Acta Scientific Microbiology (ASMI) (ISSN: 2581-3226)

Review Article Volume 3 Issue 9

Current and Future Diagnostic Tests for COVID-19 - Challenges and Recommendations

Anju Kaushal*

Assistant Professor (Former), Shiva Group of Institutions Bilaspur, HP, India

*Corresponding Author: Anju Kaushal, Assistant Professor (Former), Shiva Group of Institutions Bilaspur, HP, India.

Received: August 17, 2020; Published: August 26, 2020

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Abstract

The diagnostic tests for COVID-19 are ranged from molecular tests (detect the RNA virus) to serological tests (detect the past infection). Most of the molecular tests are being developed are based on the real- time RT-PCR assay. For conducting PCR assay the number of molecular targets has been identified within the RNA of Corona Virus; such as helicase (Hel), nucleocapsid (N), transmembrane (M), envelop (E) and envelop glycoproteins spike (S). Serological tests are useful in testing past infection in already recovered patients and the convalescent sera of patients with negative PCR findings. These tests are mostly based on the principles of immunochromatography, chemiluminescence or ELISA to detect IgG or IgG and IgM together in serum samples. Serodiagnosis is useful in testing convalescent sera of patients with negative PCR findings. However, the cross reactivity with other antibodies is a major challenge to serological tests. From the second week IgM titre increases and then gradually declines 3rd week onwards, but IgG level remain stable around week 4.

LAMP, CRISPR and multiplex isothermal amplification followed by the microarray detection methods are being developed around the world, to increase the sensitivity and accuracy for virus detection. LAMP Assay could be a potential alternative to RT-PCR in coming future as a point-of -care device assay. DNA amplifies rapidly in isothermal conditions and this tech doesn’t need any specialized equipment. CRISPR-Cas 13 method is also administered, based on specific high-sensitivity enzymatic reporter, unlocks a SHERLOCK protocol for more accuracy and rapidity. NGS is an emerging technology, is used to construct NGS library by amplifying the full length of genes, could be used in epidemiological surveillance and phylogenetic analysis.

These emerging technologies can be implemented to provide better, rapid and accurate diagnosis. These could be used for epidemiological purpose in a wider community, without a need for sophisticated equipment and specific training.

RT- PCR assays help us to tackle this unprecedented outbreak of COVID-19 which has already impacted people’s lives and economy. The rapid tests kits are in high demand for providing services in emergency situations and on the bed side of the patients. These tools are best possible tools providing assistance in saving people’s lives right on time.

Keywords: COVID-19; SARSCoV-2; RT-PCR; IgG; IgM; ELISA; LAMP

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References

  1. Broughton JP., et al. “CRISPR–Cas12-based detection of SARS-CoV-2”. Nature Biotechnology (2020): 1-5.
  2. Bustin SA., et al. “Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction”. Journal of Biomolecular Techniques 15 (2004) 155-166.
  3. Caruana G., et al. “Diagnostic strategies for SARS CoV-2 infection and interpretation of microbiological results”. CMI Clinical Microbiology and Infection (2020).
  4. CDC. US 24/7. “Intrim Guidelines for collecting, handling and testing clinical samples of COVID-19”. (2020).
  5. Centers for Disease Control and Prevention. “CDC real-time RT-PCR diagnostic panel”. CDC Atlanta, GA (2020).
  6. Chan JFW., et al. “Improved molecular diagnosis of COVID-19 by the novel, highly sensitive and specific COVID-19- RdRp/Hel real -time reverse transcription-polymerase chain reaction assay validated in vitro and with clinical specimens”. Journal of Clinical Microbiology 58 (2020) e00310-e00320.
  7. Chávez V., et al. “Sources of pre-analytical, analytical and post-analytical errors in the microbiology laboratory.in: Dasgupta A. Sepulveda J.L. Accurate results in the clinical laboratory”. 2nd ed. A guide to error detection and correction. Elsevier Inc., New York, NY (2019): 377-384
  8. Chiu C. “Cutting-edge infectious disease diagnostics with CRISPR”. Cell Host and Microbe 23 (2018): 702-704.
  9. Corman V.M. “Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR”. Euro Surveillance 25.3 (2020).
  10. Döhla M., et al. “Rapid point-of-care testing for SARS-CoV-2 in a community screening setting shows low sensitivity”. Public Health 182 (2020): 170-172.
  11. Food and Drug Administration Emergency use authorizations (2020).
  12. Fang Y., et al. “Sensitivity of Chest CT for COVID-19: comparison to RT-PCR”. Radiology (2020): 200432.
  13. Guo L., et al. “Profiling early humoral response to diagnose novel coronavirus disease (COVID-19)”. Clinical Infection Disease 71.15 (2020): 778-785.
  14. He JL., et al. “Diagnostic performance between CT and initial real-time RT-PCR for clinically suspected 2019 coronavirus disease (COVID-19) patients outside Wuhan, China”. 168 (2020): 105980.
  15. Huang CL., et al. “Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China”. Lancet 395 (2020): 497-506.
  16. Huffnagle GB., et al “The respiratory tract microbiome and lung inflammation: a two-way street”. Mucosal Immunology 10 (2017): 299-306.
  17. Indian Council of Medical Research, New Delhi. “Performance evaluation of Commercial Kits for Detection of SARSCoV-2 RNA by Real Time PCR.
  18. Jitendra PrasadM., et al. “Laboratory diagnosis of SARS CoV-2- A review on current methods”. Journal of Infection and Public Health 13.7 (2020): 901-905.
  19. Li B., et al. “Discovery of bat coronaviruses through surveillance and probe capture-based next-generation sequencing”. MSphere 5 (2020): e00807-e00819.
  20. Liu Y., et al. “Viral dynamics in mild and severe cases of COVID-19”. Lancet Infection Disease 20.6 (2020): 1-2.
  21. Long QX., et al. “Antibody responses to SARS-CoV-2 in patients with COVID-19”. Nature Medicine 26 (2020): 845-848.
  22. Nalla AK., et al. “Comparative performance of SARS-CoV-2 detection assays using seven different primer/probe sets and one assay kit”. Journal of Clinical Microbiology (2020).
  23. Nguyen T., et al. “A novel coronavirus disease (COVID-19): paving the road for rapid detection and point-of-care diagnosis”. Micromachines 11.3 (2019): 306.
  24. Padoan A., et al. “IgA-Ab response to spike glycoprotein of SARS-CoV-2 in patients with COVID-19: A longitudinal study”. Clinica Chimica Acta 507 (2020): 164-166.
  25. Pan Y., et al. “Serological immunochromatographic approach in diagnosis with SARS-CoV-2 infected COVID-19 patients”. Journal of Infection (2020).
  26. Pang J., et al. “Potential rapid diagnostics, vaccine and therapeutics for 2019 novel coronavirus (2019-nCoV): a systematic review.” Journal of Clinical Medicine 9 (2020): E623.
  27. Park GS., et al. “Development of reverse transcription loop-mediated isothermal amplification assays targeting SARS-CoV-2”. Journal of Molecular Diagnostic (2020).
  28. Peng L., et al. “SARS CoV-2 can be detected in urine, blood, anal swab, and oropharyngeal swabs specimens”. Journal of Medical Virology (2020).
  29. Poljak M., et al. “Clinical evaluation of the cobas SARS-CoV-2 test and a diagnostic platform switch during 48 hours in the midst of the COVID-19 pandemic”. Journal of Clinical Microbiology (2020).
  30. Rahul C Bhoyar., et al. “High throughput detection and genetic epidemiology of SARS CoV-2 using COVIDSeq next generation sequencing”. biRxiv (2020).
  31. Rockett., et al. “Revealing COVID-19 Transmission by SARS-CoV-2 Genome Sequencing and Agent Based Modelling”. bioRxiv (2020).
  32. Sarkale P., et al. “First isolation of SARS CoV-2 from clinical samples in India”. Indian Journal of Medical Research 151.2 (2020): 244-250.
  33. Schrader C., et al. “PCR inhibitors–occurrence, properties and removal”. Journal of Applied Microbiology 113 (2012): 1014-1026.
  34. Sethuraman N., et al. “Interpreting diagnostic tests for SARS CoV-2”. JAMA (2020).
  35. Suhandynata RT., et al. “Longitudinal monitoring of SARS-CoV-2 IgM and IgG seropositivity to detect COVID-19”. Journal of Applied Laboratory Medicine (2020): jfaa079.
  36. Wang W., et al. “Detection of SARS-CoV-2 in different types of clinical specimens”. JAMA (2020). 
  37. Wang Y., et al. “Combination of RT-qPCR testing and clinical features for diagnosis of COVID-19 facilitates management of SARS-CoV-2 outbreak”. Journal of Medical Virology 92 (2020): 538-539.
  38. WHO.int. “Emergencies preparedness, response. WHO guidelines for the global surveillance of severe acute respiratory syndrome (SARS): updated recommendations” (2004).
  39. WHO int. “Laboratory testing for coronavirus disease 2019 (COVID-19) in suspected human cases: interim guidance” (2020).
  40. World Health Organization. “Coronavirus Disease Outbreak”.
  41. Wölfel R., et al. “Virological assessment of hospitalized patients with COVID-2019”. Nature (2020). 
  42. Wu Y., et al. “Multifunctional device for nucleic acid extraction based on magnetic separation and its co-working with liquid handling system for high throughput sample preparation”. Journal of Nanoscience and Nanotechnology 16 (2016): 6919-6924.
  43. Xiao AT., et al. “Profile of specific antibodies to SARS-CoV-2: the first report”. Journal of Infection 81.1 (2020): 147-178.
  44. Yan C., et al. “Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay”. Clinical Microbiology and Infection 26.6 (2020): 773-779.
  45. Zhang F., et al. “A protocol for detection of COVID-19 using CRISPR diagnostics (v.20200321)”.
  46. Zheng S., et al. “Viral load dynamics and disease severity in patients infected with SARS-CoV-2 in Zhejiang province, China, January-March 2020: retrospective cohort study”. BMJ 369 (2020): m1443.
  47. Zhen Z., et al. “A simple magnetic nanoparticles-based viral RNA extraction method for efficient detection of SARS-CoV-2”. BioRxiv (2020).
  48. Zou L., et al. “SARS-CoV-2 viral load in upper respiratory specimens of infected patients”. The New England Journal of Medicine 382 (2020): 1177-1179.
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Citation

Citation: Anju Kaushal. “Current and Future Diagnostic Tests for COVID-19 - Challenges and Recommendations". Acta Scientific Microbiology 3.9 (2020): 103-119.




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