healthy control (HC), but the frequency of CT in patients was significantly higher than those of controls. IL-4 promoters carry more than one type of mutations pushing toward increasing susceptibility of some individuals to TB.

Keywords: Interleukin-4; Tuberculosis; Mycobacterium tuberculosis



  1. Brites D and Gagneux S. “Co-evolution of Mycobacterium tuberculosis and Homo sapiens”. Immunological Reviews 1 (2015): 6-24.
  2. Issa AH., et al. “The Reality of Tuberculosis Disease in Basra Province from 2010 to 2014”. Asian Academic Research Journal of Multidisciplinary 5 (2016): 2319-2801.
  3. AL-Kadhimi HM and Dawood HN. “The Effect of Age on Clinical and Radiological Presentation in Patients with Pulmonary Tuberculosis in Baghdad”. The Iraqi Postgraduate Medical Journal 1 (2011): 125-129.
  4. Rahoud SA., et al. “IL-13 Polymorphism (IL-13 rs1800925 (-1055) (C/T) is Associated with Severe Hepatic Fibrosis in Human chistosomiasis”. Biological and Chemical Research (2015): 11-26.
  5. Casanova JL. “Human genetic basis of interindividual variability in the course of infection”. Proceedings of the National Academy of Sciences of the United States of America 51 (2015): 7118-7127.
  6. Pachecoa AG and Moraes MO. “Genetic polymorphisms of infectious diseases in case-control studies”. Disease Markers 27 (2009): 173-186.
  7. Hanta I., et al. “Association of the Nramp1 gene polymorphisms and clinical forms in patients with tuberculosis”. Bratislava Medical Journal11 (2012): 657-660.
  8. Vidyarani M., et al. “Interferon gamma (IFN-γ) and interleukin-4 (IL-4) gene variants and cytokine levels in pulmonary tuberculosis”. Indian Journal of Medical Research (2006): 403-410.
  9. Butov DO., et al. “Association of interleukins genes polymorphisms with multi-drug resistant tuberculosis in Ukrainian population”. Pneumonologia i Alergologia Polska 84 (2016): 168-173.
  10. Hu Y., et al. “Association between cytokine gene polymorphisms and tuberculosis in a Chinese population in Shanghai: a case-control study”. BMC Immunology 8 (2015): 1-10.
  11. Fol M., et al. “Immune response gene polymorphisms in tuberculosis”. Acta Biochimica Polonica 4 (2015): 633-640.
  12. Jha AN., et al. “IL-4 haplotype -590T, -34T and intron-3 VNTR R2 is associated with reduced malaria risk among ancestral Indian tribal populations”. PLoS One10 (2012): 1-12.
  13. Butov DO. “Influence of IL-2, IL-4 AND IL-10 gene polymorphismon venous blood cytokine synthesis in patients with recurrent pulmonary tuberculosis on standard chemotherapy Ukr”. Pulmonology Journal 1 (2015): 15-17.


Citation: Mazin S Salman and Awatif H Issa. “Genotyping Study for the Promoter of IL-4 Gene in Iraqi Patients with Tuberculosis". Acta Scientific Microbiology 4.1 (2021): 73-76.


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