Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 11

Electron Microscopy Used for the Selection of the Microbial Strains Requested in the Case of Hydrolyzed Wood Wastes Conversion to Fodder Yeasts

Ana- Despina Ionescu*, Angela Căşărică and Nicoleta Ene

National Institute for Chemical- Pharmaceutical Research and Development - Bucharest, Vitan Avenue, Bucharest, Romania

*Corresponding Author: Ana-Despina Ionescu, National Institute for Chemical-Pharmaceutical Research and Development - Bucharest, Vitan Avenue, Bucharest, Romania.

Received: September 17, 2020; Published: October 30, 2020



  According to the literature data, the conversion of the ligno-cellulosic materials towards fodder yeasts involves first the use of some selected microbial strains able to produce the enzymatic factors and then the optimal yeasts. The factors with influence on this bioprocess are obviously the applied parameters but also the morphology of the studied microbial strains. The optimal used parameters can be establish by analytical determinations, but the morphology must be observed by optical microscopy, electron microscopy, X-ray photoelectron spectroscopy, infrared spectroscopy, contact angle, electrophoretic mobility measurements or even by atomic force microscopy. Our studies were realized by electron microscopy on the some previously selected strains of filamentous fungi and yeasts.

Keywords: Electron Microscopy; Wood; Cellulases; Yeasts



  1. Yves F Dufrene. “Atomic Force Microscopy, a Powerful Tool in Microbiology”. Journal of Bacteriology 19 (2002).
  2. Jie Xiaol and Yves F Dufrene. “Optical and force nanoscopy in microbiology”. Nature Microbiology 11 (2016).
  3. Michael Elbaum. “Quantitative Cryo-Scanning Transmission Electron Microscopy of Biological Materials”. Advanced Materials 41 (2018).
  4. Alioscha-Perez M., et al. “A Robust Actin Filaments Image Analysis Framework”. PLOS Computational Biology 8 (2016).
  5. Flavien Pillet., et al. “Uncovering by atomic force microscopy of an original circular structure at the yeast cell surface in response to heat shock”. BMC Biology 6 (2014).
  6. Jean Marie Francois., et al. “Use of atomic force microscopy (AFM) to explore cell wall properties and response to stress in the yeast Saccharomyces cerevisiae”. Current Genetics 4 (2013): 187-196.
  7. Marion Schiavone., et al. “Evidence for a Role for the Plasma Membrane in the Nanomechanical Properties of the Cell Wall as Revealed by an Atomic Force Microscopy Study of the Response of Saccharomyces cerevisiae to Ethanol Stress”. Applied and Environmental Microbiology 15 (2016).
  8. Etienne Dague., et al. “Towards a nanoscale view of fungal surfaces”. Yeast4 (2007): 229-237.
  9. Vincent Dupres., et al. “Microbial nanoscopy: a closer look at microbial cell surfaces”. Trends in Microbiology 9 (2010): 395-405.
  10. Peter Hinterdorfer., et al. “Single-molecule imaging of cell surfaces using near-field nanoscopy”. Accounts of Chemical Research 3 (2012): 327-336.
  11. Daniel J Muller and Yves F Dufrene. “Atomic force microscopy: a nanoscopic window on the cell surface”. Trends in Cell Biology 8 (2011): 461-469.
  12. Yves F Dufrene. “Atomic force microscopy of fungal cell walls: an update”. Yeast8 (2010): 465-471.
  13. Christine G Golding and Lindsey L Lamboo. “The scanning electron microscope in microbiology and diagnosis of infectious disease”. Nature Scientific Reports (2016).
  14. Schiavone M., et al. “AFM dendritips functionalized with molecular probes specific to cell wall polysaccharides as a tool to investigate cell surface structure and organization”. Cell Surfer 5 (2019): 100027.
  15. Cristina Martinez- Torres., et al. “Revealing the assembly of filamentous proteins with scanning transmission electron microscopy”. PLoS One12 (2019).
  16. Goossens KV., et al. “Molecular mechanism of flocculation self-recognition in yeast and its role in mating and survival”. mBio2 (2015).
  17. ATA SCIENTIFIC. “The Applications and Practical Uses of Scanning Electron Microscopes”. ATA SCIENTIFIC Instruments (2019).


Citation: Ana- Despina Ionescu., et al. “Electron Microscopy Used for the Selection of the Microbial Strains Requested in the Case of Hydrolyzed Wood Wastes Conversion to Fodder Yeasts". Acta Scientific Microbiology 3.11 (2020): 95-97.


Acceptance rate30%
Acceptance to publication20-30 days

Indexed In

News and Events

  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is June 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US