Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 11

Production of Bacteriocin Like Compound from Lactic Acid Bacteria and Screening its Therapeutic Potential

Rashmi Gaikwad, Vinal Pardhi* and Valencia D’Souza

National Facility for Biopharmaceuticals, G N Khalsa College, Matunga, Mumbai, India

*Corresponding Author: Vinal Pardhi, National Facility for Biopharmaceuticals, G N Khalsa College, Matunga, Mumbai, India.

Received: September 17, 2020; Published: October 22, 2020

×

Abstract

  Antimicrobial peptides (AMPs) have emerged to be promising alternatives to traditional antibiotics due to their broad spectrum activity. Bacteriocins are AMPs that have shown immense potential for diversified use in the food and pharma industry, agriculture and apiculture. Strains of Lactic Acid Bacteria (LAB) produce a wide variety of AMPs. They are considered safe additives in food which makes them a potential candidate to replace abusive use of therapeutic antibiotics which leads to bacterial antibiotic resistance. In the current work, bacteriocin like compounds (BLC) were isolated from Lactobacillus fermentum and evaluated for their anti-microbial and anti-cancer properties. They were further concentrated by employing acetone precipitation and analysed using Tricine SDS-PAGE technique to determine the peptide nature and molecular weight which was found to be approx. 5 kDa after in-situ antibacterial activity assay. Antimicrobial activity of these peptides was investigated using agar cup diffusion method and showed significant bactericidal activity against B. subtilis, S. aureus, K. pneumonia and S. epidermidis. The anti-cancer studies of the partially purified peptides displayed notable activity against MCF 7 breast cancer cell lines. The bacteriocin like compound isolated from Lactobacillus fermentum exhibits the potential of being a challenging molecule to replace traditional antibiotics if a reproducible, scalable and cost effective process is developed.

>

Keywords: Antimicrobial Peptides; Bacteriocin; Lactobacillus fermentum; Partial Purification; Tricine-PAGE

×

References

  1. E Meade., et al. “Bacteriocins, potent antimicrobial peptides and the fight against multi drug resistant species: Resistance is futile?”. Antibiotics1 (2020).
  2. LM T Dicks., et al. “A review: The fate of bacteriocins in the human gastro-intestinal tract: Do they cross the gut-blood barrier?”. Frontiers in Microbiology 9 (2018): 1-14.
  3. V Juturu and JC Wu. “Microbial production of bacteriocins: Latest research development and applications”. Biotechnology Advances 8 (2018): 2187-2200.
  4. PD Cotter., et al. “Bacteriocins: developing innate immunity for food”. Nature Reviews Microbiology 10 (2005): 777-788.
  5. SC Yang., et al. “Antibacterial activities of bacteriocins: Application in foods and pharmaceuticals”. Frontiers in Microbiology 5 (2014): 1-10.
  6. LD Bemena., et al. “Applications of bacteriocins in food, livestock health and medicine.”. International Journal of Current Microbiology and Applied Sciences 12 (2014): 924-949.
  7. VPASP Rashmi Gaikwad. “Bacteriocins and its applications: an overview”. Asian Journal of Microbiology Biotechnology3 (2018): 96-104.
  8. M Hassan., et al. “Natural antimicrobial peptides from bacteria: Characteristics and potential applications to fight against antibiotic resistance”. Journal of Applied Microbiology4 (2012): 723-736.
  9. HS Abanoz and B Kunduhoglu. “Antimicrobial activity of a bacteriocin produced by enterococcus faecalis kt11 against some pathogens and antibiotic-resistant Bacteria”. Korean Journal for Food Science of Animal Resources 5 (2018): 1064-1079.
  10. MP Zacharof., et al. “Bacteriocins: safe, natural antimicrobials for food preservation”. International Journal of Food Microbiology 1 (2011): 90.
  11. L Silkin., et al. “Spermicidal bacteriocins: Lacticin 3147 and subtilosin A”. Bioorganic and Medicinal Chemistry Letters 10 (2008): 3103-3106.
  12. SS Grosu-Tudor., et al. “Characterization of some bacteriocins produced by lactic acid bacteria isolated from fermented foods”. World Journal of Microbiology and Biotechnology9 (2014): 2459-2469.
  13. S Elayaraja., et al. “Production, purification and characterization of bacteriocin from Lactobacillus murinus AU06 and its broad antibacterial spectrum”. Asian Pacific Journal of Tropical Biomedicine 1 (2014): S305-S311.
  14. S Oh., et al. “Characterization and purification of a bacteriocin produced by a potential probiotic culture, Lactobacillus acidophilus 30SC”. Journal of Dairy Science12 (2000): 2747-2752.
  15. H Schägger. “Tricine-SDS-PAGE”. Nature Protocol1 (2006): 16-22.
  16. T Huang., et al. “Purification and Characterization of a Novel Cold Shock Protein-Like Bacteriocin Synthesized by Bacillus thuringiensis”. Scientific Report 6 (2016): 1-15.
  17. J Zhang., et al. “Purification and partial characterization of bacteriocin Lac-B23, a novel bacteriocin production by lactobacillus plantarumJ23, isolated from Chinese traditional fermented milk”. Frontiers in Microbiology 9 (2018): 1-7.
  18. MP Mokoena. “Lactic acid bacteria and their bacteriocins: Classification, biosynthesis and applications against uropathogens: A mini-review”. Molecules8 (2017).
  19. B Kaur., et al. “Biomedical applications of fermenticin HV6b isolated from lactobacillus fermentum HV6b MTCC10770”. BioMed Research International (2013).
×

Citation

Citation: Vinal Pardhi., et al. “Production of Bacteriocin Like Compound from Lactic Acid Bacteria and Screening its Therapeutic Potential". Acta Scientific Microbiology 3.11 (2020): 42-47.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days

Indexed In






News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is December 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"

Contact US