Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Research Article Volume 3 Issue 10

Trends of Entamoeba histolytica Infections in a Tertiary Care Hospital of South India - A Three Year Perspective Study

Gunalan Anitha, Mugunthan M, Nonika Rajkumari* and Gopika Jayan

Department of Microbiology, JIPMER, Puducherry, India

*Corresponding Author: Dr Nonika Rajkumari, Associate Professor, Department of Microbiology, Institute block, JIPMER, Dhanvantri Nagar, Puducherry, India. E-mail:

Received: August 10, 2020; Published: September 16, 2020



Background: Amoebiasis is still a leading cause of intestinal parasitic infections in the world. It can be asymptomatic or manifest a wide variety of presentation and hence its diagnosis of the pathogenic from the non-pathogenic is quite challenging.

Methodology: However, a changing pattern has been observed over the years with this pathogen. This study tried to explore the changing trends in patients infected with this pathogen over a period of 3 years.

Results: E. histolytica microscopy was positive in 55 cases with no gender predisposition. Most of the patients presented with mild abdominal pain associated with diarrhea. In this study we found that there is a decrease in prevalence of infection caused by various Entamoeba species. The overall positivity of infection was 3% during the study period, which could most probably be due to improper sanitation favouring the transmission of the disease.

Conclusion: There has been increasing trend towards the non-pathogenic ones being recovered from the patients. This observation is especially applicable in light of the fact that expanding significance has been given to generally nonpathogenic species such as E. dispar and E. moshkovskii, since invasive amoebiasis has been exhibited to be related with these species. All things considered, the determinants of invasive amoebiasis are complicated and furthermore include different host factors.

Keywords: Amoebiasis; Microscopy; Trends; Entamoeba histolytica



  1. Gunther J., et al. “Amebiasis-related mortality among United States residents, 1990–2007”. American Journal of Tropical Medicine and Hygiene 6 (2011): 1038-1040.
  2. Montague BT., et al. “The immunosuppressed patient”. Infection Disease: A Geo Gui (2011): 374.
  3. Alavi KA. “Amebiasis”. Clinical Colorectal Cancer Surgery 20 (2007): 33-37.
  4. Haque R., et al. “Epidemiologic and clinical characteristics of acute diarrhea with emphasis on Entamoeba histolytica infections in preschool children in an urban slum of Dhaka, Bangladesh”. American Journal of Tropical Medicine and Hygiene 4 (2003): 398-405.
  5. Shirley DA., et al. “A review of the global burden, new diagnostics, and current therapeutics for amebiasis”. Open Forum Infectious Diseases 7 (2018): 161.
  6. Kantor M., et al. “Entamoeba histolytica: Updates in Clinical Manifestation, Pathogenesis, and Vaccine Development”. Canadian Journal of Gastroenterology and Hepatology (2018): 4601420.
  7. Nowak P., et al. “Entamoeba histolytica-pathogenic protozoan of the large intestine in humans”. Journal of Clinical Microbiology and Biochemical Technology 1 (2015): 010-017.
  8. Greene JM., et al. “National Institute of Allergy and Infectious Diseases bioinformatics resource centers: new assets for pathogen informatics”. Infection Immunology7 (2007): 3212-3219.
  9. Khan U., et al. “Detection of Entamoeba histolytica using polymerase chain reaction in pus samples from amebic liver abscess”. Indian Journal of Gastroenterology 2 (2006): 55.
  10. Singh A., et al. “Rapid diagnosis of intestinal parasitic protozoa, with a focus on Entamoeba histolytica”. Interdisciplinary Perspectives on Infectious Diseases (2009).
  11. Fotedar R., et al. “Laboratory diagnostic techniques for Entamoeba species”. Clinical Microbiology Reviews 3 (2007): 511-532.
  12. Blessmann J., et al. “Real-time PCR for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in fecal samples”. Journal of Clinical Microbiology 12 (2002): 4413-4417.
  13. Orosz E., et al. “Real-time PCR assay for rapid qualitative and quantitative detection of Entamoeba histolytica”. Acta Microbiologica Etimmunologica Hungarica 4 (2012): 451-460.
  14. Khairnar K., et al. “Diagnosis of intestinal amoebiasis by using nested polymerase chain reaction-restriction fragment length polymorphism assay”. Journal of Gastroenterology8 (2001): 631-640.
  15. Nath J., et al. “Molecular Epidemiology of Amoebiasis: A Cross-Sectional Study among North East Indian Population”. PLOS Neglected Tropical Diseases 21 (2015): e0004225.
  16. Calegar D., et al. “Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil”. Memórias do Instituto Oswaldo Cruz2 (2016): 114-119.
  17. Shnawa B. “Molecular Diagnosis of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii: An Update Review”. Annual Research and Review in Biology 5 (2017): 1-12.
  18. Sitotaw B., et al. “Prevalence of intestinal parasitic infections and associated risk factors among Jawi primary school children, Jawi town, north-west Ethiopia”. BMC Infection Disease1 (2019): 341.
  19. Sastry A., et al. “Essentials of medical Parasitology”. 2nd Edition (2018).
  20. Mukherjee AK., et al. “Trend of Entamoeba histolytica infestation in Kolkata”. Gut Pathogens1 (2010): 12.


Citation: Gunalan A., et al. “Trends of Entamoeba histolytica Infections in a Tertiary Care Hospital of South India - A Three Year Perspective Study". Acta Scientific Microbiology 3.10 (2020): 38-41.


Acceptance rate30%
Acceptance to publication20-30 days

Indexed In

News and Events

  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is April 30th, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue".
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US