Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 11

Vancomycin Resistant Enterococci in Makkah Workers

Hussam Aldeen Hassan1*, Abdalla Osman Abdalla Ahmed2 and Bashir Sirag3

1Medical Laboratory Specialist in Zaher Gadeeb Al-Baan Polyclinic, Makkah, Saudi Arabia
2Associate Professor, Department of Medical Microbiology, Faculty of Medicine, Umm Al-Qura University, Saudi Arabia
3Microbiology Department, Faculty of Medicine, Umm Al-Qura University, Saudi Arabia

*Corresponding Author: Hussam Aldeen Hassan, Medical Laboratory Specialist in Zaher Gadeeb Al-Baan Polyclinic, Makkah, Saudi Arabia.

Received: August 17, 2020; Published: October 15, 2020

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Abstract

Aim: The aim of this study is to determine the vancomycin susceptibility in intestinal isolates of Enterococci isolated from healthy workers in Makkah.

Methods: disk diffusion technique was used in this study to determine the antimicrobial susceptibility for Penicillin, Ampicillin, Vancomycin and Linezolid. PCR testing for major resistant genes was also used.

Results: No resistant isolate was detected by disk diffusion technique. However, eight samples were PCR positive for Van B, two isolates were positive for Van C and only one isolate was positive VanC2/C3.

Conclusions: all isolates were sensitive by disc diffusion method, and Van genes were detected by PCR in few numbers of isolates.

Keywords: Enterococci; Streptococci; Ampicillin; Penicillin

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References

  1. Lebreton F., et al. “Enterococcus Diversity, Origins in Nature, and Gut Colonization”. In: GILMORE, M. S., CLEWELL, D. B., IKE, Y. and SHANKAR, N. (eds.) Enterococci: From Commensals to Leading Causes of Drug Resistant Infection. Boston (2014).
  2. Teixeira LM., et al. “Enterococcus. Manual of Clinical Microbiology, 10th Edition”. American Society of Microbiology (2011).
  3. Crouzet L., et al. “Lactobacillus paracasei CNCM I-3689 reduces vancomycin-resistant Enterococcus persistence and promotes Bacteroidetes resilience in the gut following antibiotic challenge”. Scientific Report 8 (2018): 5098.
  4. Cheng M., et al. “The Bacteriophage EF-P29 Efficiently Protects against Lethal Vancomycin-Resistant Enterococcus faecalis and Alleviates Gut Microbiota Imbalance in a Murine Bacteremia Model”. Frontiers in Microbiology 8 (2017): 837.
  5. Summers NA., et al. “Multi-drug-resistant Enterococcus faecium bacteraemia in a liver transplant recipient”. JMM Case Report 6 (2019): e005172.
  6. Al-Ahdal MN., et al. “Characterization of Enterococcus faecium isolates and first report of vanB phenotype-vanA genotype incongruence in the Middle East”. European Journal of Clinical Microbiology and Infectious Diseases 31 (2012): 3223-3229.
  7. Benamu E and Deresinski S. “Vancomycin-resistant enterococcus infection in the hematopoietic stem cell transplant recipient: an overview of epidemiology, management, and prevention”. F1000Research 7 (2018): 3.
  8. Gouliouris T., et al. “Duration of exposure to multiple antibiotics is associated with increased risk of VRE bacteraemia: a nested case-control study”. Journal of Antimicrobial Chemotherapy 73 (2018): 1692-1699.
  9. Abdelkhalek A., et al. “Repurposing ebselen for decolonization of vancomycin-resistant enterococci (VRE)”. PLoS One 13 (2018): e0199710.
  10. Cha JO., et al. “Diversity of Tn1546 in vanA-positive Enterococcus faecium clinical isolates with VanA, VanB, and VanD phenotypes and susceptibility to vancomycin”. Journal of Applied Microbiology 115 (2013): 969-976.
  11. Oravcova V., et al. “Wild corvid birds colonized with vancomycin-resistant Enterococcus faecium of human origin harbor epidemic vanA plasmids”. Environment International 118 (2018): 125-133.
  12. Dutka-Malen S., et al. “Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR”. Journal of Clinical Microbiology 33 (1995): 1434.
  13. Gaca A O and GILMORE MS. “Killing of VRE Enterococcus faecalis by commensal strains: Evidence for evolution and accumulation of mobile elements in the absence of competition”. Gut Microbes 7 (2016): 90-96.
  14. Asadian M., et al. “Antimicrobial resistance pattern and genetic correlation in Enterococcus faecium isolated from healthy volunteers”. Microbial Pathogenesis 92 (2016): 54-59.
  15. Hassan H and SHORMAN M. “Evaluation of the BD GeneOhm MRSA and VanR Assays as a Rapid Screening Tool for Detection of Methicillin-Resistant Staphylococcus aureus and Vancomycin-Resistant Enterococci in a Tertiary Hospital in Saudi Arabia”. International Journal of Microbiology (2011): 861514.
  16. Hegstad K., et al. “Performance of the EUCAST disk diffusion method, the CLSI agar screen method, and the Vitek 2 automated antimicrobial susceptibility testing system for detection of clinical isolates of Enterococci with low- and medium-level VanB-type vancomycin resistance: a multicenter study”. Journal of Clinical Microbiology 52 (2014): 1582-1589.
  17. Hashimoto Y., et al. “Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan”. BMC Microbiology 18 (2018): 213.
  18. Ozbak HA. “A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci”. Annals of Saudi Medicine 38 (2018): 200-207.
  19. Cheesbrough M. “Microbiological tests. District Laboratory Practice in Tropical Countries”. Second Edition ed.: Cambridge University Press (2006).
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Citation

Citation: Hussam Aldeen Hassan., et al. “Vancomycin Resistant Enterococci in Makkah Workers". Acta Scientific Microbiology 3.11 (2020): 24-27.




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